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Minicircle kinetoplast genome of insect trypanosomatid Leptomonas pyrrhocoris

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Abstract

We present here the structure of a minicircle population based on transcriptome sequencing of Leptomonas pyrrhocoris. We show that minicircle DNA molecules are dimeric. As in dixenous species, the entire molecule of minicircle DNA is transcribed. This is the first minicircle transcriptome of monoxenous trypanosomatid species determined using NGS technology.

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Abbreviations

CR:

conservative region

CSB:

conserved sequence block

gRNA:

guide RNA

kp:

kinetoplast

kpDNA:

kinetoplast DNA

kpRNA:

kinetoplast RNA

mt:

mitochondrial

NGS:

next generation sequencing

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Authors and Affiliations

  1. Faculty of Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    E. S. Gerasimov, A. A. Gasparyan, I. A. Litus & A. A. Kolesnikov

  2. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    M. D. Logacheva

Authors
  1. E. S. Gerasimov
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  2. A. A. Gasparyan
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  3. I. A. Litus
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  4. M. D. Logacheva
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  5. A. A. Kolesnikov
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Correspondence to E. S. Gerasimov or A. A. Kolesnikov.

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Original Russian Text © E. S. Gerasimov, A. A. Gasparyan, I. A. Litus, M. D. Logacheva, A. A. Kolesnikov, 2017, published in Biokhimiya, 2017, Vol. 82, No. 5, pp. 768-775.

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Gerasimov, E.S., Gasparyan, A.A., Litus, I.A. et al. Minicircle kinetoplast genome of insect trypanosomatid Leptomonas pyrrhocoris . Biochemistry Moscow 82, 572–578 (2017). https://doi.org/10.1134/S0006297917050054

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  • DOI: https://doi.org/10.1134/S0006297917050054

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