Abstract
Heterogeneous reaction of gas phase NO2 with atmospheric humic-like substances (HULIS) is potentially an important source of volatile organic compounds (VOCs) including nitrogen (N)-containing compounds, a class of brown carbon of emerging importance. However, the role of ubiquitous water-soluble aerosol components in this multiphase chemistry, namely nitrate and iron ions, remains largely unexplored. Here, we used secondary electrospray ionization ultrahigh-resolution mass spectrometry for real-time measurements of VOCs formed during the heterogeneous reaction of gas phase NO2 with a solution containing gallic acid (GA) as a proxy of HULIS at pH 5 relevant for moderately acidic aerosol particles. Results showed that the number of detected N-containing organic compounds largely increased from 4 during the NO2 reaction with GA in the absence of nitrate and iron ions to 55 in the presence of nitrate and iron ions. The N-containing compounds have reduced nitrogen functional groups, namely amines, imines and imides. These results suggest that the number of N-containing compounds is significantly higher in deliquescent aerosol particles due to the influence of relatively higher ionic strength from nitrate ions and complexation/redox reactivity of iron cations compared to that in the dilute aqueous phase representative of cloud, fog, and rain water.
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Find the latest articles, discoveries, and news in related topics.Introduction
Atmospheric humic-like substances (HULIS) originate from biomass burning and secondary chemistry1,2 and comprise 48–57% of water-soluble organic compounds in aerosol particles3 with concentrations varying from 0.08 μg m−3 in a marine environment to 28 μg m−3 in a rural area affected by forest fires4,5. HULIS is an important class of brown carbon (BrC), defined as sunlight absorbing organics capable of influencing the radiative forcing on regional and global scales6. Primary sources of BrC are mainly biomass burning7. Secondary sources of BrC from multiphase chemistry in the atmosphere2 are an active area of ongoing research. For example, the oxidation of volatile organic compounds (VOCs) by atmospheric oxidants, especially in the presence of NO2, would lead to the formation of BrC1. Given the importance of secondary organic aerosols (SOA) in affecting air quality, aerosol-cloud, and aerosol-radiation interactions in atmosphere8, studies to date showed that the chemical diversity and physical properties of atmospheric aerosol particles influence the pathways that lead to BrC in SOA9.
The heterogeneous reaction of HULIS with hydroxyl and nitrate radicals can promote the formation of SOA yielding the nitrated compounds10. Nitro-aromatic compounds are typically detected in aerosol particles11. Oxidation of HULIS by NO3 leads to the formation of nitro-containing products, including nitrophenol, nitrated diphenyl, and nitro-products from non-aromatic/ring-opening12. Enhanced photosensitized uptake of NO2 on wet humic acid thin films was studied and found to form HONO13,14. A recent study by Gen et al.15 also reported the photogeneration of nitrite/HONO from the photolysis of iron–organic complexes in the presence of nitrate. However, other gas and condensed phase BrC products from the heterogeneous reaction of HULIS with NO2 have yet to be identified, particularly under experimental conditions that simulates the complex physicochemical properties of atmospheric aerosol particles of liquid water content, pH, ionic strength, and presence of reactive transition metals such as iron, Fe.
Mineral dust is a dominant source of Fe in atmospheric aerosols in addition to combustion sources16,17,18,19. During biomass burning events, analysis of the particles showed that metals such as Fe coexist with organic compounds20. Also, processing of mineral dust particles takes place in the atmosphere through reactions with acidic gases, organics, in the dark or under irradiation, changing the hygroscopicity of these particles and increasing the amount of dissolved Fe. The concentrations of Fe in raindrops, fog, and cloud waters range from 0.1 to 1138 × 10−6 mol L−1 21 depending on the location with typical concentration in cloud droplets of about 10−6 mol L−1 21. These processed Fe-containing particles can act as a sink for semivolatile organics from biomass burning and undergo mixing with biomass burning organic aerosol. The role of Fe in the aqueous phase formation of water-soluble and insoluble secondary light-absorbing organic products was reported in a number of lab studies using standard organic compounds22,23,24 or acidic extracts of biomass burning organic aerosol24. These studies highlighted the effect of Fe-catalyzed reactions on the morphology, hygroscopic, and ice nucleation properties of insoluble products and standard dust samples.
The objective of this investigation is to identify gas phase reaction products from the heterogenous reaction of gas phase NO2 with GA solutions containing nitrate and Fe ions, in the dark and under irradiation (300 nm < λ < 700 nm). We chose GA as a polyphenolic model of HULIS because the chemical structure of GA (Fig. 1, pKa1 = 4.16, pKa2 = 8.55, pKa3 = 11.4, pKa4 = 12.8)25 has the functional groups found in real HULIS26 and is more representative than the other aromatic proxy compounds such as catechol, syringol, guaiacol, gentisic acid, and resorcinol shown in Fig. 127,28,29,30. Nitrate (NO3−) is the most dominant water-soluble ion in the pH from 3 to 631, and Fe(III) is the most ubiquitous metal in the cloud, rain, fog with the pH ranging from 2 to 7 under oxic conditions21. Hence, aqueous solutions consisting of GA, GA/NO3−, GA/Fe(III), and GA/NO3−/Fe(III) were prepared as described in the methods section.
Real-time measurements of the gas phase products were performed using secondary electrospray ionization ultrahigh-resolution mass spectrometry (SESI-UHR-MS)32,33,34 under positive and negative ionization modes. The technique we used is state of the art mass spectrometry that provides qualitative information on the products (masses and hence structural information) and quantitative information from the relative intensities. The results indicate that the presence of NO3− and Fe(III) leads to an enhanced number of product compounds formed during the NO2 reactions with GA, especially under dark conditions. An important fraction of N-containing organic compounds were detected in the presence of NO3− and Fe(III). Intriguingly, the increase of the amount of NO3− in the presence of Fe(III) led to reduced number of N-containing product compounds. Majority of the produced N-containing compounds have reduced N-functional groups such as the polyamine compounds (putrescine), and aromatic amines containing two phenyl substituents, such as 4-dimethylaminobenzaldehyde, which could be toxic compounds.
Results and discussion
Characterization of the gas-phase product compounds
The formation of gas phase products was directly monitored using SESI-UHR-MS at a time resolution of 1 s. The data of gas products are shown in the Supplementary Data35. Figure 2A shows a hierarchical cluster analysis of 765 ions with intensities above 1 × 105 au captured in real-time measurements upon heterogeneous reaction of gaseous NO2 with aqueous GA/NO3−/Fe(III) in the dark. After removing the isotope peaks and conducting a statistical significance analysis test (two-tailed test, p < 0.0001), 53 ions shown in the group marked with a red rectangle in Fig. 2A were distinguished from all the monitored gas-phase compounds. The ions in the red rectangle exhibited an increasing intensity trend as the solution containing GA/NO3−/Fe(III) was exposed to gas phase NO2 in dark, which suggests that the detected ions are compounds formed during the NO2 reaction.
