Abstract
In this study, we aimed to evaluate the anti-inflammatory and anti-apoptotic effects of melatonin (MLT) on neuropathic pain (NP)-induced anxiety and depression in a rat model. Adult male rats were separated into four groups, i.e., Sham-VEH: healthy animals received a vehicle, Sham-MLT (10 mg/kg), and chronic constrictive injury (CCI)-VEH: nerve ligation received the vehicle, and CCI-MLT. Next, we used behavioral tests to evaluate pain severity, anxiety, and depression. Finally, rats were sacrificed for molecular and histopathological studies. Behavioral tests showed that NP could induce depressive- and anxiety-like behaviors. NP activated NF-κB/NLRP3 inflammasome pathways by upregulating NF-κB, NLRP3, ASC, active Caspase-1, also enhancing the concentrations of cytokines (IL-1β and IL-18) in the prefrontal cortex (PFC) and hippocampus (HC). NP upregulated Bax, downregulated Bcl2, and increased cell apoptosis in the HC and PFC. The rats treated with MLT eliminated the effects of NP, as the reduced pain severity, improved anxiety- and depressive-like behaviors, ameliorated NF-κB/NLRP3 inflammasome pathways, and modulated levels of cytokines in the HC and PFC. MLT could promote cell survival from apoptosis by modulating Bax and Bcl2. Therefore, it might be inferred that its anti-inflammatory and anti-apoptotic properties mediate the beneficial effects of MLT in NP-induced affective disorders.
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Introduction
Peripheral neuropathic pain (NP) is a chronic pain state with several complications. It is a result of primary damage or disease influencing the central nervous system (CNS)1. According to the study, it has affected about 20% of the total population and is accompanied by spontaneous pain, hyperalgesia, and mechanical allodynia2. Up to 80% of patients with chronic pain suffer from mental health disorders, e.g., depression and anxiety3,4,5. Patients with mental disorders may also experience unusual pain modulation and perception that enhance the risk of chronic pain establishment6.
The pathogenesis of NP-induced depressive-like behaviors and anxiolytic-like behaviors have not been fully understood. The prefrontal cortex (PFC) and hippocampus (HC) are key regions in pain processing. Both acute and chronic pain can alter the neurotransmitter content and cause gene dysregulation, glial cell dysfunction, and neuroinflammation in the HC and PFC7; consequently, they induce changes in the structure, function, and connectivity in these regions8,9. Pain hypersensitivity and maintenance of chronic pain are associated with glial-neuronal interactions10. Glial cells initiate multiple signaling events to modulate pain processing at spinal and supraspinal levels. They secrete inflammatory chemokines and cytokines to facilitate pain transmission11. Persistent neuroinflammation following chronic pain leads to several structural changes and subsequently induces mood swings12. Neuroinflammation is characterized by microglial/astrocytic activation, chemokine and cytokine production, and leukocyte activation and infiltration12. Increased levels of these inflammatory factors (e.g., chemokines and cytokines) are widely distributed through local tissues, peripheral nerves, dorsal root ganglia, and the CNS, thereby contributing to the initiation and maintenance of chronic pain13,14,15. NP-induced emotional disorders are associated with neuroinflammation in the PFC and HC16,17. High levels of pro-inflammatory cytokines, e.g., interleukin (IL)-1β, in the PFC and HC have been demonstrated in pain models8. Besides, the activation of pro-inflammatory mediators is associated with neuronal damage in animal models of depression18.
Current therapeutic approaches for the management of chronic pain, e.g., opioids and physical therapy, are often insufficient and inadequate19,20. Melatonin (MLT, N-acetyl-5-methoxytryptamine) is implicated in regulating sleep and circadian rhythms21. MLT with antioxidant and anti-inflammatory features can be used to prevent and treat neurological disorders22. Many of its properties are still under study, and its effectiveness in clinical trials is still unknown. MLT exhibits biological properties, including potential analgesic, antidepressant, anti-inflammatory, anti-apoptotic, and neuroprotective actions23,24,25. However, the therapeutic value of MLT and its associated mechanisms are still under investigation. The detailed characterization of the neuronal mechanisms underlying the antidepressant-like effects of MLT is yet to be studied, especially if it could elicit neuroprotective effects by suppressing the activity of inflammatory processes and apoptosis, further contributing to neuronal damage in patients with depression induced by chronic constriction injury (CCI).
In this preclinical study, the effects of MLT on pain-related behaviors and associated inflammatory factors in the CCI sciatic nerve model of chronic NP were assessed. The effects of MLT on the following factors were examined: (1) pain severity, (2) anxiety and depression, (3) activation of NF-κB/NLRP3 axis components (NF-κB, NLRP3, ASC, and Casp-1); secretion of cytokines (IL-1β and IL-18), and (4) apoptosis and apoptotic regulatory mediators (Bax and Bcl2) in the HC and PFC.
Results
MLT administration alleviated the pain behavior and improved anxiety- and depressive-like behaviors induced by CCI
To test whether MLT could impact CCI-induced pain behaviors, we examined the pain threshold (hot plate test [HPT] and an acetone drop test [ADT]) and evaluated anxiety- and depressive-like behaviors (forced swimming test [FST], elevated plus maze test [EPMT], tail suspension test [TST], and open field test [OFT]) of the rats.
HPT findings indicated that there was no significant difference in the heat pain threshold of different study groups on day 0. CCI induction significantly decreased the heat pain threshold of the CCI-VEH group on days 3, 7, 14, and 21 of evaluation compared to those of the Sham-VEH group and those of the CCI-VEH group on day 0 (Fig. 1B, P < 0.0001). Furthermore, the MLT treatment significantly increased the pain threshold compared with that of the CCI-VEH group on days 7 (Fig. 1B, P < 0.01), 14 (Fig. 1B, P < 0.01), and 21 (Fig. 1B, P < 0.001). Furthermore, the MLT treatment significantly enhanced the pain threshold of the CCI-MLT group on day 21 compared with that of the CCI-MLT group on days 3 (Fig. 1B, P < 0.05) and 7 (Fig. 1B, P < 0.05).
(A) Study protocol. Effects of melatonin (MLT) on the pain and flowing mood problems in the rat model of chronic constriction injury (CCI). (B) Heat hyperalgesia and (C) Cold allodynia for the assessment of pain on days 0, 3, 14, and 21, (D–F) forced swimming test, (G,H) elevated plus maze test, (I) tail suspension test, and (J,K) open field test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 compared with the Sham-VEH group; #P < 0.05, ##P < 0.01. ###P < 0.001, and ####P < 0.0001 compared with the Sham-MLT group, +P < 0.05, ++P < 0.01, +++P < 0.001, and ++++P < 0.0001 compared with the CCI-VEH group. aP < 0.05 compared with the same group on day 3, bP < 0.05 compared with the same group on day 7, cP < 0.05 compared with the same group on day 14, dP < 0.05 compared with the same group on day 21. Sham-VEH: sham-operated animals, Sham-MLT: sham-operated animals MLT (10 mg/kg), CCI-VEH: pain-induced animals, and CCI-MLT: pain-induced animals received MLT (10 mg/kg), mean ± standard deviation.
ADT findings also showed that no significant difference was observed in the allodynia scores of different study groups on day 0. Furthermore, CCI increased the cold allodynia scores of the CCI-VEH group on days 3 (Fig. 1C, P < 0.05), 7, 14 and 21 (Fig. 1C, P < 0.0001) compared with those of Sham-VEH group and those of the CCI-VEH group on day 0. The MLT treatment significantly reduced the allodynia scores compared with those of the CCI-VEH group on days 7 (Fig. 1C, P < 0.01), 14 (Fig 1C, P < 0.01), and 21 (Fig. 1C, P < 0.001). In addition, the MLT treatment significantly reduced the allodynia scores of the CCI-MLT group on day 21 compared with those of the CCI-MLT group on day 7 (Fig. 1B, P < 0.05).
