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New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids

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Abstract

It was demonstrated for the first time that immobilized lysozyme can efficiently remove Escherichia coli and Pseudomonas aeruginosa lipopolysaccharides (endotoxins) from solutions. Experimentally confirmed sorption capacity for the developed sorbent was at least 400 ng of endotoxin per ml sorbent. The new sorbent is compatible with the whole human blood and can be potentially used in extracorporeal therapy in the treatment of sepsis.

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Authors and Affiliations

  1. Lomonosov Moscow State University, Faculty of Chemistry, 119234, Moscow, Russia

    P. A. Levashov, D. A. Matolygina & N. L. Eremeev

  2. Bauman Moscow State Technical University, Interindustry Engineering Center for Composite Materials, 105005, Moscow, Russia

    P. A. Levashov, D. A. Matolygina, A. V. Nuzhdina & N. S. Pokrovsky

  3. National Medical Research Center of Cardiology, Institute of Experimental Cardiology, Ministry of Healthcare of the Russian Federation, 121359, Moscow, Russia

    E. D. Ovchinnikova & O. A. Dmitrieva

  4. POKARD Ltd, 121359, Moscow, Russia

    I. Yu. Adamova

  5. Lomonosov Moscow State University, Faculty of Fundamental Medicine, 119991, Moscow, Russia

    N. S. Pokrovsky

Authors
  1. P. A. Levashov
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  2. D. A. Matolygina
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  3. E. D. Ovchinnikova
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  4. I. Yu. Adamova
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  5. O. A. Dmitrieva
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  6. A. V. Nuzhdina
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  7. N. S. Pokrovsky
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  8. N. L. Eremeev
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Corresponding author

Correspondence to P. A. Levashov.

Additional information

Published in Russian in Biokhimiya, 2019, Vol. 84, No. 1, pp. 100–108.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM18-208, October 22, 2018.

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Levashov, P.A., Matolygina, D.A., Ovchinnikova, E.D. et al. New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids. Biochemistry Moscow 84, 33–39 (2019). https://doi.org/10.1134/S0006297919010048

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  • DOI: https://doi.org/10.1134/S0006297919010048

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