A Hierarchical cluster analysis of 765 ions detected in ESI+ mode upon heterogeneous reaction of NO2 with GA/Fe(III)/NO3− ([GA] = 1 × 10−6 mol L−1, [NO3−] = 0.05 mol L−1, [Fe(III)] = 1 × 10−6 mol L−1) in the dark. The normalized signal intensity in the hierarchical cluster is presented by a color-coded scale; i.e., the signal intensity increases from dark blue (normalized value of −3) to wine red (normalized value of 3). B Paired sample t test showing a significant difference (****p < 0.0001) in the concentration of each ion at the NO2 stage and zero-air stage in the dark. C A mass spectrum of the observed ions in ESI+ mode. Different formula groups are color-coded. The pie chart shows the proportion of CHO+, CHON+, CHN+, and unassigned peaks among all observed product compounds in the dark. D Real-time formation profiles of four product compounds that have been detected during the heterogeneous reaction of NO2 with GA/NO3−/Fe(III) in the dark. All of the data displayed here correspond to the products detected in ESI+ mode.
Figure 2B shows the results of the two-tailed test of m/z at 91.9981, 103.0387, 117.0544, and 131.0702. These results highlight statistically significant difference between the intensities at the zero-air stage and during the NO2 flow into the GA/NO3−/Fe(III) solution indicating that these ions are product compounds. Figure 2C shows the average mass spectra of the detected gas phase compounds, which include the relative number abundance. The intensities of the ions in the measured samples were normalized to the most abundant peak C7H3O5N (m/z 182.0090) ion as a reference compound (100%). The identified formulas are classified into three major compound categories as CHO+, CHON+, CHN+ according to the formula identification by means of Xcalibur software. Out of the 53 ions detected, the most abundant are CHON+ (49.1%) followed by CHO+ (36.8%), CHN+ (7%), and 7% are unassigned compounds. Figure 2D shows temporal resolution of m/z at 91.9981, 103.0387, 117.0544, and 131.0702 formed upon reaction of NO2 with GA in the presence of NO3− and Fe(III).
The influence of NO3− and Fe(III) on the formed gas phase product compounds during the heterogeneous reaction of NO2 with GA shown in Fig. 2 can be better explained by the Van Krevelen (VK) plots depicted in Fig. 3. Namely, the number of oxidized product compounds largely increased upon the addition of [NO3−] = 0.05 mol L−1 and [Fe(III)] = 1 × 10−6 mol L−1 especially during the reaction of NO2 with GA in dark (Fig. 3C, D).
Intriguingly, data in Supplementary Fig. S1 show that the increase of the amount of nitrate ions from 0.05 to 0.5 mol L−1 led to reduced number of the formed gas phase oxidized compounds during the reaction of NO2 with GA/Fe(III) in dark, while there is no significant difference under light irradiation.
Figure 3C shows that a majority of the gas phase product compounds are located in the region with 1 < H/C < 2 and O/C < 1, which is consistent with compounds identified in ambient aerosols (Supplementary Table S1)33,36. Figure 2C also shows that CHO compounds (44.7%) depicted in the range between H/C ≥ 1.5 and O/C ≤ 0.75 are most likely aliphatic compounds (alkanes, alkenes, and cyclic compounds)36,37,38. The compounds with Double Bond Equivalency (DBE) values ranging from 6 to 11 located in the domain with H/C ratio ≤1 and O/C ratio ≤0.5 in Fig. 2C indicate that they are polyaromatic compounds and unsaturated aromatic compounds, respectively. The compounds shown in Fig. 3C are classified in 6 CHO series compounds (Supplementary Fig. S2), exhibiting the same homologous CH2 series. For example, Supplementary Fig. S2 shows the series of C8H18O3(CH)2, C5H8O3(CH)2, C7H12O2(CH)2, C10H18O4(CH)2, with DBE < 2 and XC < 2.5, which could be the aliphatic compounds36. The series of C10H10O4(CH)2 and C8H4O3(CH)2, exhibiting the range in DBE values from 6 to 7 and the 2.5 < XC < 2.7, are monoaromatic compounds (Supplementary Fig. S2)36.
In addition to the formed CHO compounds, a large fraction of N-containing compounds (55.3%) were formed during the heterogeneous reaction of NO2 with GA in the presence of NO3− ([NO3−] = 0.05 mol L−1) and Fe(III) in the dark (Supplementary Table S1). Supplementary Tables S2–S12 show the products compounds detected under different conditions applied in this study. Figure 4 shows a VK plot obtained for the N-containing compounds in the form of H/C versus N/C, illustrating different ions with the same homologous CH2 series. The upper left corner of Fig. 4 in the range between H/C > 2 and N/C < 0.4 shows series of C7H15ON(CH)2 compounds with one N atom and DBE = 1, which are associated with aliphatic amines39. The series of C12H14O2N2(CH)2, C12H15N(CH)2, and C12H13O2N(CH)2 have DBE values ranging from 6 to 11, implying the presence of N-heterocyclic compounds with condensed aromatic rings (Fig. 4)40. The VK plot in Fig. 4 for H/C versus N/C shows that there are four CHN compounds with the DBE values ranging from 5 to 8. Three of the CHN compounds, with DBE values from 5 to 6 and XC > 2.6, are monoaromatic compounds (2.5 ≤ XC < 2.7) and one is polyaromatic compound (XC > 2.7) with DBE = 8 (Fig. 4 and Supplementary Table S1). The CHN compounds with six-membered rings are N-cyclohexylaniline (C12H17N), n-phenylcyclohexaneimine (C12H15N), and diphenylamine (C12H11N).
Moreover, gas phase compounds detected in the ESI+ ion mode are divided into different subgroups according to the O/N ratios. Figure 5 shows the contribution of each subgroup to the total intensity of CHON compounds in ESI+ ion mode. About 96% of the detected N-containing compounds exhibit O/N ratio ≤2. This result is reasonable considering that these compounds have reduced N-containing functional groups (e.g., amines, imines, and imides), which are easily protonated in ESI+ ion mode41.