The FST test demonstrated that the immobility time of the CCI-VEH group increased, but its swimming time and climbing time decreased compared with those of the Sham-VEH and Sham-MLT groups (Fig. 1D–F, P < 0.0001 and P < 0.0001, respectively). MLT administration significantly improved the altered behaviors in the CCI-MLT group compared with those of the CCI-VEH group (Fig. 1D–F, P < 0.0001). The EPMT findings showed that CCI appreciably decreased rat activity (duration) in the open and increased the duration in the closed arm of the CCI-VEH group compared with that of the Sham-VEH and Sham-MLT groups (Fig. 1G,H, P < 0.0001 and P < 0.0001, respectively). The EPMT values of the CCI-MLT group improved compared with that of the CCI-VEH group (Fig. 1G,H, P < 0.0001). Furthermore, the TST revealed that CCI increased the immobility time (Fig. 1I, P < 0.0001 and P < 0.0001, respectively) of the CCI-VEH group compared with that of the Sham-VEH and Sham-MLT groups. MLT noticeably improved the altered behaviors of the rats (Fig. 1I, P < 0.0001). Meanwhile, the OFT findings indicated that CCI palpably decreased the delay time in the central zone and prolonged the delay time in the peripheral zone of the CCI-VEH group compared with those of the Sham-VEH and Sham-MLT groups (Fig. 1J,K, P < 0.0001 and P < 0.0001, respectively). The OFT values of the CCI-MLT animals also were improved compared with those of the CCI-induced animals (Fig. 1J,K, P < 0.0001). Therefore, MLT administration alleviated CCI-induced pain behaviors and anxiety and depression symptoms.
MLT administration regulated the CCI-induced neuroinflammation in HC and PFC
To determine whether MLT regulated the CCI-induced neuroinflammation in the CNS associated with anxiety and depressive-like behaviors, we examined the inflammasomes in PFC and HC at gene and protein expression levels.
qRT-PCR showed that the gene expression levels of NF-κB, NLRP3, ASC, Casp-1, IL-1β, and IL-18 increased in the HC of the CCI-VEH group compared with those of the Sham-VEH and Sham-MLT groups (Fig. 2A–F, P < 0.0001 and P < 0.0001, respectively). MLT markedly downregulated the gene expression levels of NF-κB (Fig. 2A, P < 0.01), NLRP3, ASC, Casp-1, IL-1β, and IL-18 (Fig. 2B–F, P < 0.0001) in the HC compared with those of the CCI-VEH group. Moreover, the gene expression levels of NF-κB, NLRP3, ASC, Casp-1, IL-1β, and IL-18 increased in the PFC of the CCI-VEH group compared with those of the Sham-VEH and Sham-MLT groups (Fig. 2G–L, P < 0.0001 and P < 0.0001, respectively). MLT reduced the gene expression levels of NF-κB, NLRP3, ASC, Casp-1, IL-1β, and IL-18 in the PFC compared with those of the CCI-VEH rats (Fig. 2G–L, P < 0.0001).
Effects of melatonin (MLT) on the gene expression of (A,G) nuclear factor-κB, (B,H) NLRP3, (C,I) ASC, (D,J) Caspase-1, (E,K) IL-1β and (F,L) IL-18 in the hippocampus and prefrontal cortex of chronic constriction injury (CCI)-induced rats. *P < 0.05, ***P < 0.001, and ****P < 0.0001 compared with the Sham-VEH group; ##P < 0.01, ###P < 0.001, and ####P < 0.0001 compared with the Sham-MLT group, ++P < 0.01 and ++++P < 0.0001 compared with the CCI-VEH group. Sham-VEH: sham-operated animals, Sham-MLT: sham-operated animals MLT (10 mg/kg), CCI-VEH: pain-induced animals, and CCI-MLT: pain-induced animals received MLT (10 mg/kg), mean ± standard deviation, n = 6 per group.
ELISA findings showed that the protein levels of NF-κB, NLRP3, ASC, Casp-1, IL-1β, and IL-18 increased in the HC of the CCI-VEH group compared with those of the Sham-VEH and Sham-MLT groups (Fig. 3A–F, P < 0.0001 and P < 0.0001, respectively). MLT significantly reduced the protein levels of NF-κB, NLRP3, ASC, Casp-1, IL-1β, and IL-18 in the HC compared with that of the CCI-VEH group (Fig. 3A–F, P < 0.0001). Furthermore, the protein levels of NF-κB, NLRP3, ASC, Casp-1, IL-1β, and IL-18 increased in the PFC of the CCI-VEH group compared with that of the Sham-VEH and Sham-MLT groups (Fig. 3G–L, P < 0.0001 and P < 0.0001, respectively). MLT significantly reduced the protein levels of NF-κB, NLRP3, ASC, Casp-1, IL-1β, and IL-18 in the PFC compared with that of the CCI-VEH rats (Fig. 3G–L, P < 0.0001). Therefore, MLT administration could alleviate NP-induced neuroinflammation in the CNS at gene and protein levels.
Effects of melatonin (MLT) on the protein levels of (A,G) nuclear factor-κB, (B,H) NLRP3, (C,I) ASC, (D,J) Caspase-1, (E,K) IL-1β and (F,L) IL-18 in the hippocampus and prefrontal cortex of chronic constriction injury (CCI)-induced rats. ****P < 0.0001 compared with the Sham-VEH group; ####P < 0.0001 compared with the MLT group, and ++++P < 0.0001 compared with the CCI-VEH group. Sham-VEH: sham-operated animals, Sham-MLT: sham-operated animals MLT (10 mg/kg), CCI-VEH: pain-induced animals, and CCI-MLT: pain-induced animals received MLT (10 mg/kg), mean ± standard deviation, n = 6 per group.
MLT administration modulated the CCI-induced apoptosis and reduced dark neurons in HC and PFC
To investigate whether MLT could attenuate the rate of apoptosis, which is a severe consequence of neuroinflammation, we examined the protein levels of apoptotic factors and observed the morphological characteristics of neurons.
The protein levels of Bax significantly raised in the HC and PFC of the CCI-VEH group compared with those of the Sham-VEH and Sham-MLT groups (Fig. 4A,B,E,F, P < 0.0001 and P < 0.0001, respectively). MLT significantly decreased the protein levels of Bax in the HC and PFC (Fig. 4A,B,E,F, P < 0.0001) compared with those of the CCI-VEH group. Moreover, the protein levels of Bcl2 outstandingly reduced in the HC and PFC of the CCI-VEH group compared with those of the Sham-VEH and Sham-MLT groups (Fig. 4A,C,E,G, P < 0.0001 and P < 0.0001, respectively). MLT notably increased protein levels of Bcl2 in the HC and PFC compared with those of the CCI-VEH group (Fig. 4A,C,E,G, P < 0.001). The Bax/Bcl2 ratio increased in the HC and PFC of the CCI-VEH group compared with that of the Sham-VEH and Sham-MLT groups (Fig. 4D,H, P < 0.0001 and P < 0.0001, respectively). Conversely, MLT reduced the Bax/Bcl2 ratio in the HC and PFC of the CCI-MLT group compared with that of the CCI-VEH group (Fig. 4D,H, P < 0.0001). The original western blot images of Fig. 4A,E can be found in the figures S1 and S2 of the supplementary file.
