Abstract
Biofilms of the gastroenteric pathogen C. jejuni may serve an important role in the transmission of infection from reservoirs of infection to humans. Herein, we undertook a combinatorial approach examining differential gene expression and protein abundance during biofilm formation in C. jejuni. Biofilms induced a substantial rearrangement of the C. jejuni transcriptome and proteome, with ~600 genes differentially expressed when compared to planktonic cells. Genes and proteins induced in biofilms were involved in iron metabolism and acquisition, cell division, glycan production and attachment, while those repressed were associated with metabolism, amino acid usage, and large tracts of the chemotaxis pathway. We further examined the role of chemotaxis in C. jejuni biofilm formation by examining isogenic strains with deletions of the cheV and cheW signal transduction genes. Both ∆cheV and ∆cheW exhibited a significant decrease in directed motility when compared to wild-type C. jejuni as well as demonstrating an increase in autoagglutination ability and biofilm formation. A subtle difference was also observed between the phenotypes of ∆cheV and ∆cheW mutants, both in motility and biofilm formation. This suggests roles for CheV and CheW and may present signal transduction as a potential method for modulating C. jejuni biofilm formation.
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Introduction
Campylobacter jejuni is a Gram-negative bacterium responsible for the majority of bacterial gastroenteritis cases in developed nations. C. jejuni is a common commensal organism of avians and is transmitted to humans through contaminated food products or water. Once ingested, C. jejuni migrates through the gastrointestinal tract, colonising, and infecting cells of the intestine1. C. jejuni infections have also been implicated in the development of post infection complications such as the autoimmune neuro-degenerative conditions, Guillain-Barré and Miller-Fisher syndromes2.
C. jejuni biofilms have been suggested to play a role in the transmission of infection between chickens and human hosts3. Exacting and microaerophilic, C. jejuni may utilize biofilms as a method of surviving nutrient deficient and oxygenated environments, such as food preparation and storage4, in order to remain viable. To date, a number of factors have been shown to play a role in C. jejuni biofilm formation. A large number of protein families have been implicated, including glycosylation pathways5, DPS (DNA-binding protein from starved cells) proteins6, and post-transcriptional regulators7. An interesting aspect of C. jejuni biofilm formation is the impact of motility and chemotaxis gene expression8,9.
Chemotaxis in C. jejuni is a two-component signal transduction system where a change in conformation of the chemoreceptors, known as transducer-like proteins (Tlps), directs autophosphorylation of a histidine kinase, CheA. CheA phosphorylates CheY, which acts as a cytoplasmic Response Regulator (RR) that migrates to the flagella motor where it can influence changes in flagellar rotation. The phosphorylation of CheAY also serves to activate CheB, a methyltransferase that demethylates the signalling domain of Tlps upon the binding of chemorepellents. C. jejuni possesses an additional scaffolding protein, CheV. CheV is comprised of a CheW-like domain and an additional CheY-like RR domain at the C-terminus. The CheW-like domain of CheV has been shown to play a similar role to CheW and the two share conserved sequence10,11. Currently, the function of the RR domain of CheV is unknown, although there is evidence it may be involved in sensory adaptation probably because CheB of C. jejuni lacks an RR domain present in CheB proteins of other bacteria12. A number of C. jejuni chemoreceptors have been shown to have a higher affinity for either CheV or CheW in C. jejuni. The Tlp2, Tlp3 (CcmL), Tlp4 paralogues and Tlp11 (CcrG) chemoreceptor signalling domains have been shown to interact non-preferentially with either CheV or CheW proteins13,14,15, whereas the Tlp1 chemoreceptor has demonstrated strong interactions with only the CheV homologue16.
Elements of this chemotaxis pathway have been shown to play a significant role in the formation of biofilm in C. jejuni. General motility is required, as deletion of components of the flagella complex results in a loss of biofilm forming ability17. Interestingly, chemoreceptors appear to influence both increases and decreases in biofilm formation. Deletions in Tlp8, for example, result in decreased biofilm production, whereas deletions in Tlp3 increase biofilm levels. This may be due to the effect of chemoreceptors on motility, as the Tlp8 mutant demonstrates increased motility whilst the directed motility of a Tlp3 mutant is diminished due to its “tumble” phenotype bias8,14.