Intriguingly, a majority (48%) of CHON compounds have reduced N-containing functional groups with O/N ratio of 1, such as C6H11ON, and heptanamide (C7H15ON), which are consistent with compounds identified in ambient aerosols42. CHON compounds with O/N ratio of 0.5 indicate the presence of a polyamine (1,4-diaminobutane) (C6H14ON2), which was also detected in atmospheric aerosols43. The gas phase compounds with C=N bond such as 1,4-diaminobutane (putrescine) and aromatic amines with two phenyl substituents, (4-dimethylaminobenzaldehyde), could be toxic compounds44. Another interesting subgroup is the one with O/N ratio of 0, which accounts for 16% of the total number of the detected N-containing organic compounds41. The compounds in this subgroup (O/N = 0) include C12H17N, C13H17N, and C12H11N, which have been also detected in ambient aerosols36.
It has to be noted that compounds with O/N ≤ 3 refer to reduced N-containing functional groups with C=N and C−N groups in their structure. These bonds were identified as the two most important chemical bonds of BrC in aerosol samples collected in Shanghai45. Examples of these compounds of atmospheric relevance include C22H43NO, C14H12N4O4, C16H15N3O5, C24H41NO, C27H41NO, and C35H29N5O245. Supplementary Figs. S3, S4, and S5 show that a small fraction of the N-containing product compounds contains oxidized nitrogen (CH3O4N, and C10H4O8N2) as detected in ESI+ mode. These compounds with XC > 2.5 exhibit O/N = 4 indicating they are aromatic compounds which contain nitrooxy (−ONO2) group; hence, they could be light-absorbing compounds associated with BrC in the atmosphere36,46. It has to be noted, however, that the same chemical formula does not necessarily imply that it is the same molecular structure as number of structural isomers are plausible for each formula47.
Role of the nitrate ion
The NO3− ion was added to the GA solutions prepared here to adjust the ionic strength at two concentrations, 0.05 and 0.5 mol L−1, which are 500–5000× higher than GA concentration. The ionic strength can affect the kinetics and product distribution within the aerosol deliquescent particles48,49. It has been shown that the addition of NO3− substantially favors the formation of polyaromatic compounds and substances with nitrooxy or oxygenated nitrooxy group of organonitrates through heterogeneous ozone processing of methoxyphenols46. Loisel et al.50 have shown that the photolytic degradation of vanillin in the presence of NO3− in the aerosol liquid water leads to the formation of condensed aromatic N-containing compounds compared to the corresponding degradation in the dilute aqueous phase of cloud droplets, which resulted in the formation of aliphatic-like CHO compounds. Degradation rates of syringaldehyde and acetosyringone in aqueous solutions containing NO3− were 1.5 and 3.5× faster than corresponding degradation rates in sulfate solutions51. It seemed that NO3− promoted the degradation of syringaldehyde and acetosyringone because nitrate ions are a photolytic source of OH radicals, while sulfate is not, highlighting the importance of NO3− in the formation of SOA by facilitating the photolytic degradation of organic compounds51.
In the dark, and under the experimental conditions described here, spontaneous redox reactions can take place driven by two-electron transfer between NO3− and GA. This is because the electrochemical reduction of NO3− to HNO2, N2O4, and NO2− under acidic to neutral conditions requires two electrons52 according to the following Equations:
The electrochemical oxidation of GA was studied as a function of pH and found to occur via an irreversible oxidation of GA to the semiquinone radical cation (GA•+) by an irreversible one-electron transfer process53. Then, the formed radical cation loses a proton to form the semiquinone radical (GA•) followed by a second irreversible one-electron transfer to the quinone cation (GA+). Finally, deprotonation of GA+ to give the quinone (GAO) completes the overall two-electron process53. At pH 5, the oxidation potentials of the first and second electron transfer steps were calculated to be 0.445 and 0.741 V53. Both of these oxidation potentials are lower than the reduction potentials of NO3− in Eqs. ((1)–(3)), which results in a net positive electrochemical cell potential, hence thermodynamically favorable redox paired reactions. In addition, data in Figs. 1C and S1 show that increasing nitrate concentrations under dark conditions significantly alter the number of gaseous products. Since GA is the limiting reagent in these redox paired reactions, it is very likely that the increase in ionic strength from increasing the nitrate concentration under dark conditions dramatically reduces the kinetics of subsequent oxidation pathways of GA following its initial oxidation to quinones. As a results, a limited number of products with XC < 2.5 are observed at [NO3−] = 0.5 M compared to the larger number of products with 2.5 ≤ XC < 2.71 and XC > 2.71 at [NO3−] = 0.05 M.
Under light irradiation, the absorption spectrum of NO3− is dominant by a weak n → π* band at 302 nm (ɛ = 7.2 M−1 cm−1) implying that nitrate ions can be photolyzed upon light irradiation of aqueous solutions containing nitrate ions producing oxidants such as hydroxyl radical (OH) and NO2, and nitrite ions (NO2−) as shown below54,55,56,57,58:
Considering that pKa1 of GA is 4.1625, a fraction of the compound is dissociated, at pH 5, implying fast photodegradation59. As a phenolic acid, GA is a known powerful natural antioxidant60. Its relatively low oxidation potential under acidic to neutral pH with three hydroxyl substituents on the benzene ring explain its strong free radical scavenging activity61. It has been shown that the reaction of GA with OH proceeds very fast with the rate constant (1.1 ± 0.1) × 1010 M−1 s−1 at pH 6.859,62,63, which is close to the diffusion limit64. The reaction of OH with GA at pH 6.8 leads to the formation of an adduct (GA-OH) which further reacts with GA yielding phenoxyl type radicals63.
It has been shown that the reaction of NO2 with HULIS can possibly lead to the formation of nitrous acid (HONO)13. For example, the heterogeneous reaction of NO2 with dry humic acid (prepared in aqueous solution, 1 mg mL−1, pH = 4.4) under light irradiation produced high amounts of gaseous HONO13. However, it has been shown that HONO cannot be detected in the gas phase during the heterogeneous reaction of NO2 with resorcinol used as a proxy of HULIS, at pH 6 or above, because all nitrite ions (NO2−) remain in the liquid film, which is reasonable considering that the pKa for HONO is 3.530. In our studies, the initial pH value of 5 increased in the course of the reaction to 11 (Supplementary Table S13), indicating that it is unlikely that HONO could be formed during the reaction of NO2 with GA. Nevertheless, we have observed a small amount of HONO in the gas phase ranging between 0.3 and 0.5 ppb during the reaction of NO2 with GA in presence of NO3− ([NO3−] = 0.05 mol L−1) in dark and under irradiation, respectively (Supplementary Fig. S6).