Effects of melatonin (MLT) on Bax and Bcl2 concentrations in the hippocampus (HC) and prefrontal cortex (PFC) of chronic constriction injury (CCI)-induced rats. (A) Results of western blot assay, concentrations of (B) Bax and (C) Bcl2, (D) Bax-to-Bcl2 ratio in the HC, (E) results of Western blot assay, concentrations of (F) Bax and (G) Bcl2, (H) Bax-to-Bcl2 ratio in the PFC. ****P < 0.0001 compared with the Sham-VEH group; ####P < 0.0001 compared with the Sham-MLT group, and ++++P < 0.0001 compared with the CCI-VEH group. Sham-VEH: sham-operated animals, Sham-MLT: sham-operated animals MLT (10 mg/kg), CCI-VEH: pain-induced animals, and CCI-MLT: pain-induced animals received MLT (10 mg/kg), mean ± standard deviation, n = 6 per group. The original western blot images can be found in the figures S1 and S2 of the supplementary file. Images of blots with adequate length and membrane edges could not be provided because the blots were cut prior to hybridisation with antibodies and scanned at inside of blots, respectively.
The percentage of dark neurons in the PFC, CA1, CA3, and DG of the CCI-VEH group increased compared with that of the Sham-VEH and Sham-MLT groups (Fig. 5A–F, P < 0.0001 and P < 0.0001, respectively). MLT reduced the percentage of dark neurons in the PFC, CA1, CA3, and DG of the CCI-MLT group compared with that of the CCI-VEH group (Fig. 5A–F, P < 0.0001).
Effects of melatonin (MLT) on (A) the percentage of dark neurons in the hippocampus and prefrontal cortex (PFC) of chronic constriction injury (CCI)-induced rats. Nissl staining of (A) the PFC and (B) different parts of the HC (10× and 400×). Arrows show the basophilic and shrunken cells known as dark neurons. Comparing the percentage of dark neurons in the (C) PFC, (D) CA1, (E) CA3, and (F) DG among different groups. ****P < 0.0001 compared with the Sham-VEH group; ####P < 0.0001 compared with the Sham-MLT group, and ++++P < 0.0001 compared with the CCI-VEH group. Sham-VEH: sham-operated animals, Sham-MLT: sham-operated animals MLT (10 mg/kg), CCI-VEH: pain-induced animals, and CCI-MLT: pain-induced animals received MLT (10 mg/kg), mean ± standard deviation, n = 6 per group.
The percentage of TUNEL-positive cells was calculated in the PFC, CA1, CA3, and DG of the study groups to further examine the number of apoptotic neurons. The percentage of TUNEL-positive cells in the PFC, CA1, CA3, and DG of the CCI-VEH group increased compared with that of the Sham-VEH and Sham-MLT groups (Fig. 6A–E, P < 0.0001 and P < 0.0001, respectively). MLT reduced the percentage of TUNEL-positive cells in the PFC, CA1, CA3, and DG of the CCI-MLT group compared with that of the CCI-VEH group (Fig. 6A–E, P < 0.0001).
Effects of melatonin (MLT) on (A) the percentage of TUNEL-positive cells in the hippocampus and prefrontal cortex (PFC) of chronic constriction injury (CCI)-induced rats. Comparing the percentage of TUNEL + cells in the (B) PFC, (C) CA1, (D) CA3, and (E) DG among different groups. Arrows show TUNEL + cells with dark brown appearance (TUNEL staining, 100 ×). Percentage of TUNEL-positive cells ****P < 0.0001 compared with the Sham-VEH group; ####P < 0.0001 compared with the MLT group, and ++++P < 0.0001 compared with the CCI-VEH group. Sham-VEH: sham-operated animals, Sham-MLT: sham-operated animals MLT (10 mg/kg), CCI-VEH: pain-induced animals, and CCI-MLT: pain-induced animals received MLT (10 mg/kg), mean ± standard deviation, n = 6 per group.
Therefore, NP-induced apoptosis of the cells in the CNS could be ameliorated by MLT administration.
Discussion
In this study, the effects of MLT on pain-induced anxiety- and depressive-like behavior were examined. The CCI model of the sciatic nerve with four loose ligatures was established to mimic NP in a rat model. The results of HPT and ADT confirmed heat hyperalgesia and cold allodynia, respectively, on days 3, 7, 14, and 21 days after the CCI induction surgery. Meanwhile, the results obtained from EPMT, FST, TST, and OFT confirmed the anxiety- and depressive-like behaviors of the CCI-VEH group. In several studies, CCI has been introduced as the most common nerve damage in models used to evaluate NP26. NP is associated with increased pain sensitization (allodynia) and increased sensitivity to pain (hyperalgesia)27. Clinical results have indicated that chronic pain is a stress state that often induces mood disorders (e.g., anxiety and depression) and cognitive problems (e.g., concentration and memory deficits28,29. The CCI model of sciatic nerve injury has been used to examine mood disorders in several animal models30,31.
Our findings also confirmed that the percentage of dark neurons (dead neurons) and TUNEL-positive cells (apoptotic neurons) increased in the HC and PFC of CCI-induced rats. Besides, exposure to CCI injury significantly dysregulated the major anti-apoptotic (Bcl-2) and pro-apoptotic (Bax) genes in the HC and PFC. It was reported that NP and mood disorders share some common pathogenic mechanisms32. The sensory pathways of NP involve the same brain areas, including the insular cortex, PFC, HC, thalamus, anterior cingulate, and amygdala, that are injured during mood disorders33,34. Structural alterations of these regions verify the coexistence of pain and mood disorders35. The atrophy of HC has been associated with depressive symptoms in pain conditions36,37. In several studies, the volumes of the HC and PFC significantly decrease in patients with depression and closely correlate with depression severity32,38,39. In addition, the results of human and animal studies on depression have indicated the structural alterations, including reduction of spine density and atrophy and loss of neuronal cells in the HC and PFC40,41,42,43.
In postmortem investigations, a reduced number of synapses and deteriorated synaptic functions in the PFC have been demonstrated in individuals with depression44. Similarly, the apoptosis and dysregulated gene expression of Bax and Bcl2 have been observed in the cortex and HC of animal models with depression45,46,47. It was reported that pain-induced apoptosis could be associated with two main mechanisms, including the stress caused by pain and chronic excitation of neurons involved in pain control and transmission48. Several types of stress, including forced swim stress, cold stress, and immobilization stress, can induce cell death stress and increase the vulnerability of hippocampal neurons to injury, leading to dendritic atrophy and neuronal loss in this region, and impairing hippocampal performance49,50. Therefore, depression following NP may be related to plasticity impairments and cellular apoptosis.
The molecular mechanisms underlying pain-induced apoptosis and neuronal loss remain unknown. Our findings showed the stimulation of the NF-κB/NLRP3 axis in the HC and PFC of CCI-induced animals. Moreover, the concentration of cytokines (IL-1β and IL-18) enhanced in the HC and PFC following the CCI induction surgery, confirming that NLRP3 was activated in these regions. The important roles of inflammasome activation and cytokine production have been confirmed in anxiety and depression. Preclinical and clinical studies have demonstrated that the activation of NLRP3 inflammasome pathways is involved in depression pathophysiology51. The hippocampal levels of NF-κB (a transcription factor responsible for upregulation of NLRP3, pro-IL-1β, and pro-IL-18 and NLRP3), NLRP3 (a key component of the NLRP3 inflammasome), Casp-1, IL-18, and IL-1β increase in the rat model of chronic stress-induced depression51. Conversely, chronic pain leads to hippocampal dysfunction through TNF-α overproduction in adult male rats52. Similarly, NP is related to the increased levels of pro-inflammatory mediators (e.g., TNFα, IL-1β, and IL-6) in the HC, cingulum, and striatum of animals with CCI-induced nerve injury53. In another study, spared nerve injury (SNI)-induced depression was associated with the activation of microglia through the upregulation of Iba1 and CD11b and increased levels of pro-inflammatory mediators (IL-1β, TNF-α, and CD68) in the PFC54. Additionally, NP-induced depressive-like behaviors are correlated with the activation of hippocampal NLRP151 and NLRP355 inflammasomes. Moreover, the dysregulation of Bax/Bcl2 levels correlated with increased apoptosis is likely associated with the exacerbated dysfunction of NLRP3 inflammasomes and upregulation of inflammatory cytokines. Subsequently, chronic NP-induced pathological alterations result in anxiety- and depressive-like phenotypes. Therefore, inflammasomes likely form a connection between stressful conditions and activation of the innate immune system and subsequently induce pathophysiological alterations in the PFC and HC.
