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Peptidase Activity of Proteinivorax Bacteria and Their Possible Ecological Role in the Microbial Communities of Tanatar Soda Lakes (Altai Krai, Russia)

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Abstract—

Peptidase activity of alkaliphilic anaerobic proteolytic Proteinivorax bacteria, P. tanatarense Z-910T and P. hydrogeniformans Z-710T, isolated from the Tanatar system of hypersaline alkaline lakes (Altai krai, Russia) was studied. Strains Z-910 and Z-710 were shown to hydrolyze para-nitroanilide substrates, exhibiting the highest activity hydrolyzing GlpAALpNA and LpNa. The peptidases were most active under alkaline conditions at pH 8–10 and were stable at temperatures of up to 50°C. The results on inhibitor analysis and substrate specificity of the studied extracellular enzymes suggested their classification as serine peptidases of the subtilisin-like type (strain Z-910) and metallopeptidases of the aminopeptidase type (strain Z-710).

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Funding

The work was supported by the Russian Foundation for Basic Research, project no. 18-04-00236, partially within the framework of the State Assignment to Research Center of Biotechnology, Russian Academy of Sciences under funding by the Russian Federation Ministry of Science and Higher Education, and partially within the framework of the State Assignment AAAA-A17-117011810034-9 for Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences.

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Authors and Affiliations

  1. Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences, 670047, Ulan-Ude, Russia

    E. V. Lavrentyeva & E. B. Erdyneeva

  2. Banzarov Buryat State University, 670000, Ulan Ude, Russia

    E. V. Lavrentyeva

  3. Moscow State University, 119991, Moscow, Russia

    Ya. E. Dunaevskii

  4. Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    Yu. V. Boltyanskaya & V. V. Kevbrin

Authors
  1. E. V. Lavrentyeva
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  2. E. B. Erdyneeva
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  3. Ya. E. Dunaevskii
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  4. Yu. V. Boltyanskaya
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  5. V. V. Kevbrin
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Correspondence to E. V. Lavrentyeva.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Lavrentyeva, E.V., Erdyneeva, E.B., Dunaevskii, Y.E. et al. Peptidase Activity of Proteinivorax Bacteria and Their Possible Ecological Role in the Microbial Communities of Tanatar Soda Lakes (Altai Krai, Russia). Microbiology 88, 773–776 (2019). https://doi.org/10.1134/S0026261719060079

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  • DOI: https://doi.org/10.1134/S0026261719060079

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