Role of Fe(III)
Considering that GA exists in excess of Fe(III) by a factor of 100, implies that Fe(III) will be complexed by three GA molecules in the dark, which will suppress the oxidation of GA in dark with and without nitrate ions because GA3Fe complexes are stable65. Under irradiation, and in the absence of nitrate, which means no OH radicals, the known reactions involve Fe(III) through the formation of a phenoxy radical intermediate, followed by reaction with OH + NO2, are described by the following set of Eqs. ((6)–(10))27,66:
Light absorption by [Fe(III)-GA]2+ leads to the formation of Fe(II) and phenoxy radicals (GA·) Eq. (6)27,67, followed by the reaction of phenoxy radicals with NO2 to yield the recombination products ((7) to (10))66.
The influence of Fe(III) on the HONO formation upon reaction of NO2 with GA in dark and under light irradiation is shown in Supplementary Figs. S7, S8. In the dark, about 0.3 ppb of HONO is formed, which increases up to 0.7 ppb under irradiation. The conversion of NO2 to HONO could be facilitated by Fe(III)15. Also, the complexed GA-Fe(III) complexes would be capable of protonating nitrite via hydrogen abstraction by NO2 due to the presence of hydroxyl groups on the benzene ring of GA and hydroxyl ligands on complexed Fe(III) to GA. Therefore, although the GA is responsible for reducing NO2 to NO2−, the presence of Fe(III) can facilitate the protonation of NO2 and the release of gaseous HONO even at pH 5 that is higher than the pKa of HONO (pKa = 2.8)68. The amount of HONO formed from aqueous GA mixed with Fe(III) shown in Supplementary Fig. S7 is slightly higher than that mixed with NO3− (Supplementary Fig. S6), indicating that indeed Fe(III) ions are capable of protonating nitrite.
Under irradiation, the HONO enhancement compared to dark observed in Supplementary Fig. S7 could be ascribed to photoexcitation of GA, followed by heterogeneous reduction of NO2 to HONO13,69,70,71. Substrates like GA that act as photosensitizing or photoreducing agents demonstrate this effect to the greatest extent69. This reduction has been proven to occur on organic substrates including humic substances, soil13, and urban grime72,73, at wavelengths not only in the UV-A spectral region, but also in the visible region under atmospheric conditions13. It has been shown that NO2 may be more efficiently hydrolyzed in aqueous solution when it is generated in situ during nitrate photolysis than during the heterogeneous reaction where mass transfer of gaseous NO2 into bulk solution is too slow71.
Benzoquinones are photoactive, which can generate semiquinone radicals when excited by light and in the presence of H-donors74. Therefore, it is reasonable to hypothesize that GA can reduce NO2 by the quinones or semiquinone species generated by Fe(III) chemistry in the dark or during light irradiation (Supplementary Fig. S11), followed by the conversion of NO2 to HONO in dark as well as during the irradiation.
The amounts of HONO are consistent with the mechanism, where the reduced species such as hydroquinone and semiquinones are generated when the quinones (forming from GA oxidation) are excited by light (Supplementary Fig. S12). Following their reaction with NO2, the phenols are reoxidized to the quinone state75, which can be cycled back to a phenol upon absorption of a photon or a chemical reductant. This mechanism accounts for difference in reactivity shown in Supplementary Fig. S11 and Supplementary Fig. S12. The efficiency of the reaction between NO2 and GA in dark decreases over time as semiquinone and hydroquinone reactive sites are oxidized to quinones (see Supplementary Fig. S11)68. In contrast, the photochemical reactivity observed in Supplementary Fig. S12 is relatively constant over the course of the experiment because photochemistry provides a mechanism which allows to regenerate semiquinones and hydroquinone reactive sites68.
The N-containing compounds formed upon heterogeneous reaction of NO2 with GA in the presence of Fe(III) in dark can be tentatively explained by formation of GA3Fe complexes (A1)65, which reacts with NO2 to generate Fe(II) and phenoxy radical (A2). A2 obtains an electron and an H atom to form phenoxyl radicals (A3)68, which can abstract H atom from hydroxy group of GA (Supplementary Fig. S13). Then A3 undergoes nucleophilic substitution reaction in the presence of NO3− to yield 3,4-dihydroxy-5-nitrobenzoic acid (C7H5O6N) (A4)46,76,77. A4 can be reduced in the presence of Fe(III) to 5-amino-3,4-hydroxybenzoic acid (C7H7O4N) (A5)78,79,80. A5 can abstract H atom from hydroxy group to form a radical, followed by the nucleophilic substitution reaction to generate 4-hydroxy-5-amino-3-nitrobenzoic acid (C7H6O5N2) (A7)46,76,77,81. A7 can be reduced in the presence of Fe(III) to 4-hydroxy-2,5-diaminobenzoic acid (C7H8O3N2) (A8)78,79,80. The A8 could abstract H atom from hydroxy group by NO2 radical to yield 2,5-diamino-1,4-benzoquinone (C6H6O2N2) (A9)82. Finally, the A9 could be oxidized by OH radical to form (C4H6O3N2) (A10)83, which undergoes decarboxylation leading to putrescine (C4H12N2) (A11)84 (Supplementary Fig. S13).
Conclusions
We showed that NO2 heterogeneous (photo)reactions of GA used as a proxy of HULIS in the presence of NO3− and Fe(III) ions can lead to the formation of a broad range of gas phase unsaturated and saturated organic compounds under atmospherically-relevant conditions. These results suggest that heterogeneous uptake of gaseous NO2 to wet aerosols containing HULIS would produce relatively higher amounts of VOCs than in the cloud droplets, considering that the ionic strength in deliquescent aerosol particles is much higher compared to the one in dilute aqueous phase in rain, fog, and cloud waters48,85. The majority of the formed compounds contain N atom in their structure, which can have a detrimental impact on human health upon inhalation and the environment given their toxicity and unique chemical reactivity. For example, the polyamine compounds, such as 1,4-diaminobutane (putrescine), and aromatic amines containing phenyl substituents, such as 4-dimethylaminobenzaldehyde, are potentially toxic compounds44,86. The secondarily formed amines, can be further nitrosated in the atmosphere generating N-nitroso compounds such as nitrosamines known for their adverse health effects87. In addition, the N-containing VOCs formed through the NO2 reactions with HULIS investigated here can have an additional contribution to the formation of light-absorbing SOA88,89.
This study forms the basis for future experimental work designed to obtain additional quantitative information such as absolute amounts of selected products and kinetics of reaction steps in the mechanism under the experimental conditions reported here. While bubbling NO2 gas into an aqueous phase solution followed by analyzing gas phase products is not identical to flowing NO2 over an aerosol particle or a suspended microdroplet, these experimental setups share the same elementary steps in the reaction mechanism such as adsorption, surface diffusion, accommodation to the bulk, and bulk diffusion90. Hence, results from the experimental setup described here provide a reference point and a bench mark for future work that aims to examine the roles of surface to volume ratio, particle curvature, and aerosol liquid water acidity and ionic strength.