For efficient pain management, disorders coexisting with pain should be treated. In this study, MLT was used to alleviate pain-induced anxiolytic-like behaviors and depressive-like behaviors. In preclinical and clinical studies, MLT has been introduced as an effective agent in treating pain56,57 and emotional and cognitive disorders through several mechanisms58,59. MLT has been used as an analgesic agent to relieve pain in human and animal models60,61. Furthermore, exogenous MLT attenuates manifestations of anxiety and depression by restoring the disrupted levels of MLT rhythms62. It can also enhance the effects of fluoxetine in the treatment of depressive-like behaviors by regulating neurotrophins in the HC63.
Our behavioral studies confirmed that MLT (10 mg/kg) exerted analgesic, anti-anxiety, and antidepressant effects via anti-apoptotic and anti-inflammatory signaling pathways. The administration of MLT diminished the percentage of dark neurons and TUNEL-positive cells in the HC and PFC, indicating that MLT elicited neuroprotective effects. The neuroprotective and anti-apoptotic properties of MLT were mediated by regulating Bax and Bcl2 genes in the HC and PFC. However, the synthesis of NF-κB/NLRP3 axis components (NF-κB, NLRP3, ASC, and Casp-1) decreased in the HC and PFC of the rats treated with MLT. The modulation of NLRP3 inflammasome function resulted in the decreased synthesis of IL-1β and IL-18 in the HC and PFC of the treated rats. These results confirmed that MLT attenuated the CCI-induced depressive-like behaviors and anxiolytic-like behaviors and reduced cell death in the HC and PFC by regulating the Bax/Bcl2 and NF-κB/NLRP3 inflammasome axis. In addition, MLT has several mechanism pathways to exert its beneficial effects. The regulation of NLRP3 inflammasome mainly mediates its anti-inflammatory effect64. In several pathological conditions, MLT has improved the alterations via the regulation of NLRP3 inflammasome and reduction of pro-inflammatory cytokines (e.g., IL-1β)65,66,67,68. MLT has exerted anti-inflammatory effects by regulating NLRP3 inflammasome-related components in several neurological disorders. For instance, MLT was also demonstrated to protect against brain damage induced by subarachnoid hemorrhage (SAH) via the suppression of NLRP3 inflammasome-related components, including ASC, cleaved Casp-1, IL-1β, and IL-669,70. MLT also attenuated neuroinflammation in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced model of Parkinson’s disease through the suppression of microglial activation, downregulation of the NLRP3 inflammasome components, and inhibition of IL-1β secretion71. In line with our findings, MLT was reported to alleviate lipopolysaccharide (LPS)-induced depression through the modulation of microglial NLRP3 inflammasome72. Furthermore, the antidepressant effects of MLT were found to be related to the modulation of neuroinflammation by regulating hippocampal NF-κB phosphorylation and cytokines (TNFα, IL-1β, and IL-6) in the LPS-induced depression model73,74. MLT also causes anti-apoptotic effects by the modulation of Casp-3 activation75. In a transient cerebral ischemia model, MLT exerts neuroprotection by modulating Bax and Bcl2 activity and regulating DNA repair capacity and apoptosis76. MLT induces anti-inflammatory effects primarily by suppressing inflammasome activation75. Interestingly, MLT can diminish neuroinflammation, brain injury, and deficits by suppressing NLRP3 inflammasome and apoptosis70.
Materials and methods
Animals
In this study, 52 male adult Sprague–Dawley rats (180–200 g) were housed in a quiet standard environment at a constant temperature of 23 °C ± 2 °C and humidity of ~ 50% on a 12 h light/12 h dark cycle with food and water available ad libitum. This study was approved by the Ethics Committee of Animal Experiments at the University of Chinese Academy of Sciences and performed under the Guide for the Care and Use of Laboratory Animals (8th edition, Washington, DC: The National Academies Press). This study was done in accordance with ARRIVE guidelines.
Experimental design
The rats were divided into four main groups (n = 13 rats per group): (1) In the Sham-VEH group, rats operated without ligation were given a vehicle; (2) in the Sham-MLT group, rats operated without ligation were intraperitoneally (IP) injected with MLT (10 mg/kg); (3) in the CCI-VEH group, model animals were administered with the vehicle, and (4) in the CCI-MLT group, CCI-induced animals were IP injected with MLT (10 mg/kg)60,73. The vehicle and MLT were injected for 14 days post-surgery. MLT doses were selected based on a previous study77. Phosphate-buffered saline (PBS) + 0.1% dimethyl sulphoxide (Sigma-Aldrich) was used as the vehicle of MLT (Sigma-Aldrich). The study protocol is shown in Fig. 1A.
Surgery
The CCI model was established via loosely tied ligatures to induce NP-related depressive- and anxiety-like behaviors in animals. Briefly, the surgery was performed under anesthesia by interjecting a mixture of ketamine/xylazine (90/10 mg/kg). Firstly, the hair on the back of the left hind limb was shaved, and a 3-cm long mid-thigh incision was made. Then, we dissected the gluteus and biceps femoris muscles and used the common sciatic nerve for CCI induction. Secondly, four loose ligatures with silk sutures (4-0) were placed around the sciatic nerve. The skin and muscular layers were then sutured, and the antibiotic powder was topically applied to the wound to prevent infection. Meanwhile, the rats in the sham group were subjected to the same surgical procedure but without actual nerve ligation. After recovery, they were placed in cages and given free access to food and water.
Behavioral tests
An HPT and an ADT were performed to assess NP in different groups. The interval time between the two tests was 2 h. In addition, EPMT, FST, TST, and OFT were conducted to evaluate the anxiety-like and depressive-like behaviors of the animals78,79. All the tests were done by a blind observer under the schedule depicted in Fig. 1A.
Hot plate test (heat hyperalgesia stimulation)
The HPT was performed to evaluate the thermal nociceptive threshold on days 0 (before surgery), 3, 7, 14, and 21 of the investigation. The rats were placed one by one on the surface of a hot plate at 52 °C. A cutoff time of 20 s was set to prevent injury. The withdrawal latency in terms of the first response shaking, jumping, paw withdrawal, or paw licking, was recorded in seconds (35).
Acetone drop test (paw cold allodynia)
ADT was conducted to determine cold allodynia in rats on days 0 (before surgery), 3, 7, 14, and 21 of the investigation. Their hind paw was placed over a mesh wire, and the pain was induced by spraying acetone (100 μl) on the plantar surface of the rat’s paw. The reaction of the rats to acetone was recorded in 20 s. The following criteria described by Flatters and Bennett were used to score each rat: no reaction (0); quick withdrawal stamp or flick of the paw (1), prolonged flicking or repeated withdrawal (≥ 2) of the paw (2), and repeated flicking with the licking of the paw (3). This assay was performed three times with an interval of 5 min, and the minimum and maximum scores were 0 and 9, respectively80.