In this study, we utilize a comparative omics approach to reveal genes and proteins that are associated with C. jejuni biofilm growth. Analysis of the transcriptome and proteome demonstrated distinct variations between planktonic and biofilm states and revealed key roles for a number of families of proteins including those involved in chemotaxis. We further investigated the relationship between the chemotaxis pathway and C. jejuni biofilms, by specifically examining the role of the chemotaxis proteins CheV and CheW in C. jejuni biofilm formation.
Results
RNA transcription during planktonic and biofilm growth states
Detailed methods and accession numbers for RNA-seq datasets can be found in the corresponding Microbial Resource Announcement18.
RNA-seq analysis identified 1571 genes equating to ~96.8% coverage of the C. jejuni NCTC 11168 genome (Supplementary Table S1). 789 genes were considered as differentially expressed (DE) using a fold change cut-off of Log2 < −1 and > +1 (equivalent to a +/−2-fold change), with 427 up-regulated and 362 down-regulated transcripts in biofilm conditions (Supplementary Table S2). Increasing the stringency by including only those DE genes that met the false discovery rate (FDR) threshold of <0.01 removed 169 genes from further analysis. Therefore, a final total of 620 genes were considered DE at the +/−2-fold level of regulation, with 318 up- and 302 down-regulated genes (Fig. 1, Supplementary Table S2).
Volcano plot of C. jejuni NCTC 11168 gene expression during biofilm growth. Log2 (Fold Change) significance cut-off was +/− 1, with threshold for significance set at -log10 (FDR) < 2. A total of 1571 genes were identified (96.7% coverage) and 620 were significantly differentially expressed (blue shaded data points).
These DE genes were next subjected to functional cluster analysis using STRINGdb. DE genes that were up-regulated in C. jejuni NCTC 11168 biofilm growth (Supplementary Fig. S1a) were strongly associated with several functionally related clusters; including iron limitation and iron acquisition (enterochelin uptake, ceuBCD; and haemin uptake, chuABCD; cfbpBC, cfrA and several unique genes e.g. cj1384c and cj0730), ribosomal proteins and translation, amino acid biosynthesis and metabolism also related to signal molecule production and biofilm formation (metABEF; trpABD, hisABF1), cell division and peptidoglycan (pbpAC, murG, mreB) and production and attachment of N-glycans (e.g. pglABCDIG). Several smaller clusters were broadly related to transport of nutrients and small molecules (e.g. molybdenum, modABC; phosphate, pstABC), branched chain amino acid transport (livFGHKM) and co-location in operons with no currently assigned function (e.g. cj0727-cj0732; cj1658-cj1663; cj1581c-cj1583c). We also examined a subset of these DE genes that exhibited the greatest magnitude of change (>+5-fold; Supplementary Fig. S1b). This emphasised the changes associated with iron acquisition and iron-regulated genes; as well as molybdenum and phosphate acquisition, and clusters of genes with no known function (e.g. cj1660-cj1662; cj1581c-cj1583c).
Down-regulated DE genes (Supplementary Fig. S1) clustered into fewer functional groups, however major clusters were associated with chemotaxis and transducer-like proteins (cheBRWY and cj0019c, cj0144, cj1506c, cj1564; as well as cj0448c [homologous to biofilm dispersion protein bdlA]), amino acid uptake, utilization (sdaC, dcuA, aspAB) and metabolism (sdhABC), nitrate reductase (napABDGHL) and motility (flgABCHM, fliEL, flaG). Smaller clusters were again related to specific operons (e.g. cj1430c-cj1436c, cj0770c-cj0772c; cj0920c-cj0922c [encoding peb1A and peb1C] and cj0552-cj0554). Similar to the analysis performed for up-regulated genes, we next specifically looked at genes with large magnitude down-regulated fold changes (<−5-fold; Supplementary Fig. S1d), which further highlighted changes associated with motility, nutrient uptake and metabolism, and related genes of no known function.
