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Functional State of Coelastrella multistriata (Sphaeropleales, Chlorophyta) in an Enrichment Culture

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Abstract—

The functional state of an enrichment culture of a green alga Coelastrella multistriata (Trenkwalder) Kalina et Puncochárová in the course of 60-day cultivation was studied. The concentrations of chlorophylls a, b, and c, total carotenoids, protein, vitamins A and E were determined. The rates of lipid peroxide oxidation in the cells of C. multistriata strain MZ–Ch23 were determined using the concentrations of peroxidation products reacting with 2-thiobarbituric acid. The concentrations of these products were determined in the algal biomass on days 15, 25, and 60 of cultivation in the BBM medium, as well as after initiation of peroxide oxidation with Fe2+. Among the photosynthetic pigments, the highest content (14.82 mg/g dry biomass) was shown for chlorophyll a during the exponential growth phase. The total amount of carotenoids was comparable to the values for other studied Coelastrella strains, 0.74–4.21% of the dry biomass. Strain MZ–Ch23 was shown to accumulate high concentrations of retinol (170.49–586.09 µg/L of the algal culture) and protein (141.81–212.38 mg/L). While during all phases of the cultivation C. multistriata cells exhibited low concentrations of the products of lipid peroxide oxidation (0.005–0.015 nmol/mg protein), initiation of peroxide oxidation increased the concentration of TBA-active products up to 0.187 nmol/mg protein on day 60. Cultivation in BBM medium for 25 days proved to be the optimal strategy for obtaining C. multistriata biomass enriched with secondary metabolites and resistant to peroxide oxidation. This resulted in significant amounts of the biomass with high levels of carotenoids, protein, and vitamin A.

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Funding

The biochemical research was supported by the Russian Science Foundation, project no. 20-74-10076. Light microscopy was obtained within the state assignment of the Ministry of Science and Higher Education of the Russian Federation (theme No. 122042700045-3).

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  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276, Moscow, Russia

    S. Yu. Maltseva, M. S. Kulikovskiy & Y. I. Maltsev

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  1. S. Yu. Maltseva
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  2. M. S. Kulikovskiy
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Correspondence to Y. I. Maltsev.

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Translated by P. Sigalevich

Abbreviation: ROS, reactive oxygen species; Car, carotenoid content; TBAaP, products of peroxide lipid oxidation determined with thiobarbituric acid (TBA-active products); Chl a, chlorophyll a; Chl b, chlorophyll b; Chl c, chlorophyll c.

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Maltseva, S.Y., Kulikovskiy, M.S. & Maltsev, Y.I. Functional State of Coelastrella multistriata (Sphaeropleales, Chlorophyta) in an Enrichment Culture. Microbiology 91, 523–532 (2022). https://doi.org/10.1134/S0026261722601385

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