Elevated plus maze test
The EPMT was done on day 22 post-surgery. In the EPMT, an apparatus made of two open arms (50 cm × 10 cm) crossing at a right angle and two closed arms (50 cm × 10 cm × 40 cm) extending from a central platform (10 cm × 10 cm) was used. During the investigation, the rat was individually placed in the central part facing an open arm and allowed to explore freely in the open and closed arms for 5 min. The parameters, including close/open arm times, were recorded.
Forced swimming test
The FST was done on days 23 and 24 post-surgery. FST was done on day 2. In the FST, each rat was forced to swim in a glass cylinder (80 cm height and 30 cm diameter) filled with tap water to a height of 40 cm (23–25 °C water). Before the test, the rats were trained by placing them in the cylinder for 1 day. The procedure was recorded for 4 min as a test session, and the last 3 min were evaluated for behavioral alterations. The duration of immobility, swimming, and climbing was calculated during the test session.
Tail suspension test
The TST was done on day 25 post-surgery. In the TST, each rat was suspended by the tail 50 cm above the floor one by one using adhesive tape for 6 min; afterward, the duration of immobility was calculated for 4 min.
Open field test
The OFT was performed on day 26 post-surgery. In the OFT, we used an apparatus consisting of an open-field box (100 cm × 100 cm × 50 cm) and an arena surrounded by high walls. Its bottom was subdivided into 16 equal squares. The rats were individually placed in the central zone of the apparatus and freely allowed to explore for 5 min. The delay in the central and peripheral zones was calculated.
Tissue preparation
For histopathological studies, the rats were transcardially perfused under deep anesthesia and fixed with a fixative solution (containing 4% paraformaldehyde and formalin). Their brains (n = 6 per group) were obtained and postfixed in formalin for 24 h. For molecular investigations, whole fresh brains (n = 6) were removed under anesthesia and immediately put in ice-cold PBS (pH 7.4). The brain was bisected along the mid-sagittal plane, and the thalamic tissue of one hemisphere was peeled out to expose the ventricular surface of HC tissue. The entire HC was removed through a spatula tip along the length of the hippocampal fissure. In addition, the medial PFC (mPFC) was harvested from each hemisphere (coordinates, bregma: AP = 4.64–2.60 mm, ML = 2.40–5.40 mm, and DV = 1.20 mm)81. The fresh HC and PFC samples were collected and stored at − 80 °C.
Molecular evaluations
RNA extraction and quantitative polymerase chain reaction
A quantitative polymerase chain reaction (qPCR) was conducted to quantify the expression of related genes in the HC and PFC samples. Briefly, TriPure™ isolation reagent (Sigma-Aldrich) was used for total RNA extraction. The purity of the total RNA was quantified using a NanoDrop spectrophotometer. cDNA was synthesized using a PrimeScript one-step RT-PCR kit (Takara Biotechnology Co., Ltd.) at 42 °C for 1 h. qPCR was performed with an SYBR reagent, and reactions were run using a StepOnePlus real-time PCR machine. Beta2-microglobulin (B2M) was utilized as a housekeeping gene, and the fold change was computed as relative gene expression via the 2-ΔΔCT method82. The PCR primers are listed in Table 1.
Enzyme-linked immunosorbent assays
The levels of proteins (NF-κB, NLRP3, ASC, Casp-1, IL-1β, and IL-18) in the HC and PFC were assessed by enzyme-linked immunosorbent assay (ELISA) kits (Bio-Techne, Minneapolis, MN, USA) following the manufacturers’ instructions. The HC and PFC of each rat (n = 6 rats per group) were extracted immediately after the behavioral tests. These samples were homogenized in a tissue homogenizer 20 times with ice-cold lysis buffer. Moreover, the homogenate was centrifuged (2500 rpm/10 min) to separate the supernatant and utilized for ELISA. Optical density was then obtained.
Western blot
Fresh HC and PFC samples were collected (n = 6 in each group) to measure the protein levels of apoptotic factors. The tissues were homogenized in a lysis buffer and centrifuged at 15,000 rpm (10 min, at 4 °C). The total protein levels from each experiment were evaluated using a total protein kit (Micro, Sigma, USA). After protein denaturation, we exposed 5 μg of protein from each experiment to 10% SDS-PAGE and transferred it to a polyvinylidene difluoride (PVDF) transfer membrane (Sigma, USA). Then, the blots were blocked with blocking buffer (5% skimmed milk powder in PBS) at room temperature (20–22 °C) for 2 h. Additionally, they were incubated with antibodies against Bax (1/1000 v/v, Cat: ab216494, Abcam, Germany) and Bcl2 (1:1000, Cat: ab196495, Abcam, Germany) overnight at 4 °C and washed four times with 0.1% Tween 20 in PBS. The samples were subsequently incubated with HRP-conjugated secondary antibody (1/10,000 v/v) at room temperature for 1 h. Protein bands were visualized with a luminescent substrate solution (Sigma, USA) and quantified using ImageJ (NIH, USA). GAPDH (Cat: ab181602, Abcam, Germany) was used for normalization. Images of blots with adequate length and membrane edges could not be provided because the blots were cut prior to hybridisation with antibodies and scanned at inside of blots, respectively.
Histopathological investigations
The fixed tissues were processed using a tissue processor. Coronal sections (4–5 µm) were made with a microtome (Leica RM 2245, Wetzlar, Germany) after paraffin blocks were prepared. The paraffin sections were dewaxed, dehydrated, mounted on slides, stained with cresyl violet, and subjected to terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL).
Cresyl violet staining (Nissl staining)
Dark neurons (hyper-electron and hyper-basophil density) were visualized by Nissl staining83. The sections were stained with a cresyl violet solution in accordance with the Nissl staining protocol. Afterward, four sections were randomly chosen and evaluated under a light microscope (CX31, Tokyo, Japan) with 100× magnification. Microscopic images were prepared from the HC and PFC regions. The intact and dark neurons in CA1, CA3, DG, and PFC fields were counted in the × 100 images, and the percentage of dark neurons was calculated in each section.
TUNEL assay
The TUNEL assay (Roche, Germany) was performed to detect the apoptotic DNA fragmentation in neurons of the HC and PFC. In this assay, the prepared sections were washed with 10 mM Tris–HCl (pH 7.6), and endogenous peroxidase activity was suppressed by incubating with methanol + 0.3% H2O2 for 10 min. Next, the sections were incubated in proteinase K in PBS at 37 °C for 30 min. They were further incubated in a TUNEL reaction mixture of transferase enzyme and labeling solutions for 1 h and HRP-conjugated anti-fluorescein antibody at 37 °C for 30 min. Peroxidase was visualized with 1% 3,3ʹ-diaminobenzidine, and the sections were counterstained with hematoxylin.
Statistical analysis
Data were statistically analyzed using SPSS® (ver. 22) and GraphPad Prism (ver. 8.4.3). One-way analysis of variance and post hoc comparison test (Tukey’s) were conducted to find differences between study groups. Data were considered significantly different when P < 0.05.
Conclusions
In summary, our findings demonstrated that the exposure of animals to chronic NP strongly stimulated the NF-κB/NLRP3 axis. It not only increased the concentrations of cytokines, including IL-1β and IL-18, in the PFC and HC, but also caused anxiety- and depressive-like behaviors. Furthermore, the changes in the levels of the main regulators of apoptosis (Bax and Bcl2) and the enhanced percentage of apoptotic cells in the PFC and HC were associated with chronic NP. MLT might show analgesic and antidepressant properties by mitigating apoptosis and neuroinflammation. Furthermore, MLT might be effective against chronic NP-mediated anxiety- and depressive-like behaviors by regulating Bax/Bcl2 and NF-κB/NLRP3 signaling pathways in the PFC and HC. Our results suggested that MLT treatment might be a valuable strategy to attenuate apoptosis and neuroinflammation associated with NP-induced depressive-like behaviors and anxiolytic-like behaviors (Fig. 7).
