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Cell Volume Regulation Monitored with Combined Epifluorescence and Digital Holographic Microscopy

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Neuronal Cell Death

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1254))

Abstract

Quantitative phase imaging emerged recently as a valuable tool for cell observation, by enabling label-free imaging through the intrinsic phase-contrast provided by transparent living cells , thus greatly simplifying observation protocols. The quantitative phase signal , unlike the one provided by the widely used phase-contrast microscope , can be related to relevant biological indicators including dry mass , cell volume regulation or transmembrane water movements . Here, we present quantitative phase imaging coupled with live fluorescence , making it possible to follow the phase signal in time to monitor the cell volume regulation , an early indicator of cell viability , along with specific information such as intracellular Ca2+ imaging with Fura-2 ratiometric fluorescence .

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Acknowledgements

This research was supported by the Swiss National Science Foundation grant #CR32I3-132993 and by the Commission for Technology and Innovation (CTI/KTI) project #9389.1.

Author information

Authors and Affiliations

  1. Biophotonics Laboratory, Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, 565-0871, Japan

    Nicolas Pavillon

  2. Microvision and Microdiagnostics Group (MVD), STI, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland

    Nicolas Pavillon

  3. Département de Psychiatrie, Centre de Neurosciences Psychiatriques, Centre Hospitalier Universitaire Vaudois (CHUV), Site de Cery, CH-1008, Prilly/Lausanne, Switzerland

    Pierre Marquet

  4. Brain Mind Institute (BMI), Ecole Polytechnique Fédérale Lausanne (EPFL), Lausanne, Switzerland

    Pierre Marquet

Authors
  1. Nicolas Pavillon
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  2. Pierre Marquet
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Corresponding author

Correspondence to Nicolas Pavillon .

Editor information

Editors and Affiliations

  1. Dept. of Veterinary Sciences, University of Torino, Grugliasco, Torino, Italy

    Laura Lossi

  2. Dept. of Veterinary Sciences, University of Torino, Grugliasco, Torino, Italy

    Adalberto Merighi

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Pavillon, N., Marquet, P. (2015). Cell Volume Regulation Monitored with Combined Epifluorescence and Digital Holographic Microscopy. In: Lossi, L., Merighi, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 1254. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2152-2_2

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  • DOI: https://doi.org/10.1007/978-1-4939-2152-2_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2151-5

  • Online ISBN: 978-1-4939-2152-2

  • eBook Packages: Springer Protocols

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