Abstract
A change in the content of energy substrates in myocardial mitochondria depending upon the vibration frequency and the duration of the total vertical vibration exposure was studied polarographically using a closed galvanic oxygen sensor (a Clark electrode) by comparing the endogenous respiration rates over a period when the homogenate was stored under aerobic conditions. After exposure to the longest vibration (56 sessions) with amplitude of 0.5 mm and frequency of 44 Hz, the rate of endogenous respiration after a 40-min incubation of myocardial tissue homogenate was 1.4 times slower (p < 0.05) than the control. This indicated the development of bottlenecking in the oxidation and utilization pathways of succinic acid, a decrease in the availability of endogenous substrates to the respiratory chain of cardiomyocytes mitochondria and, indirectly, mitochondrial-reticular network disruption and dysfunction.
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Funding
This work was financially supported by the Russian Foundation for Basic Research (no. 10-04-00473, no. 13-04-00186).
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The authors declare that they have no conflict of interest.
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All applicable international, national and institutional guidelines for the care and use of animals have been complied with in the performance of the work. The studies were carried out in accordance with the methodological guidelines and regulatory documents, the rules of laboratory practice for conducting preclinical studies in the Russian Federation (GOST R 53434-2009) and Directive 2010/63/EU of the European Parliament and the Council of the European Union on the protection of animals used for scientific purposes dated September 22, 2010.
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Vorobieva, V.V., Shabanov, P.D. A Change in the Content of Endogenous Energy Substrates in Rabbit Myocardium Mitochondria Depending upon Frequency and Duration of Vibration. BIOPHYSICS 66, 610–613 (2021). https://doi.org/10.1134/S0006350921040229
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DOI: https://doi.org/10.1134/S0006350921040229