The schematic summary of the study. Exposure of animals to chronic NP activated the NF-κB/NLRP3 axis in the HC and PFC of CCI-induced animals. Subsequently, the levels of cytokines (IL-1β and IL-18) enhanced in the HC and PFC in the CCI animals. Cytokines dysregulated the protein levels of bax and Bcl2 and resulted in apoptosis in the HC and PFC. MLT administration attenuated the above alterations.
Limitations
Our study had some limitations. There are sex differences in response to pain, and chronic pain affects a higher proportion of women worldwide than men. In the present study, we only considered male rats, and female animals should be tested in future studies. The present study explored the behaviors and neuro-inflammatory mechanisms, while other mechanisms, e.g., autophagy pathways, can be studied in the future.
Data availability
Data are available on request from TM.
References
Li, X.Y. et al. Maladaptive plasticity and neuropathic pain. Neural Plast. 2016, 4842159. https://doi.org/10.1155/2016/4842159 (2016).
Jensen, T. S. & Finnerup, N. B. Allodynia and hyperalgesia in neuropathic pain: Clinical manifestations and mechanisms. Lancet Neurol. 13, 924–935. https://doi.org/10.1016/S1474-4422(14)70102-4 (2014).
Armbrecht, E. et al. Economic and humanistic burden associated with noncommunicable diseases among adults with depression and anxiety in the United States. J. Med. Econ. 23, 1032–1042. https://doi.org/10.1080/13696998.2020.1776297 (2020).
Hooten, W. M. Chronic pain and mental health disorders: Shared neural mechanisms, epidemiology, and treatment. Mayo Clin. Proc. 91, 955–970. https://doi.org/10.1016/j.mayocp.2016.04.029 (2016).
Burke, N. N., Finn, D. P. & Roche, M. Neuroinflammatory mechanisms linking pain and depression. Mod Trends Pharmacopsychiatry 30, 36–50. https://doi.org/10.1159/000435931 (2015).
Bär, K.J. et al. Pain perception in major depression depends on pain modality. Pain 117, 97–103. https://doi.org/10.1016/j.pain.2005.05.016 (2005).
Yang, S. & Chang, M. C. Chronic pain: Structural and functional changes in brain structures and associated negative affective states. Int. J. Mol. Sci. 20, 3130. https://doi.org/10.3390/ijms20133130 (2019).
Ong, W.Y., Stohler, C. S. & Herr, D. R. Role of the prefrontal cortex in pain processing. Mol. Neurobiol. 56, 1137–1166. https://doi.org/10.1007/s12035-018-1130-9 (2019).
Mokhtari, T., Tu, Y. & Hu, L. Involvement of the hippocampus in chronic pain and depression. Brain Sci. Adv. 5, 288–298. https://doi.org/10.26599/BSA.2019.9050025 (2019).
Ren, K. & Dubner, R. Neuron-glia crosstalk gets serious: Role in pain hypersensitivity. Curr. Opin. Anaesthesiol. 21, 570. https://doi.org/10.1097/ACO.0b013e32830edbdf (2008).
Tiwari, V., Guan, Y. & Raja, S. N. Modulating the delicate glial-neuronal interactions in neuropathic pain: Promises and potential caveats. Neurosci. Biobehav. Rev. 45, 19–27. https://doi.org/10.1016/j.neubiorev.2014.05.002 (2014).
Campos, A. C. P., Antunes, G. F., Matsumoto, M., Pagano, R. L. & Martinez, R. C. R. Neuroinflammation, pain and depression: An overview of the main findings. Front. Psychol. 11, 1825. https://doi.org/10.3389/fpsyg.2020.01825 (2020).
Ji, R. R., Xu, Z. Z. & Gao, Y. J. Emerging targets in neuroinflammation-driven chronic pain. Nat. Rev. Drug Discov. 13, 533–548. https://doi.org/10.1038/nrd4334 (2014).
Jiang, B. C., Liu, T. & Gao, Y. J. Chemokines in chronic pain: Cellular and molecular mechanisms and therapeutic potential. Pharmacol. Ther. 212, 107581. https://doi.org/10.1016/j.pharmthera.2020.107581 (2020).
Adriana del Rey, A., Martina, M. & Besedovsky, H. O. Chronic neuropathic pain-like behavior and brain-borne IL-1β. Ann. N. Y. Acad. Sci. 1262, 101. https://doi.org/10.1111/j.1749-6632.2012.06621.x (2012).
Kim, H. et al. Brain indoleamine 2,3-dioxygenase contributes to the comorbidity of pain and depression. J. Clin. Investig. 122, 2940–2954. https://doi.org/10.1172/jci61884 (2012).
del Rey, A., Apkarian, A. V., Martina, M. & Besedovsky, H. O. Chronic neuropathic pain-like behavior and brain-borne IL-1β. Ann. N. Y. Acad. Sci. 1262, 101–107. https://doi.org/10.1172/JCI61884 (2012).
Liu, T. et al. Resveratrol ameliorates estrogen deficiency-induced depression-and anxiety-like behaviors and hippocampal inflammation in mice. Psychopharmacology 236, 1385–1399. https://doi.org/10.1007/s00213-018-5148-5 (2019).
Tabeefar, H., Chang, F., Cooke, M. & Patel, T. Community pharmacists and chronic pain: A qualitative study of experience, perception, and challenges. Can. J. Pain 4, 29–39. https://doi.org/10.1080/24740527.2020.1749516 (2020).
Pourmand, A. et al. Virtual reality as a clinical tool for pain management. Curr. Pain Headache Rep. 22, 1–6. https://doi.org/10.1007/s11916-018-0708-2 (2018).
Azizi, M. et al. Effects of exogenous melatonin on MAM induced lung injury and lung development in mice offspring. Tanaffos 19, 66 (2020).
Alghamdi, B. The neuroprotective role of melatonin in neurological disorders. J. Neurosci. Res. 96, 1136–1149. https://doi.org/10.1002/jnr.24220 (2018).
Laste, G. et al. Melatonin administration reduces inflammatory pain in rats. J. Pain Res. 5, 359. https://doi.org/10.2147/JPR.S34019 (2012).
Naseri, S. et al. Radio-protective effects of melatonin on subventricular zone in irradiated rat: Decrease in apoptosis and upregulation of nestin. J. Mol. Neurosci. 63, 198–205. https://doi.org/10.1007/s12031-017-0970-5 (2017).
Azizi, M. et al. Therapeutic effect of perinatal exogenous melatonin on behavioral and histopathological changes and antioxidative enzymes in neonate mouse model of cortical malformation. Int. J. Dev. Neurosci. 68, 1–9. https://doi.org/10.1016/j.ijdevneu.2018.03.008 (2018).
Kukkar, A., Singh, N. & Jaggi, A. S. Neuropathic pain-attenuating potential of aliskiren in chronic constriction injury model in rats. J. Renin Angiotensin Aldosterone Syst. 14, 116–123. https://doi.org/10.1177/1470320312460899 (2013).
Yam, M. F. et al. General pathways of pain sensation and the major neurotransmitters involved in pain regulation. Int. J. Mol. Sci. 19, 2164. https://doi.org/10.3390/ijms19082164 (2018).
Fonseca-Rodrigues, D., Amorim, D., Almeida, A. & Pinto-Ribeiro, F. Emotional and cognitive impairments in the peripheral nerve chronic constriction injury model (CCI) of neuropathic pain: A systematic review. Behav. Brain Res. 399, 113008. https://doi.org/10.1016/j.bbr.2020.1130088 (2021).
