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Isolation, cultivation and characterisation of pigeon osteoblasts seeded on xenogeneic demineralised cancellous bone scaffold for bone grafting

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Abstract

Avian osteoblasts have been isolated particularly from chicken embryo, but data about other functional tissue sources of adult avian osteoblast precursors are missing. The method of preparation of pigeon osteoblasts is described in this study. We demonstrate that pigeon cancellous bone derived osteoblasts have particular proliferative capacity in vitro in comparison to mammalian species and developed endogenous ALP. Calcium deposits formation in vitro was confirmed by alizarin red staining. Only a few studies have attempted to investigate bone grafting and treatment of bone loss in birds. Lack of autologous bone grafts in birds has prompted investigation into the use of avian xenografts for bone augmentation. Here we present a method of xenografting of ostrich demineralised cancellous bone scaffold seeded with allogeneic adult pigeon osteoblasts. Ostrich demineralised cancellous bone scaffold supported proliferation of pigeon osteoblasts during two weeks of co - cultivation in vitro. Scanning electron microscopy demonstrated homogeneous adult pigeon osteoblasts attachment and distribution on the surface of xenogeneic ostrich demineralised cancellous bone. Our preliminary in vitro results indicate that demineralised cancellous bone from ostrich tibia could provide an effective biological support for growth and proliferation of allogeneic osteoblasts derived from cancellous bone of pigeons.

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Acknowledgments

This study has been supported by VEGA grant No. 1/0631/09, VEGA grant No.1/0772/13 and Centre of Excellence for Neuroregenerative Research (project ITMS No. 26220120063).

Ethical standards

The experiments on animals were performed at the University of Veterinary Medicine and Pharmacy in Kosice, Slovakia, respecting the guidelines for animal experiments with the approval of the University’s ethical committee.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Associated Tissue Bank of Faculty of Medicine of P. J. Safárik University and L. Pasteur University Hospital, Trieda SNP 1, SK-041 66, Kosice, Slovakia

    Denisa Harvanová, Judita Amrichová, Tímea Spaková, Jana Plsíková & Ján Rosocha

  2. University of Veterinary Medicine and Pharmacy in Kosice, Komenského 73, SK-041 81, Kosice, Slovakia

    Slavomír Hornák & Valent Ledecký

  3. Institute of Biology and Ecology, Faculty of Sciences, P.J. Safárik University in Kosice, Moyzesova 11, 040 01, Kosice, Slovakia

    Jaromír Mikes

Authors
  1. Denisa Harvanová
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  2. Slavomír Hornák
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  3. Judita Amrichová
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  4. Tímea Spaková
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  6. Jana Plsíková
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  7. Valent Ledecký
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  8. Ján Rosocha
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Correspondence to Denisa Harvanová.

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Harvanová, D., Hornák, S., Amrichová, J. et al. Isolation, cultivation and characterisation of pigeon osteoblasts seeded on xenogeneic demineralised cancellous bone scaffold for bone grafting. Vet Res Commun 38, 221–228 (2014). https://doi.org/10.1007/s11259-014-9607-0

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  • DOI: https://doi.org/10.1007/s11259-014-9607-0

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