LC-MS/MS proteomics ofC. jejuniNCTC 11168 biofilm growth
Label-based proteomics analysis by trypsin digest and LC-MS/MS was performed on biological triplicates and 1278 proteins (~78.7% of the 1623 C. jejuni NCTC 11168 genes in the Uniprot database) were identified in at least 1 biological replicate (Supplementary Table S3) by a minimum of 2 or more unique peptides. 11 proteins were identified where the corresponding transcripts were not detected by RNA-seq (Cj0185c, Cj0202c, Cj0455c, Cj0483, Cj0679, Cj0874c, Cj0876c, Cj0951c-Cj0952c, Cj1392-Cj1393). Log2 fold changes were converted to n-fold change for further analysis. Similar to the data observed by transcriptomics, biofilm growth resulted in a major remodelling of the C. jejuni proteome and 431 proteins were considered differentially abundant (DA) at a fold-change cutoff of +/−1.5-fold (Supplementary Table S4) with 220 present at increased abundance and 211 present at reduced abundance in C. jejuni biofilm growth. As for the DE gene expression analysis, we next subjected the DA proteins to functional cluster analysis using STRINGdb. The DA proteins present at increased biofilm abundance displayed major clusters associated with ribosomal proteins (translation), protein N-glycosylation and intracellular metabolic processes, including methionine metabolism (MetABEF; Supplementary Fig. S2). Another cluster was associated with antimicrobial resistance (predominantly CmeABC). DA proteins present at reduced levels in biofilm growth clustered into several major groups (Supplementary Fig. S2b), including ribosomal proteins, motility and chemotaxis proteins (FlgD, FliN, CheRY), proteins involved in respiration that allow the use of alternative electron acceptors under low oxygen conditions (e.g. nitrate reductase NapABDGL, formate and fumarate dehydrogenases [FdhABC; SdhABC] and Cj0264c-Cj0265c), nutrient acquisition and utilization (AspAB, AnsA, DcuA), a group of proteins involved in glycan biosynthesis (Cj1427c-Cj1438c), and a small cluster of functionally unknown proteins (Cj0411-Cj0413).
We next integrated the omics data to identify genes and their products that were closely associated with this model of biofilm growth in C. jejuni. The RNA-seq and proteomics data were aligned by fold-change and statistical analysis (Fig. 2a). The data showed that transcript and proteomics data correlated well (Spearman coefficient 0.767 with p < 0.001), although fold changes were generally considerably higher for the transcriptomics data (−52.33- to +25.45-fold) compared with the proteomics data (−9.38- to +14.42-fold). We also observed that larger RNA-seq-derived fold changes, particularly those that were up-regulated DE genes, mainly correlated with proteins that could not be detected by LC-MS/MS (of 48 genes up-regulated>8-fold only 11 could be identified at the protein level), most likely indicating that these transcripts (and proteins) are of lower starting abundances. Examination of the entire aligned dataset (transcripts and proteins both identified) using heat mapping software also showed that the two datasets typically correlated (as measured by direction of any expression/abundance change), although some specific outliers could be observed (Fig. 2b).
Comparisons of transcriptomics and proteomics. (A) Correlation plot of gene expression (x-axis) versus proteomics data y-axis). Spearman correlation coefficient = 0.767; (B) Heat map of gene expression (left column) and proteomics (left to right; mean; biological triplicate data) data, ranked according to fold change as determined by RNA-seq gene expression.
We considered such ‘outliers’ to be defined as a gene/protein with an opposite and significant DE/DA ratio (e.g. a significantly down-regulated gene showing a significantly up-regulated protein abundance). Only 11 genes/proteins were outliers; 7 with elevated gene transcript and significantly reduced protein abundance (rpmG, fdhU, cj0299, cj0908, cj1169c, ybeY and cj0648), and 4 with reduced transcript level and increased protein abundance (dps, ciaI, ftn and cj1053c) (Supplementary Table S5).
Biofilm formation
Since our integrated omics analysis strongly indicated repression of genes/proteins involved in chemosensing and chemotaxis (Figs. 3A and S3), we next examined the role of these functions in C. jejuni biofilms. Quantitative comparison of biofilm formed by wild-type C. jejuni NCTC 11168 O and ΔcheV and ΔcheW chemotaxis-deficient mutants, demonstrated that the cheV-deficient isogenic mutant strain had an approximately five-fold increase in biofilm formation, whilst the cheW-deficient mutant showed an ~three-fold increase in the ability to form biofilms (Fig. 3B–E).
