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5-HT1A/5-HT7 receptor interplay: Chronic activation of 5-HT7 receptors decreases the functional activity of 5-HT1A receptor and its сontent in the mouse brain

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Abstract

Serotonin receptors 5-HT1A and 5-HT7 are involved in the development of various psychopathologies. Some data indicate that there is an interplay between 5-HT1A 5-HT7 receptors that could be implicated in the regulation of their function. This work analyzed the effects of chronic 5-HT7 activation on the functional activity of 5-HT7 and 5-HT1A receptors, on the corresponding protein levels, and on the expression of genes encoding 5-HT7 and 5-HT1A receptors in the mouse brain. Chronic administration of the 5-HT7 selective agonist LP44 (20.5 nmol, i.c.v., 14 days) produced considerable desensitization of both 5-HT7 and 5-HT1A receptors. In LP44-treated mice, the hypothermic responses mediated by both 5-HT7 and 5-HT1A receptors were attenuated. Moreover, the levels of 5-HT1A receptor protein in the midbrain and the frontal cortex of LP44-treated mice were significantly decreased. However, the brain levels of 5-HT7 receptor protein did not differ between LP44-treated and control mice. Chronic LP44 treatment did not alter the expression of the 5-HT7 and 5-HT1A receptor genes in all investigated brain structure. These data suggest that 5-HT7 receptors participate in the posttranscriptional regulation of the 5-HT1A receptors functioning.

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Abbreviations

5-HT:

serotonin

TPH2:

tryptophan hydroxylase 2

5-HTT:

serotonin transporter

rPolII:

DNA-dependent RNA polymerase II

RT:

reverse transcription

PCR:

polymerase chain reaction

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 633090, Russia

    E. M. Kondaurova, D. V. Bazovkina & V. S. Naumenko

Authors
  1. E. M. Kondaurova
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  2. D. V. Bazovkina
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  3. V. S. Naumenko
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Correspondence to V. S. Naumenko.

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Original Russian Text © E.M. Kondaurova, D.V. Bazovkina, V.S. Naumenko, 2017, published in Molekulyarnaya Biologiya, 2017, Vol. 51, No. 1, pp. 157–165.

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Kondaurova, E.M., Bazovkina, D.V. & Naumenko, V.S. 5-HT1A/5-HT7 receptor interplay: Chronic activation of 5-HT7 receptors decreases the functional activity of 5-HT1A receptor and its сontent in the mouse brain. Mol Biol 51, 136–142 (2017). https://doi.org/10.1134/S0026893316060108

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  • DOI: https://doi.org/10.1134/S0026893316060108

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