Methods
Chemicals
Gallic acid (GA) (≥98.0%, CAS: 149-91-7, Coolaber), iron(III) chloride hexahydrate (FeCl3·6H2O) (≥98.0%, CAS: 10025-77-1, Aladdin), nitrate (NaNO3) (≥99.0%, CAS: 14797-55-8, Sigma Aldrich), sodium hydroxide (NaOH) (≥96.0%, CAS: 1310-73-2, Aladdin), hydrochloric acid (HCl) (ACS reagent grade, 99%, CAS: 7647-01-0, Guangzhou Chemical Reagent).
Experimental conditions
A double-well rectangular (5 cm × 5 cm × 2 cm) reactor made up of borosilicate glass was used to evaluate the formation of condensed phase products compounds and compounds released in the gas phase in dark and under light irradiation (Supplementary Fig. S9)32. A mixing ratio of 50 ppb obtained by diluting a pure NO2 flow (4.1 mL min−1) (0–10 mL min−1 mass flow controller, Seven Star, China) from a certified NO2 bottle gas of 10 ppm with air flow (800 mL min−1) (0–1000 mL min−1, Seven Star, China) was continuously passing through the reactor during the experiments. NO2 concentrations were measured online at the exit of the flow tube using an NO2 analyzer (Thermo Scientific, model 42i). The detection limit of the NOx analyzer was 300 ppt with a time resolution of 30 s. The reactor was filled with 10 mL of freshly prepared solution and exposed to NO2 in dark and light irradiation by a xenon lamp (Xe, 500 W, 300 nm < λ < 700 nm). The temperature in the flow tube reactor was held constant at 293 ± 0.02 K by water circulating through its jacket, which was connected with a thermostated bath (Lauda RC, Germany). Aqueous solutions consisting of GA ([GA] = 1 × 10−4 mol L−1), or of GA mixed with FeCl3·6H2O ([Fe(III) = 1 × 10 −6 mol L−1]), or of GA mixed NaNO3 ([NO3−] = 0.05, 0.5 mol L−1) or of GA mixed with FeCl3·6H2O and NaNO3 ([NO3−] = 0.05, 0.5 mol L−1), were prepared with ultra-pure water (Sartorius 18 MΩ cm, H2O-MM-UV-T, Germany). The pH values of these solutions were monitored by a pH meter (Mettler Toledo). To simulate the pH of clouds, fog and moderately acidic particles, pH was adjusted to 5 using sodium hydroxide (NaOH) ([NaOH] = 1 mol L−1) and hydrochloric acid (HCl) ([HCl] = 0.1 mol L−1) diluted from 37% v/v (ACS reagent grade).
Sample analysis using SESI-UHR-MS
The real-time measurements of gas phase compounds were performed by a SESI-UHR-MS (FIT, Spain; Q-Exactive, Thermo Scientific, USA) in both negative (ESI-) and positive (ESI+) ionization modes. In addition, blank measurements were performed before and after analysis. A SESI-UHR-MS and an NO2 analyzer were coupled to the double-wall rectangular reactor to observe the formed gas-phase products and the NO2, respectively, during the whole measurement period. The mass analyses ranged from 50 to 500 m/z, with a resolution above 1 × 105 arbitrary units (au) and a mass accuracy ≤2 ppm32,34. Regarding the experiments performed in dark and under light irradiation, in the first 15 min, the GA solution, in the absence or presence of NO3− and Fe(III), was exposed to a stream of air (800 mL min−1), followed by a 30 min exposure to a stream of 50 ppb of NO2 (800 mL min−1), then again exposed to a stream of air for 15 min. The spectral irradiance of the Xe lamp was measured with a calibrated spectroradiometer (Ocean Optics) equipped with a linear-array CCD detector (Supplementary Fig. S10).
Estimations of the DBE and aromaticity equivalent (X C) parameters
The product compounds were further visualized by multiple structure-dependent tools to improve their identification and characterization37 such as aromaticity equivalence (XC), Kendrick mass defect (KMD) analyses, and Van Krevelen (VK) plots. The degree of unsaturation of organic compounds is described by the oxygen-to-carbon ratio (O/C) versus the hydrogen-to-carbon ratio (H/C) in Van Krevelen plot40. Here, we calculated the KMD values by normalizing the CH2 group for CHO and CHON compounds according to the following Equations:
The double bond equivalent (DBE) for elemental composition CcHhOoNn can be expressed as follows:
The value of XC, identifying the aromatic and condensed aromatic structures in a complex mixture of compounds, was expressed as follows:
If \({DBE}\le m\times o\) or \({X}_{c}\le 0\), then \({X}_{c}=0\), where m is the fraction of O atoms in the π-bond structure of the compound. We used \(m=0.5\) for the calculation of XC in this study37. Threshold values of XC between 2.5 and 2.7 (2.5 ≤ XC < 2.7), indicating the presence of monoaromatic compounds, and equal or greater than 2.7 (XC ≥ 2.7), suggesting the presence of polyaromatic compounds, were set as minimum criteria for the presence of aromatic or condensed aromatic compounds in the identified ions37. In our calculations, the values of m and n were set to 0.5 since the negative mode of ESI is most sensitive to compounds that contain carboxylic functional groups and nitro compounds37,38.
Long-path absorption photometer (LOPAP)
The mixing ratio of HONO, produced by the heterogeneous reaction of NO2 with TA, TA/FeCl3·6H2O, TA/FeCl3·6H2O/NaNO3, GA, GA/FeCl3·6H2O, GA/FeCl3·6H2O/NaNO3, was online monitored in a long-path absorption cell after conversion into an azodye, absorbing at 550 nm. The long-path absorption cell is made out of Teflon tubing (Teflon AF2400), allowing light to be transferred in total reflection due to the low refractive index of the Teflon tubing. The detection limit was smaller than 3 ppt with a total accuracy of ±10% with an actual time response of about 5 min and a time resolution of 15 s.
Data availability
Datasets for the research are available in Supplementary Data35, and other data are available from the corresponding authors upon reasonable request.
References
Nakayama, T. et al. Wavelength and NOx dependent complex refractive index of SOAs generated from the photooxidation of toluene. Atmos. Chem. Phys. 13, 531–545 (2013).
He, Q. et al. Optical properties of secondary organic aerosol produced by nitrate radical oxidation of biogenic volatile organic compounds. Environ. Sci. Technol. 55, 2878–2889 (2021).