Association of chemotaxis and chemosensory genes/proteins with biofilm formation. (A) Heat map showing gene expression and relative protein abundance of chemosensory and chemotaxis-associated genes during biofilm growth; (B) Dissolution of stained biofilm showing increased levels of biofilm formation, measured by absorbance at 600 nm, in CheV and CheW deficient mutants, (C–E) images of stained biofilm in 24-well plates show this same increase qualitatively.
Motility and autoagglutination of che mutants
Soft agar chemotaxis assays were conducted in order to compare the ability of wild-type, ΔcheV and ΔcheW C. jejuni to migrate through a solid support. Wild-type C. jejuni NCTC 11168 O showed an average migration of 28 mm from the inoculation site (Fig. 4A,B). Both ΔcheV (4.5 mm migration) and ΔcheW (6 mm migration) mutants showed significantly diminished migration when compared to the wild-type strain (p value of 0.033; Fig. 4A,C and D).
Analysis of motility and autoagglutination activity. (A) Migration (mm) of bacteria from the inoculation site for WT, ΔcheV and ΔcheW isogenic mutants in triplicate; (B–D) Images of the formed halos demonstrating decreased swarming motility by WT, ΔcheV and ΔcheW mutants; (E) Spectrophotomer readings of the upper phase of cell suspension following liquid culture demonstrate a lower absorbance in ΔcheV and ΔcheW strains when compared to WT, indicating an increased tendency for cells to autoagglutinate, (F–H) which was also evident when culture tubes were inspected.
Autoagglutination assays were carried out to determine the impact of diminished motility on intercellular adherence. Cells with a higher tendency to adhere to neighbouring cells cause increased settling and a lower absorbance of the upper phase. Both ΔcheV and ΔcheW mutants (Fig. 4G,H) showed a marked increase in clustering when compared to the wild-type control (Fig. 4F). This increase in autoagglutination was confirmed by assessing the absorbance of the upper phase of media in the culture tubes. A marked decrease was noted for both mutants (agglutination assays performed in triplicate), suggesting a large increase in autoagglutination (Fig. 4E).
Time-lapse microscopy
Time-lapse microscopy was conducted to highlight differences in overall biofilm formation in C. jejuni isogenic strains (Supplementary Fig. S4). Both ΔcheV and ΔcheW isogenic mutants exhibited a higher tendency to aggregate into cell clusters than wild-type C. jejuni. This is particularly evident during the interim stages of biofilm development such as seen at 15–20 hours. Wild-type C. jejuni showed a progressive spread of biofilm formation into a confluent monolayer, whereas ΔcheV and ΔcheW mutants appeared to congregate more readily into large microcolonies. In addition to the increased aggregates, a much larger biomass was evident in the ΔcheV and ΔcheW mutants when compared to the wild-type.
Discussion
Combining the use of RNA sequencing and proteomics enabled a global understanding of C. jejuni biofilm growth and demonstrated that significant remodelling of the transcriptome and proteome occurs in this lifestyle compared with planktonic culture at 42 °C. We identified many genes encoding protein families that were differentially expressed in biofilms including those involved with glycosylation, metabolism and chemotaxis, as well as many currently functionally poorly characterised gene clusters.
Generally, chemotaxis has long been established to play an important role in biofilm formation in C. jejuni, although there have been few studies that examine the role of signal transduction in biofilm formation. It has been suggested that the impaired motility of aflagellated mutants impacts the access of bacterial cells to an abiotic surface18,19,20. Mutations to cheW and cheV proteins also serve to diminish chemotaxis through soft agar in C. jejuni, as demonstrated by the decreased migration in Fig. 4. However, despite the decrease in migration, both ΔcheW and ΔcheV exhibited stark increases in the amount of biofilm formed, consistent with the significant biofilm-associated down-regulation of chemotaxis and chemosensory genes/proteins as determined by omics analysis. We propose that this che-associated reduced motility may provide more time for planktonic cells to adhere, also resulting in our observed increase in autoagglutination. This would allow more efficient formation of microcolonies during the initial stages of biofilm formation in C. jejuni (Supplementary Fig. S4) and, thereby, a higher biomass is mature biofilms. All these data combine to suggest that regulation of signal transduction in C. jejuni may be critical during biofilm formation.
