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PEGylated recombinant L-asparaginase from Erwinia carotovora: Production, properties, and potential applications

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Abstract

N-hydroxysuccinimide ester of monomethoxy polyethylene glycol hemisuccinate was synthesized. It acylated amino groups in a molecule of recombinant L-asparaginase from Erwinia carotovora. A method of L-asparaginase modification by the obtained activated polyethylene glycol derivative was developed. The best results were produced by modification of the enzyme with a 25-fold excess of reagent relative to the enzyme tetramer. The modified L-asparaginase was isolated from the reaction mixture by gel filtration on Sepharose CL-6B. The purified bioconjugate did not contain PEG unbound to the protein, demonstrated high catalytic activity, and exhibited antiproliferative action on cell cultures.

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Authors and Affiliations

  1. Department of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russia

    N. S. Melik-Nubarov, I. D. Grozdova & G. Yu. Lomakina

  2. Orekhovich Scientific Research Institute of Biomedical Chemistry, Moscow, 119121, Russia

    M. V. Pokrovskaya, V. S. Pokrovski, S. S. Aleksandrova, O. Yu. Abakumova, O. V. Podobed, D. V. Grishin & N. N. Sokolov

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  1. N. S. Melik-Nubarov
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  2. I. D. Grozdova
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  3. G. Yu. Lomakina
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  4. M. V. Pokrovskaya
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  5. V. S. Pokrovski
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  6. S. S. Aleksandrova
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  7. O. Yu. Abakumova
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  8. O. V. Podobed
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  9. D. V. Grishin
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  10. N. N. Sokolov
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Correspondence to N. S. Melik-Nubarov.

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Original Russian Text © N.S. Melik-Nubarov, I.D. Grozdova, G.Yu. Lomakina, M.V. Pokrovskaya, V.S. Pokrovski, S.S. Aleksandrova, O.Yu. Abakumova, O.V. Podobed, D.V. Grishin, N.N. Sokolov, 2017, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2017, Vol. 53, No. 2, pp. 164–172.

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Melik-Nubarov, N.S., Grozdova, I.D., Lomakina, G.Y. et al. PEGylated recombinant L-asparaginase from Erwinia carotovora: Production, properties, and potential applications. Appl Biochem Microbiol 53, 165–172 (2017). https://doi.org/10.1134/S0003683817020119

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  • DOI: https://doi.org/10.1134/S0003683817020119

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