Kuang, B. Y., Lin, P., Huang, X. H. H. & Yu, J. Z. Sources of humic-like substances in the Pearl River Delta, China: positive matrix factorization analysis of PM2.5 major components and source markers. Atmos. Chem. Phys. 15, 1995–2008 (2015).
Krivácsy, Z. et al. Study of water-soluble atmospheric humic matter in urban and marine environments. Atmos. Res. 87, 1–12 (2008).
Mayol-Bracero, O. L. et al. Water-soluble organic compounds in biomass burning aerosols over Amazonia 2. Apportionment of the chemical composition and importance of the polyacidic fraction. J. Geophys. Res. Atmos. 107, LBA 59-51–LBA 59-15 (2002).
Laskin, A., Laskin, J. & Nizkorodov, S. A. Chemistry of atmospheric brown carbon. Chem. Rev. 115, 4335–4382 (2015).
Brown, H. et al. Biomass burning aerosols in most climate models are too absorbing. Nat. Commun. 12, 277 (2021).
Seinfeld, J. H. et al. Improving our fundamental understanding of the role of aerosol–cloud interactions in the climate system. Proc. Natl Acad. Sci. USA 113, 5781–5790 (2016).
Al-Abadleh, H. A. & Nizkorodov, S. A. Open questions on transition metals driving secondary thermal processes in atmospheric aerosols. Commun. Chem. 4, 176 (2021).
Finewax, Z., de Gouw, J. A. & Ziemann, P. J. Identification and quantification of 4-nitrocatechol formed from OH and NO3 radical-initiated reactions of catechol in air in the presence of NOx: implications for secondary organic aerosol formation from biomass burning. Environ. Sci. Technol. 52, 1981–1989 (2018).
Salvador, C. M. G. et al. Ambient nitro-aromatic compounds – biomass burning versus secondary formation in rural China. Atmos. Chem. Phys. 21, 1389–1406 (2021).
Mayorga, R. J., Zhao, Z. & Zhang, H. Formation of secondary organic aerosol from nitrate radical oxidation of phenolic VOCs: Implications for nitration mechanisms and brown carbon formation. Atmos. Environ. 244, 117910 (2021).
Stemmler, K., Ammann, M., Donders, C., Kleffmann, J. & George, C. Photosensitized reduction of nitrogen dioxide on humic acid as a source of nitrous acid. Nature 440, 195–198 (2006).
Kleffmann, J. Daytime sources of nitrous acid (HONO) in the atmospheric boundary layer. ChemPhysChem 8, 1137–1144 (2007).
Gen, M., Zhang, R. & Chan, C. K. Nitrite/nitrous acid generation from the reaction of nitrate and Fe(II) promoted by photolysis of iron-organic complexes. Environ. Sci. Technol. 55, 15715–15723 (2021).
Maher, B. A. et al. Global connections between aeolian dust, climate and ocean biogeochemistry at the present day and at the last glacial maximum. Earth Sci. Rev. 99, 61–97 (2010).
Conway, T. M. & John, S. G. Quantification of dissolved iron sources to the North Atlantic Ocean. Nature 511, 212–215 (2014).
Al-Abadleh, H. A. Review of the bulk and surface chemistry of iron in atmospherically relevant systems containing humic-like substances. RSC Adv. 5, 45785–45811 (2015).
Shi, Z. et al. Impacts on iron solubility in the mineral dust by processes in the source region and the atmosphere: a review. Aeol. Res. 5, 21–42 (2012).
Schlosser, J. S. et al. Analysis of aerosol composition data for western United States wildfires between 2005 and 2015: Dust emissions, chloride depletion, and most enhanced aerosol constituents. J. Geophys. Res. Atmos. 122, 8951–8966 (2017).
Deguillaume, L. et al. Transition metals in atmospheric liquid phases: sources, reactivity, and sensitive parameters. Chem. Rev. 105, 3388–3431 (2005).
Al-Abadleh, H. A. Aging of atmospheric aerosols and the role of iron in catalyzing brown carbon formation. Environ. Sci.: Atmos. 1, 297–345 (2021).
Al-Abadleh, H. A. et al. Reactivity of aminophenols in forming nitrogen-containing brown carbon from iron-catalyzed reactions. Commun. Chem. 5, 112 (2022).
Hopstock, K. S., Carpenter, B. P., Patterson, J. P., Al-Abadleh, H. A. & Nizkorodov, S. A. Formation of insoluble brown carbon through iron-catalyzed reaction of biomass burning organics. Environ. Sci.: Atmos. 3, 207–220 (2023).
Powell, H. K. J. & Taylor, M. C. Interactions of iron(II) and iron(III) with gallic acid and its homologues: a potentiometric and spectrophotometric study. Aust. J. Chem. 35, 739–756 (1982).
Zheng, G., He, K., Duan, F., Cheng, Y. & Ma, Y. Measurement of humic-like substances in aerosols: a review. Environ. Pollut. 181, 301–314 (2013).
Wentworth, G. R. & Al-Abadleh, H. A. DRIFTS studies on the photosensitized transformation of gallic acid by iron(iii) chloride as a model for HULIS in atmospheric aerosols. Phys. Chem. Chem. Phys. 13, 6507–6516 (2011).
Ammann, M., Rossler, E., Strekowski, R. & George, C. Nitrogen dioxide multiphase chemistry: uptake kinetics on aqueous solutions containing phenolic compounds. Phys. Chem. Chem. Phys. 7, 2513–2518 (2005).
Sosedova, Y., Rouvière, A., Bartels-Rausch, T. & Ammann, M. UVA/Vis-induced nitrous acid formation on polyphenolic films exposed to gaseous NO2. Photochem. Photobiol. Sci. 10, 1680–1690 (2011).
Gutzwiller, L., George, C., Rössler, E. & Ammann, M. Reaction kinetics of NO2 with resorcinol and 2,7-naphthalenediol in the aqueous phase at different pH. J. Phys. Chem. A 106, 12045–12050 (2002).
Shi, G. et al. pH of aerosols in a polluted atmosphere: source contributions to highly acidic aerosol. Environ. Sci. Technol. 51, 4289–4296 (2017).
Mekic, M. et al. Ionic strength effect on photochemistry of fluorene and dimethylsulfoxide at the air–sea interface: alternative formation pathway of organic sulfur compounds in a marine atmosphere. ACS Earth Space Chem. 4, 1029–1038 (2020).
Wang, Y. et al. Interfacial ozone oxidation chemistry at a riverine surface microlayer as a source of nitrogen organic compounds. Environ. Sci. Technol. Lett. 9, 493–500 (2022).
Deng, H. et al. Daytime SO2 chemistry on ubiquitous urban surfaces as a source of organic sulfur compounds in ambient air. Sci. Adv. 8, eabq6830–eabq6830 (2022).