C. jejuni biofilms play a functional role in the ability of this organism to withstand environmental stresses and, thereby, impact the ability to infect human hosts. Whilst chemotaxis has been suggested to play an important role, motility has been the main focus in regards to biofilm formation. We have provided evidence that diminished motility is conducive to biofilm formation in C. jejuni, and that chemotaxis may play a larger role than previously thought. This presents the possibility that biofilm formation in C. jejuni may be regulated through modulation of chemotaxis.
Materials and methods
Bacterial strains
The wild-type C. jejuni strain NCTC 11168 O used in this study was provided by Dr. Diane Newell21. ΔcheV and ΔcheW mutant strains were cloned into 11168 O using plasmid constructs provided by Professor Julian Ketley, as described previously16. All strains were grown microaerobically for a period of 12 hrs at 42 °C on Columbia Blood Agar (CBA) with Skirrow supplement, and where appropriate, supplemented with Chloramphenicol (20 µg/mL).
Planktonic cultures of C. jejuni strains for use in proteomic and transcriptomic analysis were grown from an overnight plate culture, which was used to inoculate heart infusion (HI) broth (250 mL) which were incubated under microaerobic conditions at 42 °C and 120 × rpm for 12 hrs. Biofilms were grown from an overnight culture of diluted to an OD600 of 0.75 in Mueller Hinton (MH) broth. The cell suspension (10 mL) was then used to inoculate glass petri dishes and incubated aerobically at 42 °C.
RNA sequencing
Planktonic and biofilm samples of C. jejuni were prepared for transcriptomic analysis as described18.
Briefly, cells were harvested in guanidine thiocyanate (4 M) in a sodium lauryl sarcosine (1%) solution. Samples were carefully laid over a cushion of cesium chloride (5.7 M, 3.5 mL) in EDTA (0.1 M) in an OptiSeal polypropylene centrifuge tube with the remaining volume of the tube being filled with Diethyl pyrocarbonate (DEPC) treated water. Samples were centrifuged at 27,000 x rpm for 16 hrs at 4 °C.
The upper phase was removed and tubes were cut before being allowed to completely drain. The RNA pellet was washed in ethanol (70%, 100 µL) before resuspension in Tris EDTA (TE)/Sodium dodecyl sulfate (SDS; 120 µL) with of TE (80 µL) being used to dissolve remaining RNA before the addition of chilled ethanol (650 µL)/sodium acetate (10 µL). Samples were centrifuged at 14,000 x rpm for 15 mins at 4 °C. The supernatant was removed and ethanol (75%, 650 µL) was used to wash the pellet. Samples were centrifuged for a further 5 mins at 14,000 x rpm and the supernatant removed. Pellets were air dried and reconstituted in RNase free water before being assessed using a Nanodrop 2000.
RNA sequencing analysis was conducted as detailed18.
Preparation of peptide samples for analysis by LC-MS/MS
Following incubation, samples were harvested in RNase free water. Frozen, washed bacterial cell pellets were lyophilised overnight and kept at −80 °C until required. Pellets were resuspended in lysis buffer containing Tris-HCl (150 mM), NaCl (125 mM) and 0.1 mm acid-washed glass beads (Sigma) and lysed by 4 rounds of bead-beating (4 m/s, 1 min) with 1 min rest periods on ice. Cell debris was removed by centrifugation at 16,000 × g for 15 mins at 4 °C. The sample (250 µL) was mixed with ice-cold water/methanol/chloroform in a ratio of 3:4:1 to precipitate proteins. Proteins were resuspended in urea (6 M), thiourea (2 M) and reduced with dithiothreitol (DTT; 10 mM) at 37 °C for 1 hr followed by alkylation with iodoacetamide (IAA; 20 mM) for 1 hr at room temperature in the dark. Samples were then diluted 10-fold in triethylammoniumbicarbonate (TEAB; 100 mM) and quantified using the Qubit protocol (Life Technologies, Carlsbad CA). Samples were digested with trypsin in a ratio of 1:50 enzyme/sample for 16 hrs at 37 °C. Lipids were precipitated using formic acid (FA) and removed by centrifugation at 16,000 x g for 15 mins at 4°C.
