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Suppression of T24 human bladder cancer cells by ROS from locally delivered hematoporphyrin-containing polyurethane films

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Abstract

Systemic injection of a photosensitizer is a general method in photodynamic therapy, but it has complications due to the unintended systemic distribution and remnants of photosensitizers. This study focused on the possibility of suppressing luminal proliferative cells by excessive reactive oxygen species from locally delivered photosensitizer with biocompatible polyurethane, instead of the systemic injection method. We used human bladder cancer cells, hematoporphyrin as the photosensitizer, and polyurethane film as the photosensitizer-delivering container. The light source was a self-made LED (510 nm, 5 mW cm−2) system. The cancer cells were cultured on different doses of hematoporphyrin-containing polyurethane film and irradiated with LED for 15 minutes and 30 minutes each. After irradiating with LED and incubating for 24 hours, cell viability analysis, cell cycle analysis, apoptosis assay, intracellular and extracellular ROS generation study and western blot were performed. The cancer cell suppression effects of different concentrations of the locally delivered hematoporphyrin with PDT were compared. Apoptosis dominant cancer cell suppressions were shown to be hematoporphyrin dose-dependent. However, after irradiation, intracellular ROS amounts were similar in all the groups having different doses of hematoporphyrin, but these values were definitely higher than those in the control group. Excessive extracellular ROS from the intended, locally delivered photosensitizer for photodynamic treatment application had an inhibitory effect on luminal proliferative cancer cells. This method can be another possibility for PDT application on contactable or attachable lesions.

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Acknowledgements

This research was supported by the Bio & Medical Technology Development Program of the National Research Foundatio (NRF) funded by the Ministry of Science & ICT of the Korean government (No. 2015M3A9E2028643).

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Authors and Affiliations

  1. Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Korea

    Dohyun Kim, Mi Hee Lee, Min-Ah Koo, Byeong-Ju Kwon, Min Sung Kim, Gyeung Mi Seon, Seung Hee Hong & Jong-Chul Park

  2. Department of Family Medicine, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Korea

    Dohyun Kim

  3. Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Korea

    Min-Ah Koo, Byeong-Ju Kwon, Min Sung Kim, Gyeung Mi Seon, Seung Hee Hong & Jong-Chul Park

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  1. Dohyun Kim
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Correspondence to Jong-Chul Park.

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Kim, D., Lee, M.H., Koo, MA. et al. Suppression of T24 human bladder cancer cells by ROS from locally delivered hematoporphyrin-containing polyurethane films. Photochem Photobiol Sci 17, 763–772 (2018). https://doi.org/10.1039/c7pp00424a

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