Grilli, M. Chronic pain and adult hippocampal neurogenesis: Translational implications from preclinical studies. J. Pain Res. 10, 228. https://doi.org/10.2147/JPR.S146399 (2017).
Li, Q. et al. Effects of chronic electroacupuncture on depression- and anxiety-like behaviors in rats with chronic neuropathic pain. Evid. Based Complement Altern. Med. 2014, 158987. https://doi.org/10.1155/2014/158987 (2014).
McIlwrath, S. L. et al. Manganese-enhanced MRI reveals changes within brain anxiety and aversion circuitry in rats with chronic neuropathic pain-and anxiety-like behaviors. Neuroimage 223, 117343. https://doi.org/10.1016/j.neuroimage.2020.117343 (2020).
MacQueen, G. M., Yucel, K., Taylor, V. H., Macdonald, K. & Joffe, R. Posterior hippocampal volumes are associated with remission rates in patients with major depressive disorder. Biol. Psychiatry 64, 880–883. https://doi.org/10.1016/j.biopsych.2008.06.027 (2008).
Meerwijk, E. L., Ford, J. M. & Weiss, S. J. Brain regions associated with psychological pain: Implications for a neural network and its relationship to physical pain. Brain Imaging Behav. 7, 1–14. https://doi.org/10.1007/s11682-012-9179-y (2013).
Neugebauer, V., Galhardo, V., Maione, S. & Mackey, S. C. Forebrain pain mechanisms. Brain Res. Rev. 60, 226–242. https://doi.org/10.1016/j.brainresrev.2008.12.014 (2009).
Sheng, J., Liu, S., Wang, Y., Cui, R. & Zhang, X. The link between depression and chronic pain: Neural mechanisms in the brain. Neural Plast. https://doi.org/10.1155/2017/9724371 (2017).
Liu, M. G. & Chen, J. Roles of the hippocampal formation in pain information processing. Neurosci. Bull. 25, 237–266. https://doi.org/10.1007/s12264-009-0905-4 (2009).
Zhu, C., Xu, Q., Wang, C., Mao, Z. & Lin, N. Evidence that CA3 is underling the comorbidity between pain and depression and the co-curation by Wu-Tou decoction in neuropathic pain. Neuropathic Pain 7, 1–14. https://doi.org/10.1038/s41598-017-12184-y (2017).
Chan, S. W. et al. Hippocampal volume in vulnerability and resilience to depression. J. Affect. Disord. 189, 199–202. https://doi.org/10.1016/j.jad.2015.09.021 (2016).
Gold, P. W., Machado-Vieira, R. & Pavlatou, M. G. Clinical and biochemical manifestations of depression: Relation to the neurobiology of stress. Neural Plast. 2015, 581976. https://doi.org/10.1155/2015/581976 (2015).
Ruilian, L. et al. H2S-mediated aerobic exercise antagonizes the hippocampal inflammatory response in CUMS-depressed mice. J. Affect. Disord. 283, 410–419. https://doi.org/10.1016/j.jad.2021.02.005 (2021).
Zhang, N. et al. Increased ASL-CBF in the right amygdala predicts the first onset of depression in healthy young first-degree relatives of patients with major depression. J. Cereb. Blood Flow Metab. 40, 54–66. https://doi.org/10.1177/0271678X19861909 (2020).
Sanacora, G. & Banasr, M. From pathophysiology to novel antidepressant drugs: Glial contributions to the pathology and treatment of mood disorders. Biol Psychiatry. 73, 1172–1179. https://doi.org/10.1016/j.biopsych.2013.03.032 (2013).
Lucassen, P. J. et al. Stress, depression and hippocampal apoptosis. CNS Neurol. Disord. Drug Targets 5, 531–546. https://doi.org/10.2174/187152706778559273 (2006).
Kang, H. J. et al. Decreased expression of synapse-related genes and loss of synapses in major depressive disorder. Nat. Med. 18, 1413–1417. https://doi.org/10.1038/nm.2886 (2012).
Kosten, T. A., Galloway, M. P., Duman, R. S., Russell, D. S. & D’sa, C. Repeated unpredictable stress and antidepressants differentially regulate expression of the bcl-2 family of apoptotic genes in rat cortical, hippocampal, and limbic brain structures. Neuropsychopharmacology 33, 1545–1558. https://doi.org/10.1038/sj.npp.1301527 (2008).
Wang, Y., Xiao, Z., Liu, X. & Berk, M. Venlafaxine modulates depression-induced behaviour and the expression of Bax mRNA and Bcl-xl mRNA in both hippocampus and myocardium. Hum. Psychopharmacol. 26, 95–101. https://doi.org/10.1002/hup.1177 (2011).
Wang, X. et al. Resveratrol reverses chronic restraint stress-induced depression-like behaviour: Involvement of BDNF level, ERK phosphorylation and expression of Bcl-2 and Bax in rats. Brain Res. Bull. 125, 134–143. https://doi.org/10.1016/j.brainresbull.2016.06.014 (2016).
Jalalvand, E., Javan, M., Haeri-Rohani, A. & Ahmadiani, A. Stress- and non-stress-mediated mechanisms are involved in pain-induced apoptosis in hippocampus and dorsal lumbar spinal cord in rats. Neuroscience 157, 446–452. https://doi.org/10.1016/j.neuroscience.2008.08.052 (2008).
McEwen, B. S. The neurobiology of stress: From serendipity to clinical relevance. Brain Res. 886, 172–189. https://doi.org/10.1016/S0006-8993(00)02950-4 (2000).
Watanabe, Y., Gould, E. & McEwen, B. S. Stress induces atrophy of apical dendrites of hippocampal CA3 pyramidal neurons. Brain Res. 588, 341–345. https://doi.org/10.1016/0006-8993(92)91597-8 (1992).
Kaufmann, F. N. et al. NLRP3 inflammasome-driven pathways in depression: Clinical and preclinical findings. Brain Behav. Immun. 64, 367–383. https://doi.org/10.1016/j.bbi.2017.03.002 (2017).
Ren, W. J. et al. Peripheral nerve injury leads to working memory deficits and dysfunction of the hippocampus by upregulation of TNF-α in rodents. Neuropsychopharmacology 36, 979–992. https://doi.org/10.1038/npp.2010.236 (2011).
Al-Amin, H., Sarkis, R., Atweh, S., Jabbur, S. & Saadé, N. Chronic dizocilpine or apomorphine and development of neuropathy in two animal models II: Effects on brain cytokines and neurotrophins. Exp. Neurol. 228, 30–40. https://doi.org/10.1016/j.expneurol.2010.11.005 (2011).
Xu, N. et al. Spared nerve injury increases the expression of microglia M1 markers in the prefrontal cortex of rats and provokes depression-like behaviors. Front. Neurosci. 11, 209. https://doi.org/10.3389/fnins.2017.00209 (2017).
Liu, P., Chen, J., Ma, S., Zhang, J. & Zhou, J. Albiflorin attenuates mood disorders under neuropathic pain state by suppressing the hippocampal NLRP3 inflammasome activation during chronic constriction injury. Int. J. Neuropsychopharmacol. 24, 64–76. https://doi.org/10.1093/ijnp/pyaa076 (2021).
Li, Q. et al. Hippocampal PKR/NLRP1 inflammasome pathway is required for the depression-like behaviors in rats with neuropathic pain. Neuroscience 412, 16–28. https://doi.org/10.1016/j.neuroscience.2019.05.025 (2019).
Chen, W. W., Zhang, X. & Huang, W. J. Pain control by melatonin: Physiological and pharmacological effects. Exp. Ther. Med. 12, 1963–1968. https://doi.org/10.3892/etm.2016.3565 (2016).