Supernatants were acidified with trifluoroacetic acid (TFA) to a final concentration of 0.1%, and peptide purification was performed using 60 cm3 hydrophilic lipophilic balance (HLB) cartridges (Waters Corp., Bedford MA). Cartridges were activated with methanol (100%, 1 volume), followed by acetonitrile (MeCN; 100%, 1 volume) and MeCN (70%)/TFA (0.1%, 1 volume). The cartridges were equilibrated with TFA (0.1%, 2 volumes) and loaded with peptide sample. Samples were reapplied three times to ensure sufficient binding, washed with TFA (0.1%), and eluted with 70% acetonitrile (70%)/TFA (0.1%, 1 volume). Peptides were then lyophilised and resuspended in TEAB (100 mM) and quantified using Qubit. Peptides (125 µg) were labelled per channel using isobaric tags for relative and absolute quantitation (iTRAQ) according to the manufacturer’s protocol (SCIEX, Framingham, MA). Samples were combined and diluted to 1 mL in TFA (0.1%) and purified by HLB columns, as described above. Labelled samples were lyophilized and stored at −80°C until required.
Quantitative LC-MS/MS of peptides fromC. jejuni
iTRAQ-labelled peptides (8 µg) were separated into 10 fractions by offline hydrophilic interaction liquid chromatography (HILIC) using an Agilent 1100 chromatography system. Fractionation was performed using a column (20 cm, 320 µm inner diameter) packed with TSK-Amide 80 HILIC resin, particle size (Tosoh Biosciences; 3 µm). Samples were resuspended in buffer B (90% MeCN/0.1% TFA) and separated using a linear gradient: sample loading for 10 mins with 100% buffer B (100%) at 12 µL/min, sample elution from buffer B (90–60%) at 6 µL/min for 40 mins. Peptide elution was monitored by an absorbance detector at 280 ± 4 nm. Fractionated samples were lyophilised and stored at −20 °C until mass spectrometric analysis.
HILIC fractionated peptides were resuspended in FA (0.1%) and separated on an Ekspert NanoLC 400 system coupled to a SCIEX TripleTOF 6600 quadrupole time-of-flight mass spectrometer (SCIEX, Framingham MA) operated using AnalystTF (v.1.7.1). Peptides were loaded in buffer A (0.1% FA) directly onto an in-house packed 75 µm x 55 cm reversed phase column (1.9 µm particle size, C18AQ; Dr Maisch, Germany), then separated by adjusting the proportion of buffer B (80% MeCN, 0.1% FA) from 5–40% over 120 mins at 400 nL/min at 55 °C. The TripleTOF 6600 was operated in positive ion mode using information (data) dependant acquisition mode, with the top 40 most intense ions with a minimum ion count of 400 and charge state between +2 and +5 selected for MS/MS fragmentation. MS scans were acquired using a mass range of 350–1400 m/z and an accumulation time of 250 ms, with the instrument operated in high sensitivity mode using a mass range of 100–1800 m/z, accumulation time of 50 ms, resolution set to unit, mass tolerance set to 20 ppm and the options for rolling collision energy (CE) and adjust CE for iTRAQ reagent selected.
LC-MS/MS data were identified and quantified with ProteinPilot (5.0.0.0) using the Paragon algorithm, searched against the UniProt C. jejuni NCTC 11168 genome database (UP000000799; organism ID 192222; release May 24, 2018, 1623 proteins) with search parameters as follows: sample type set as iTRAQ 4-plex (Peptide Labeled), cysteine alkylation with IAA, digestion using trypsin allowing one missed cleavage, instrument set as TripleTOF 6600, search effort as thorough and FDR analysis using a detected protein threshold of 0.05. Total reporter ion intensity for each protein was calculated at the protein level, normalised against the summed reporter ion intensity for all proteins including contaminants present in that channel. An average of reporter ion intensity at the protein level was used for downstream analysis. Proteins were included for analysis only if they contained ≥2 identified and quantified peptides. A difference of ≥ +/−1.5 fold was considered differentially abundant between groups.
