Abstract
Medical bioremediation is a unique strategy of targeting pathogenic compounds with an exogenous enzyme of microbial origin. The objective of this study was to isolate and screen the microorganisms from diverse environmental samples for their ability to catabolize 7-ketocholesterol. Isolation of bacterial strains was performed and molecular identification was carried out by amplification and sequencing of 16S rDNA for 4 the best degrader isolates. Degradation was confirmed on the basis of UV spectrophotometric and HPLC analysis. Four bacterial isolates, showing high catabolic activity towards 7-ketocholesterol were isolated: Alcanivorax jadensis IP4 (accession number KP309836; sea water sediment), Streptomyces auratus IP2 (accession number KP309837; soil), Serratia marcescens IP3 (accession number KP309838; soil) and Thermobifida fusca IP1 (accession number KM677184; manure piles). All the isolates were capable of utilizing 7-ketocholesterol as the sole organic substrate, resulting in its mineralisation. The most rapid degradation was observed with A. jadensis IP4 followed by T. fusca IP1. The degradation was followed and analyzed by HPLC. A. jadensis IP4 removed 7-ketocholesterol below detection levels within 8 days.
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Krause, M.R. and Regen, S.L., Accounts Chem. Res., 2014, vol. 47, no. 12, pp. 3512–3521.
Brown, A.J., Ikonen, E., and Olkkonen, V.M. Curr. Opin. Lipidol., 2014, vol. 25, pp. 133–139.
Cholesterol and Phytosterol Oxidation Products: Analysis, Occurrence, and Biological Effects, Grandgirard, A., Guardiola, F., Dutta, P., Codony, R., and Savage, G., Eds., Champaign, IL: AOCS Press, 2002.
Colles, S.M., Maxson, J.M., Carlson, S.G., and Chisolm, G.M., Trends Cardiovasc. Med., 2001, vol. 11, pp. 131–138.
Björkhem, I., Heverin, M., Leoni, V., Meaney, S., and Diczfalusy, U., Acta Neurol. Scand., 2006, vol. 114, pp. 43–49.
Vaya, J. and Schipper, H.M., J. Neurochem., 2007, vol. 102, pp. 1727–1737.
Rantham P.J.P., Feist, G., Thomasson, S., Thompson, A., Schommer, E., and Ghribi, O., J. Neurochem., 2008, vol. 107, pp. 1722–1729.
Rodríguez, I.R. and Larrayoz, I.M., J. Lipid Res., 2010, vol. 51, pp. 2847–2862.
Girao, H., Mota, M. C., Ramalho, J., and Pereira, P., Exp. Eye Res., 1998, vol. 66, pp. 645–652.
Lemaire–Ewing, S., Prunet, C., Montange, T., Vejux, A., Berthier, A., Bessède, et al., Cell Biol.Toxicol., 2005, vol. 21 no. 2, pp. 97–114.
Huang, J.D., Amaral, J., Lee, J.W., and Rodriguez, I.R., PLoS One, 2014, vol. 9, no. 7. pp. 1–26.
Dreizen, S., Stern, M.H., and Levy, B.M., J. Dental Res., 1978, vol. 57, pp. 412–417.
Garcia-Cruset, S., Carpenter, K.L., Guardiola, F., Stein, B.K., and Mitchinson M.J., Free Rad. Res., 2001, vol. 35, pp. 31–41.
Moreira, E.F., Larrayoz, I.M., Lee, J.W., and Rodriguez, I.R., Invest. Ophthalmol. Visual Sci., 2009, vol. 50, pp. 523–532.
Leonarduzzi, G., Sottero, B., and Poli G.T., J. Nut. Biochem., 2002, vol. 13, no. 13 pp. 700–710.
Indaram, M., Ma, W., Zhao, L., Fariss, R.N., Rodriguez, I.R., and Wong, W.T., Sci. Reports, 2015, vol. 5, p. 9144.
Vejux, A., Kahn, E., Dumas, D., Bessède, G., Ménétrier, F., Athias, A., et al., Cytometry A, 2005, vol. 64, pp. 87–100.
Zhang, H., Wang, Y., Lin, N., Yang, R., Qiu, W., Han, L., et al., Orphanet. J. Rare Dis., 2014, vol. 9, pp. 82–92.
Perveen, I., Sehar, S., Naz, I., Raza, M.A., Khan, A.J., and Ahmed, S., Int. J. Biosci., 2016, vol. 8, no. 4, pp. 83–93.
Schloendorn, J., Webb, T., Kemmish, K., Hamalainen, M., Jackemeyer, D., Jiang, L., et al., Rejuvenation Res., 2009, vol. 12, no. 6, pp, 411–419.
de Grey, A.D., Alvarez, P.J., Brady, R.O., Cuervo, A.M., Jerome, W.G., McCarty, P.L., et al., Ageing Res. Rev., 2005, vol. 4, no. 3, pp. 315–338.
Tian, J., Gu, X., Sun, Y., Ban, X., Xiao, Y., Hu, S. and Yu, B., BMC Cardiovasc. Disorders, 2012, vol. 12. pp.70–81.
Kostka, J.E., Prakash, O., Overholt, W.A., Green, S.J., Freyer, G., Canion, A., Delgardio, J., et al., Appl. Environ. Microbiol., 2011, vol. 77, no. 22, pp. 7962–7974.
Cappello, S., Denaro, R., Genovese, M., Giuliano, L., and Yakimov, M.M., Microbiol. Res., 2006, vol. 162, no. 2, pp. 185–190.
Kasai, Y., Kishira, H., Sasaki, T., Syutsubo, K., Watanabe, K., and Harayama S., Environ. Microbiol., 2002, vol. 4, no. 3, pp. 141–147.
Liu, C. and Shao, Z., Int. J. Syst.Evol. Microbiol., 2005, vol. 55, pp. 1181–1186.
Golyshin, P.N., Martins Dos Santos, V.A., Kaiser, O., Ferrer, M., Sabirova, Y.S., Lunsdorf, H., et al., J. Biotechnol., 2003, vol. 106, pp. 215–220.
Dutta, T. K. and Harayama, S., Appl. Environ. Microbiol., 2001, vol. 67, pp. 1970–1974.
Qiao, N. and Shao, Z., Appl. Environ. Microbiol., 2010, vol. 108, pp. 1207–1216.
Yakimov, M.M., Golyshin, P.N., Lang, S., Moore, E.R., Abraham, W.R., Lunsdorf, H., and Timmis, K.N., Int. J. Syst. Bacteriol., 1998, vol. 48, pp. 339–348.
Mathieu, J.M., Mohn, W.W., Eltis, L.D., LeBlanc, J.C., Stewart, G.R., Dresen, C., et al., Appl. Environ. Microbiol., 2010, vol. 76, no. 1, pp. 352–355.
Mathieu, J., Schloendorn, J., Rittmann, B.E., and Alvarez, P.J., Biodegradation. 2008, vol. 19, no. 6. pp. 807–813.
Ghosh S. and Khare. S.K., Chem. Phys. Lipids, 2017, vol. 207, Part B, pp. 253–259.
Ghosh, S. and Khare, S.K., Biores. Technol., 2016, vol. 213, pp. 44–49.
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Perveen, I., Raza, M.A., Sehar, S. et al. Studies on Degradation of 7-ketocholesterol by Environmental Bacterial Isolates. Appl Biochem Microbiol 54, 262–268 (2018). https://doi.org/10.1134/S0003683818030110
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DOI: https://doi.org/10.1134/S0003683818030110