Li, P. et al. Formation of nitrogen-containing gas phase products from the heterogeneous (photo)reaction of NO2 with gallic acid. Science Data Bank https://doi.org/10.57760/sciencedb.10450 (2023).
Wang, X. et al. Chemical characteristics of organic aerosols in shanghai: a study by ultrahigh-performance liquid chromatography coupled with orbitrap mass spectrometry. J. Geophys. Res. Atmos. 122, 11703–11722 (2017).
Yassine, M. M., Harir, M., Dabek-Zlotorzynska, E. & Schmitt-Kopplin, P. Structural characterization of organic aerosol using Fourier transform ion cyclotron resonance mass spectrometry: aromaticity equivalent approach. Rapid Commun. Mass Spectrom. 28, 2445–2454 (2014).
Kourtchev, I. et al. Molecular composition of organic aerosols in central Amazonia: an ultra-high-resolution mass spectrometry study. Atmos. Chem. Phys. 16, 11899–11913 (2016).
Lin, P., Rincon, A. G., Kalberer, M. & Yu, J. Z. Elemental composition of HULIS in the Pearl River Delta Region, China: results inferred from positive and negative electrospray high resolution mass spectrometric data. Environ. Sci. Technol. 46, 7454–7462 (2012).
Laskin, A., Smith, J. S. & Laskin, J. Molecular characterization of nitrogen-containing organic compounds in biomass burning aerosols using high-resolution mass spectrometry. Environ. Sci. Technol. 43, 3764–3771 (2009).
Wang, K., Zhang, Y., Huang, R., Cao, J. & Hoffmann, T. UHPLC-Orbitrap mass spectrometric characterization of organic aerosol from a central European city (Mainz, Germany) and a Chinese megacity (Beijing). Atmos. Environ. 189, 22–29 (2018).
Wang, M. et al. Chemical characteristics and sources of nitrogen-containing organic compounds at a regional site in the North China Plain during the transition period of autumn and winter. Sci. Total Environ. 812, 151451 (2022).
Titz, B. et al. Multi-omics systems toxicology study of mouse lung assessing the effects of aerosols from two heat-not-burn tobacco products and cigarette smoke. Comput. Struct. Biotechnol. J. 18, 1056–1073 (2020).
Til, H. P., Falke, H. E., Prinsen, M. K. & Willems, M. I. Acute and subacute toxicity of tyramine, spermidine, spermine, putrescine and cadaverine in rats. Food Chem. Toxicol. 35, 337–348 (1997).
Zeng, Y. et al. The roles of N, S, and O in molecular absorption features of brown carbon in PM2.5 in a typical semi-arid megacity in Northwestern China. J. Geophys. Res. Atmos. 126, e2021JD034791 (2021).
Wang, Y. et al. Ionic strength effect triggers brown carbon formation through heterogeneous ozone processing of ortho-vanillin. Environ. Sci. Technol. 55, 4553–4564 (2021).
Nizkorodov, S. A., Laskin, J. & Laskin, A. Molecular chemistry of organic aerosols through the application of high resolution mass spectrometry. Phys. Chem. Chem. Phys. 13, 3612–3629 (2011).
Mekic, M. & Gligorovski, S. Ionic strength effects on heterogeneous and multiphase chemistry: clouds versus aerosol particles. Atmos. Environ. 244, 117911 (2021).
Mekic, M., Wang, Y., Loisel, G., Vione, D. & Gligorovski, S. Ionic strength effect alters the heterogeneous ozone oxidation of methoxyphenols in going from cloud droplets to aerosol deliquescent particles. Environ. Sci. Technol. 54, 12898–12907 (2020).
Loisel, G. et al. Ionic strength effect on the formation of organonitrate compounds through photochemical degradation of vanillin in liquid water of aerosols. Atmos. Environ. 246, 118140 (2021).
Huang, D. D. et al. Formation and evolution of aqSOA from aqueous-phase reactions of phenolic carbonyls: comparison between ammonium sulfate and ammonium nitrate solutions. Environ. Sci. Technol. 52, 9215–9224 (2018).
Lide, D. R. CRC handbook of chemistry and physics. CRC Press 93, 2012–2013 (2012).
Abdel-Hamid, R. & Newair, E. F. Electrochemical behavior of antioxidants: I. Mechanistic study on electrochemical oxidation of gallic acid in aqueous solutions at glassy-carbon electrode. J. Electroanal. Chem. 657, 107–112 (2011).
Mack, J. & Bolton, J. R. Photochemistry of nitrite and nitrate in aqueous solution: a review. J. Photochem. Photobiol. A 128, 1–13 (1999).
Benedict, K. B., McFall, A. S. & Anastasio, C. Quantum yield of nitrite from the photolysis of aqueous nitrate above 300 nm. Environ. Sci. Technol. 51, 4387–4395 (2017).
Nissenson, P. et al. Evidence of the water-cage effect on the photolysis of NO3− and FeOH2+. Implications of this effect and of H2O2 surface accumulation on photochemistry at the air–water interface of atmospheric droplets. Atmos. Environ. 44, 4859–4866 (2010).
Gen, M., Liang, Z., Zhang, R., Go Mabato, B. R. & Chan, C. K. Particulate nitrate photolysis in the atmosphere. Environ. Sci.: Atmos. 2, 111–127 (2022).
Vione, D. et al. Sources and sinks of hydroxyl radicals upon irradiation of natural water samples. Environ. Sci. Technol. 40, 3775–3781 (2006).
Melo, R., Leal, J. P., Takacs, E. & Wojnarovits, L. Radiolytic degradation of gallic acid and its derivatives in aqueous solution. J. Hazard. Mater. 172, 1185–1192 (2009).
Chiorcea-Paquim, A. M., Enache, T. A., De Souza Gil, E. & Oliveira-Brett, A. M. Natural phenolic antioxidants electrochemistry: Towards a new food science methodology. Compr. Rev. Food Sci. Food Saf. 19, 1680–1726 (2020).
Marino, T., Galano, A. & Russo, N. Radical scavenging ability of gallic acid toward OH and OOH radicals. Reaction mechanism and rate constants from the density functional theory. J. Phys. Chem. B 118, 10380–10389 (2014).
Benitez, F. J., Real, F. J., Acero, J. L., Leal, A. I. & Garcia, C. Gallic acid degradation in aqueous solutions by UV/H2O2 treatment, Fenton’s reagent and the photo-Fenton system. J. Hazard. Mater. 126, 31–39 (2005).
Dwibedy, P., Dey, G. R., Naik, D. B., Kishore, K. & Moorthy, P. N. Pulse radiolysis studies on redox reactions of gallic acid: one electron oxidation of gallic acid by gallic acid OH adduct. Phys. Chem. Chem. Phys. 1, 1915–1918 (1999).