Post-processing of transcriptomics and proteomics data
Heat map data were generated in Morpheus (https://software.broadinstitute.org/morpheus/). Functional cluster analysis was performed in STRINGdb22.
Assessment of biofilm formation
Plate-grown cultures were harvested with MH Broth (1 mL) and the OD600 of samples was adjusted to 0.75. Bacterial suspension (100 µL) was used to inoculate a 96-well plate, which was then incubated for 48 hrs at 42 °C. Wells were gently washed with of Phosphate Buffered Saline (PBS; 200 µL) and stained with Crystal Violet (CV; 1%, 100 µL) for 15 mins. Wells were washed a further 3 times with PBS (200 µL) and biofilm solubilised with biofilm solvent (100 µL) as previously published23. Absorbance of samples was then assessed at 600 nm. Statistical significance was determined using a paired, two-tailed students t-test.
Soft agar chemotaxis assays
Soft agar chemotaxis assays were conducted as previously described14. Following incubation, cells were harvested in MHB (1 mL) and OD600 adjusted to 0.5. Bacterial suspension (5 µL) was then stabbed into MH plates (0.35%) and incubated for 48 hrs at 42 °C. The diameter of the measured growth from the stab point was measured in mm. All soft agar chemotaxis assays were performed in triplicate.
Time lapse microscopy
Cells were harvested from plates into MHB (1 mL) and adjusted to an OD600 of 0.75. Bacterial suspensions were used to inoculate 24-well plates containing sterilised glass cover slips. Plates were incubated at 42 °C for 45 hrs. At each five-hour timepoint, cover slips were gently washed with PBS and fixed with formalin (5%). Cover slips were then gently washed with of PBS (1 mL) and stained with of CV (500 µL) for 15 mins. CV was then removed and the cover slips were washed three times in PBS before being allowed to air dry and mounted to slides. Samples were analysed using a Nikon Eclipse E600 light microscope and images taken using a Nikon DXM-1200C digital camera and Act- 1 visualisation software.
Autoagglutination assays
Autoagglutination assays were carried out as previously described14. Cells were harvested from incubated plates in PBS (1 mL) and suspensions were diluted to an OD600 of 1.0. The diluted bacterial suspensions (2 mL) were inoculated into 10 mL glass culture tubes and incubated for a period of 24 hours at 42 °C. The culture tubes were then inspected for agglutination of cells and the upper 1 mL was assessed spectrophotometrically at 600 nm.
Accession codes
Data is accessible through the NCBI Gene Expression Omnibus (GEO) Series accession number GSE133783 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE133783).
References
Szymanski, C. M., King, M., Haardt, M. & Armstrong, G. D. Campylobacter jejuni motility and invasion of Caco-2 cells. Infect Immun. 63, 4295–300 (1995).
Wakerley, B. R. & Yuki, N. Guillain-Barré syndrome. Expert Rev Neurother. 15, 847–9 (2015).
Bronowski, C., James, C. E. & Winstanley, C. Role of environmental survival in transmission of Campylobacter jejuni. FEMS Microbiol Lett. 356, 8–19 (2014).
Brown, H. L. et al. Chicken juice enhances surface attachment and biofilm formation of Campylobacter jejuni. Appl Environ Microbiol. 80, 7053–60 (2014).
Cain, J. A. et al. Proteomics reveals multiple phenotypes associated with N-linked glycosylation in Campylobacter jejuni. Mol Cell Proteomics. https://doi.org/10.1074/mcp.RA118.001199 (2019)
Theoret, J. R. et al. The Campylobacter jejuni Dps homologue is important for in vitro biofilm formation and cecal colonization of poultry and may serve as a protective antigen for vaccination. Clin Vaccine Immunol. 19, 1426–31 (2012).
Fields, J. A. & Thompson, S. A. Campylobacter jejuni CsrA mediates oxidative stress responses, biofilm formation, and host cell invasion. J Bacteriol. 190, 3411–6 (2008).
Chandrashekhar, K. et al. Transducer like proteins of Campylobacter jejuni 81-176: role in chemotaxis and colonization of the chicken gastrointestinal tract. Front Cell Infect Microbiol. 5, 46 (2015).
Dwivedi, R. et al. L-fucose influences chemotaxis and biofilm formation in Campylobacter jejuni. Mol Microbiol. 101, 575–89 (2016).
