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Isolation of primary osteoblast cell lines from adult rat and rat embryos and their use as models for in vitro biocompatibility tests of nanostructured titanium-based implants

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Abstract

Methods for obtaining osteoblast cultures from the calvaria of adult Wistar rats and 12-day-old embryos of these rats have been adapted for studying the biocompatibility and ossointegration of titanium-based implants. The osteoblast morphology and their differentiation into osteocytes on a titanium matrix with specially treated surface have been studied. It has been confirmed that two cultures of diploid rat cells obtained in the study can serve as efficient models for preclinical in vitro testing of nanostructured titanium implants for biocompatibility and osseointegration.

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Authors and Affiliations

  1. Ural Federal University, Yekaterinburg, 620002, Russia

    Y. Y. Khrunyk, A. V. Korelin, S. V. Belikov, M. S. Karabanalov, R. V. Kamalov & A. A. Popov

  2. Ural Scientific Research Veterinary Institute, Yekaterinburg, 620000, Russia

    I. V. Vyalykh

  3. Institute of Plant and Animal Ecology, Ural Branch, Russian Academy of Sciences, Yekaterinburg, 620144, Russia

    Y. Y. Khrunyk & S. B. Rakitin

Authors
  1. Y. Y. Khrunyk
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  2. I. V. Vyalykh
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  3. A. V. Korelin
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  4. S. V. Belikov
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  5. M. S. Karabanalov
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  6. S. B. Rakitin
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  7. R. V. Kamalov
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  8. A. A. Popov
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Corresponding author

Correspondence to Y. Y. Khrunyk.

Additional information

The article was translated by the authors.

Original Russian Text © Y.Y. Khrunyk, I.V. Vyalykh, A.V. Korelin, S.V. Belikov, M.S. Karabanalov, S.B. Rakitin, R.V. Kamalov, A.A. Popov, 2017, published in Doklady Akademii Nauk, 2017, Vol. 475, No. 2, pp. 226–230.

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Khrunyk, Y.Y., Vyalykh, I.V., Korelin, A.V. et al. Isolation of primary osteoblast cell lines from adult rat and rat embryos and their use as models for in vitro biocompatibility tests of nanostructured titanium-based implants. Dokl Biol Sci 475, 175–179 (2017). https://doi.org/10.1134/S0012496617040032

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  • DOI: https://doi.org/10.1134/S0012496617040032

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