Gligorovski, S. & Herrmann, H. Kinetics of reactions of OH with organic carbonyl compounds in aqueous solution. Phys. Chem. Chem. Phys. 6, 4118–4126 (2004).
Fazary, A. E., Taha, M. & Ju, Y.-H. Iron complexation studies of gallic acid. J. Chem. Eng. Data 54, 35–42 (2009).
Harrison, M. A. J. et al. Nitrated phenols in the atmosphere: a review. Atmos. Environ. 39, 231–248 (2005).
Hynes, M. J. & Coinceanainn, M. O. The kinetics and mechanisms of the reaction of iron(III) with gallic acid, gallic acid methyl ester and catechin. J. Inorg. Biochem 85, 131–142 (2001).
Scharko, N. K., Martin, E. T., Losovyj, Y., Peters, D. G. & Raff, J. D. Evidence for quinone redox chemistry mediating daytime and nighttime NO2-to-HONO conversion on soil surfaces. Environ. Sci. Technol. 51, 9633–9643 (2017).
George, C., Strekowski, R. S., Kleffmann, J., Stemmler, K. & Ammann, M. Photoenhanced uptake of gaseous NO2 on solid organic compounds: a photochemical source of HONO? Faraday Discuss 130, 195–210 (2005).
Stemmler, K. et al. Light induced conversion of nitrogen dioxide into nitrous acid on submicron humic acid aerosol. Atmos. Chem. Phys. 7, 4237–4248 (2007).
Scharko, N. K., Berke, A. E. & Raff, J. D. Release of nitrous acid and nitrogen dioxide from nitrate photolysis in acidic aqueous solutions. Environ. Sci. Technol. 48, 11991–12001 (2014).
Liu, J. et al. Photoenhanced uptake of NO2 and HONO formation on real urban grime. Environ. Sci. Technol. Lett. 6, 413–417 (2019).
Liu, J. et al. Physical and chemical characterization of urban grime: an impact on the NO2 uptake coefficients and N-containing product compounds. Sci. Total Environ. 838, 155973 (2022).
Griesbeck, A., Oelgemöller, M. & Ghetti, F. CRC Handbook of Organic Photochemistry and Photobiology Third edition (CRC Press, 2012).
Woodill, L. A. & Hinrichs, R. Z. Heterogeneous reactions of surface-adsorbed catechol with nitrogen dioxide: substrate effects for tropospheric aerosol surrogates. Phys. Chem. Chem. Phys. 12, 10766–10774 (2010).
Deng, Y. et al. Iron-catalyzed photochemical transformation of benzoic acid in atmospheric liquids: product identification and reaction mechanisms. Atmos. Environ. 40, 3665–3676 (2006).
Hartshorn, M. P. et al. Reactions of substituted phenols with nitrogen dioxide. Rearrangements and addition reactions of nitrodienones. Acta Chem. Scand. 52, 2–10 (1998).
Luan, F., Xie, L., Li, J. & Zhou, Q. Abiotic reduction of nitroaromatic compounds by Fe(II) associated with iron oxides and humic acid. Chemosphere 91, 1035–1041 (2013).
Keum, Y. S. & Li, Q. X. Reduction of nitroaromatic pesticides with zero-valent iron. Chemosphere 54, 255–263 (2004).
Pan, Y. et al. Water vapor condensation on iron minerals spontaneously produces hydroxyl radical. Environ. Sci. Technol. 57, 8610–8616 (2023).
Li, P. et al. Inorganic ions enhance the number of product compounds through heterogeneous processing of gaseous NO2 on an aqueous layer of acetosyringone. Environ. Sci. Technol. 56, 5398–5408 (2022).
Beake, B. D., Constantine, J. & Moodie, R. B. Nitration and oxidation of 4-methoxyphenol by nitrous acid in aqueous acid solution. J. Chem. Soc., Perkin Trans. 2, 335–340, https://doi.org/10.1039/P29940000335 (1994).
Pan, X.-M., Schuchmann, M. N. & von Sonntag, C. Oxidation of benzene by the OH radical. A product and pulse radiolysis study in oxygenated aqueous solution. J. Chem. Soc., Perkin Trans. 2, 289–297, https://doi.org/10.1039/p29930000289 (1993).
Joanna Stadnik, Z. J. D. Biogenic amines in meat and fermented meat products. Acta Sci. Pol. Technol. Aliment. 9, 251–263 (2010).
Herrmann, H. Kinetics of aqueous phase reactions relevant for atmospheric chemistry. Chem. Rev. 103, 4691–4716 (2003).
Walker, J. D. & Printup, H. Structure–activity relationships for aldehyde categories. QSAR Comb. Sci. 27, 475–482 (2008).
Greim, H., Bury, D., Klimisch, H. J., Oeben-Negele, M. & Zeigler-Skylakakis, Z. Toxicity of aliphatic amines: structure-activity relationship. Chemosphere 36, 271–295 (1998).
Murphy, S. M. et al. Secondary aerosol formation from atmospheric reactions of aliphatic amines. Atmos. Chem. Phys. 7, 2313–2337 (2007).
Dawson, M. L. et al. Simplified mechanism for new particle formation from methanesulfonic acid, amines, and water via experiments and ab initio calculations. Proc. Natl Acad. Sci. USA 109, 18719–18724 (2012).
Ruiz-Lopez, M. F., Francisco, J. S., Martins-Costa, M. T. C. & Anglada, J. M. Molecular reactions at aqueous interfaces. Nat. Rev. Chem. 4, 459–475 (2020).
Acknowledgements
This work was financially supported by Ministry of Science and Technology of China (2022YFC3701102), the National Natural Science Foundation of China (42177087, 41977187, and 42207127), National Natural Science Foundation of China, Research Fund for International Scientists (4221101064), Chinese Academy of Science, International Cooperation Grant (132744KYSB20190007), State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry (SKLOG2020-5, and KTZ_17101), and Guangdong Foundation for Program of Science and Technology Research (2017B030314057), National Key R&D Program of China (2021YFC1808903), the Key-Area Research and Development Program of Guangdong Province (2019B110207002), Science and Technology Planning Project of Guangdong Province (200106105876892 and 190807115560881).
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Li, P., Gemayel, R., Li, X. et al. Formation of nitrogen-containing gas phase products from the heterogeneous (photo)reaction of NO2 with gallic acid. Commun Chem 6, 198 (2023). https://doi.org/10.1038/s42004-023-01003-3
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DOI: https://doi.org/10.1038/s42004-023-01003-3
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