Fredrick, K. L. & Helmann, J. D. Dual chemotaxis signaling pathways in Bacillus subtilis: a sigma D-dependent gene encodes a novel protein with both CheW and CheY homologous domains. J Bacteriol. 176, 2727–35 (1994).
Rosario, M. M., Fredrick, K. L., Ordal, G. W. & Helmann, J. D. Chemotaxis in Bacillus subtilis requires either of two functionally redundant CheW homologs. J Bacteriol. 176, 2736–9 (1994).
Karatan, E., Saulmon, M. M., Bunn, M. W. & Ordal, G. W. Phosphorylation of the response regulator CheV is required for adaptation to attractants during Bacillus subtilis chemotaxis. J Biol Chem. 276, 43618–26 (2001).
Parrish, J. R. et al. A proteome-wide protein interaction map for Campylobacter jejuni. Genome Biol. 8, 130 (2007).
Rahman, H. et al. Characterisation of a multi-ligand binding chemoreceptor CcmL (Tlp3) of Campylobacter jejuni. PLoS Pathog. https://doi.org/10.1371/journal.ppat.1003822 (2014).
Day, C. J. et al. A direct-sensing galactose chemoreceptor recently evolved in invasive strains of Campylobacter jejuni. Nat Commun. 20, 13206 (2016).
Hartley-Tassell, L. E. et al. Identification and characterization of the aspartate chemosensory receptor of Campylobacter jejuni. Mol Microbiol. 75, 710–30 (2010).
Kalmokoff, M. et al. Proteomic analysis of Campylobacter jejuni 11168 biofilms reveals a role for the motility complex in biofilm formation. J Bacteriol. 188, 4312–20 (2006).
Tram, G. et al. RNA Sequencing Data Sets Identifying Differentially Expressed Transcripts During Campylobacter jejuni Biofilm Formation. Microbiol Resour Announc. 9, https://doi.org/10.1128/MRA.00982-19 (2020)
Kim, J., Park, C. & Kim, Y. Role of flgA for Flagellar Biosynthesis and Biofilm Formation of Campylobacter jejuni NCTC11168. J Microbiol Biotechnol. 25, 1871–9 (2015).
Reuter, M., Mallett, A., Pearson, B. M. & van Vliet, A. H. Biofilm formation by Campylobacter jejuni is increased under aerobic conditions. Appl Environ Microbiol. 76, 2122–8 (2010).
Gaynor, E. C. et al. The genome-sequenced variant of Campylobacter jejuni NCTC 11168 and the original clonal clinical isolate differ markedly in colonization, gene expression, and virulence-associated phenotypes. J Bacteriol. 186, 503–17 (2004).
Szklarczyk, D. et al. STRING v11: protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets. Nucleic Acids Res. 47, 607–13 (2019).
Tram, G., Korolik, V. & Day, C. J. MBDS Solvent: An Improved Method for Assessment of Biofilms. Adv Microbiol. 03, 200–204 (2013).
Acknowledgements
The authors would like to thank Professor Julian Ketley (University of Leicester, United Kingdom) for providing the CheV and CheW mutant strains and Dr Dianne Newell for providing access to the C. jejuni strain 11168 O. This work was supported in part by a National Health and Medical Research Council (NHMRC) of Australia Project Grant (APP1106878; to S.J.C.). G.T. & J.A.C was supported by an Australian Postgraduate Award. W.P.K. was supported by a University of Sydney/Faculty of Science Postgraduate Award in Microbial Vaccinology.
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G.T, W.P.K and S.J.C prepared the main manuscript text. G.T and B.M prepared Figs. 3C, 4 and S3. W.P.K, J.A.C and S.J.C prepared Figs. 1, 2, 3A, 3B, S1 and S2. V.K and C.J.D were responsible for project conception and management. All authors reviewed the manuscript.
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Tram, G., Klare, W.P., Cain, J.A. et al. Assigning a role for chemosensory signal transduction in Campylobacter jejuni biofilms using a combined omics approach. Sci Rep 10, 6829 (2020). https://doi.org/10.1038/s41598-020-63569-5
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DOI: https://doi.org/10.1038/s41598-020-63569-5
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