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Regulation of Formation of Monospecies and Binary Biofilms by Human Skin Microbiota Components, Staphylococcus epidermidis and Staphylococcus aureus, by Human Natriuretic Peptides

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Abstract—

Human natriuretic peptides (NUPs) types A and C were shown have different effects on the growth of monospecies and binary biofilms of human skin commensal microorganisms Staphylococcus epidermidis and Staphylococcus aureus. The effect of natriuretic peptides depended on cultivation conditions: at 37°С growth of monospecies biofilms formed by S. epidermidis and S. aureus was stimulated and inhibited, respectively, resulting in changed values of the biofilm average thickness and biomass in presence of NUPs. At 33°С effects of NUPs reversed: growth of S. epidermidis monospecies biofilms was inhibited. In binary biofilms at 37°С, NUPs were able to increase the competitiveness of S. epidermidis against S. aureus. NUPs affected predominantly biofilms and, to a lesser degree, planktonic cultures. This phenomenon makes it possible to consider NUPs as the molecules able to regulate the interactions between the human organism and skin microbiota.

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ACKNOWLEDGMENTS

The research on cell viability within biofilms was supported by a grant of the Russian Fundation for Basic Research (Project No. 14-50-00029). The studies on the composition of binary biofilms, the dynamics of culture growth, and the microscopic structure of biofilms were supported by FEDER(UE), Region Normandy, and Evreux Porte de Normandie.

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Authors and Affiliations

  1. Lomonosov Moscow State University, Moscow, Russia

    A. V. Gannesen & A. I. Netrusov

  2. Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia

    A. V. Gannesen & V. K. Plakunov

  3. Laboratory of Microbiology Signals and Microenvironment LMSM EA4312 (Laboratoire de microbiologie signaux et microenvironnement LMSM EA4312), Rouen University, Evreux, France

    A. V. Gannesen, O. Lesouhaitier & M. G. J. Feuilloley

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  1. A. V. Gannesen
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  2. O. Lesouhaitier
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  3. A. I. Netrusov
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  4. V. K. Plakunov
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  5. M. G. J. Feuilloley
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Correspondence to A. V. Gannesen.

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Translated by A. Oleskin

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Gannesen, A.V., Lesouhaitier, O., Netrusov, A.I. et al. Regulation of Formation of Monospecies and Binary Biofilms by Human Skin Microbiota Components, Staphylococcus epidermidis and Staphylococcus aureus, by Human Natriuretic Peptides. Microbiology 87, 597–609 (2018). https://doi.org/10.1134/S0026261718050090

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