Tonon, A. C., Pilz, L. K., Markus, R. P., Hidalgo, M. P. & Elisabetsky, E. Melatonin and depression: A translational perspective from animal models to clinical studies. Front. Psychiatry https://doi.org/10.3389/fpsyt.2021.638981 (2021).
Hansen, M. V. et al. The effect of MELatOnin on Depression, anxietY, cognitive function and sleep disturbances in patients with breast cancer. The MELODY trial: Protocol for a randomised, placebo-controlled, double-blinded trial. BMJ Open 2, e000647. https://doi.org/10.1136/bmjopen-2011-000647 (2012).
Zangiabadi, N. et al. Effects of melatonin in prevention of neuropathy in STZ-induced diabetic rats. Am. J. Pharmacol. Toxicol. 6, 59–67. https://doi.org/10.3844/ajptsp.2011.59.67 (2011).
Wilhelmsen, M., Amirian, I., Reiter, R. J., Rosenberg, J. & Gögenur, I. Analgesic effects of melatonin: A review of current evidence from experimental and clinical studies. J. Pineal Res. 51, 270–277. https://doi.org/10.1111/j.1600-079X.2011.00895.x (2011).
Tchekalarova, J., Stoynova, T., Ilieva, K., Mitreva, R. & Atanasova, M. Agomelatine treatment corrects symptoms of depression and anxiety by restoring the disrupted melatonin circadian rhythms of rats exposed to chronic constant light. Pharmacol. Biochem. Behav. 171, 1–9. https://doi.org/10.1016/j.pbb.2018.05.016 (2018).
Li, K. et al. Melatonin augments the effects of fluoxetine on depression-like behavior and hippocampal BDNF–TrkB signaling. Neurosci. Bull. 34, 303–311. https://doi.org/10.1007/s12264-017-0189-z (2018).
Esposito, E. & Cuzzocrea, S. Antiinflammatory activity of melatonin in central nervous system. Curr. Neuropharmacol. 8, 228–242. https://doi.org/10.2174/157015910792246155 (2010).
Zhang, Y. et al. Melatonin alleviates acute lung injury through inhibiting the NLRP3 inflammasome. J. Pineal Res. 60, 405–414. https://doi.org/10.1111/jpi.12322 (2016).
Arioz, B. I., Tarakcioglu, E., Olcum, M. & Genc, S. J. A. The role of melatonin on NLRP3 inflammasome activation in diseases. Antioxidants (Basel) 10, 1020. https://doi.org/10.3390/antiox10071020 (2021).
Ashrafizadeh, M. et al. Anti-inflammatory activity of melatonin: A focus on the role of NLRP3 inflammasome. Inflammation 44, 1207–1222. https://doi.org/10.1007/s10753-021-01428-9 (2021).
Rahim, I. et al. Melatonin administration to wild-type mice and nontreated NLRP 3 mutant mice share similar inhibition of the inflammatory response during sepsis. J. Pineal Res. 63, e12410. https://doi.org/10.1111/jpi.12410 (2017).
Cao, S. et al. Melatonin-mediated mitophagy protects against early brain injury after subarachnoid hemorrhage through inhibition of NLRP3 inflammasome activation. Sci. Rep. 7, 1–11. https://doi.org/10.1038/s41598-017-02679-z (2017).
Dong, Y. et al. Melatonin attenuated early brain injury induced by subarachnoid hemorrhage via regulating NLRP 3 inflammasome and apoptosis signaling. J. Pineal Res. 60, 253–262. https://doi.org/10.1111/jpi.12300 (2016).
Zheng, R. et al. Melatonin attenuates neuroinflammation by down-regulating NLRP3 inflammasome via a SIRT1-dependent pathway in MPTP-induced models of Parkinson’s disease. J. Inflamm. Res. 14, 3063–3075. https://doi.org/10.2147/JIR.S317672 (2021).
Arioz, B. I. et al. Melatonin attenuates LPS-induced acute depressive-like behaviors and microglial NLRP3 inflammasome activation through the SIRT1/Nrf2 pathway. Front. Immunol. 10, 1511. https://doi.org/10.3389/fimmu.2019.01511 (2019).
Ali, T. et al. Melatonin act as an antidepressant via attenuation of neuroinflammation by targeting Sirt1/Nrf2/HO-1 signaling. Front. Mol. Neurosci. 13, 96. https://doi.org/10.3389/fnmol.2020.00096 (2020).
Ali, T. et al. Melatonin prevents neuroinflammation and relieves depression by attenuating autophagy impairment through FOXO3a regulation. J. Pineal Res. 69, e12667. https://doi.org/10.1111/jpi.12667 (2020).
Tarocco, A. et al. Melatonin as a master regulator of cell death and inflammation: Molecular mechanisms and clinical implications for newborn care. Cell Death Dis. 10, 1–12. https://doi.org/10.1038/s41419-019-1556-7 (2019).
Sun, F. Y. et al. Neuroprotection by melatonin against ischemic neuronal injury associated with modulation of DNA damage and repair in the rat following a transient cerebral ischemia. J. Pineal Res. 33, 48–56. https://doi.org/10.1034/j.1600-079X.2002.01891.x (2002).
Stefanovic, B., Spasojevic, N., Jovanovic, P. & Dronjak, S. Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression. Can. J. Physiol. Pharmacol. 97, 685–690. https://doi.org/10.1139/cjpp-2018-0612 (2019).
Zhu, H. et al. Long-term stability and characteristics of behavioral, biochemical, and molecular markers of three different rodent models for depression. Brain Behav. 10, e01508. https://doi.org/10.1002/brb3.1508 (2020).
Belovicova, K., Bogi, E., Csatlosova, K. & Dubovicky, M. Animal tests for anxiety-like and depression-like behavior in rats. Interdiscip. Toxicol. 10, 40. https://doi.org/10.1515/intox-2017-0006 (2017).
Flatters, S. J. & Bennett, G. J. Ethosuximide reverses paclitaxel-and vincristine-induced painful peripheral neuropathy. Pain 109, 150–161. https://doi.org/10.1016/j.pain.2004.01.029 (2004).
Zhao, D. Q., Gong, S. N., Ma, Y. J. & Zhu, J. P. Medial prefrontal cortex exacerbates gastric dysfunction of rats upon restraint water-immersion stress. Mol. Med. Rep. 20, 2303–2315. https://doi.org/10.3892/mmr.2019.10462 (2019).
Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25, 402–408. https://doi.org/10.1006/meth.2001.1262 (2001).
Mahakizadeh, S. et al. Effects of chronic hypoxia on the expression of seladin-1/Tuj1 and the number of dark neurons of hippocampus. J. Chem. Neuroanat. 104, 101744. https://doi.org/10.1016/j.jchemneu.2020.101744 (2020).
Acknowledgements
This study was supported by the National Natural Science Foundation of China (No. 32000749 and 32071061) and the Scientific Foundation of Institute of Psychology, Chinese Academy of Science (No. E0CX491005). TM would like to acknowledge financial support from CAS-TWAS president's fellowship program 2019.
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L.H. and T.M. contributed substantially to the conception and design of the study. T.M. contributed to performing the experiments and analyzing the data. L.H., T.M., and L.P.Y. drafted or provided critical revisions to the article.
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Mokhtari, T., Yue, LP. & Hu, L. Exogenous melatonin alleviates neuropathic pain-induced affective disorders by suppressing NF-κB/ NLRP3 pathway and apoptosis. Sci Rep 13, 2111 (2023). https://doi.org/10.1038/s41598-023-28418-1
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DOI: https://doi.org/10.1038/s41598-023-28418-1
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