Abstract
Mitochondrial Ca2+ uptake has a key role in cellular Ca2+ homeostasis. Excessive matrix Ca2+ concentrations, especially when coincident with oxidative stress, precipitate opening of an inner mitochondrial membrane, high-conductance channel: the mitochondrial permeability transition pore (mPTP). mPTP opening has been implicated as a final cell death pathway in numerous diseases and therefore understanding conditions dictating mPTP opening is crucial for developing targeted therapies. Here, we have investigated the impact of mitochondrial metabolic state on the probability and consequences of mPTP opening. Isolated mitochondria were energised using NADH- or FADH2-linked substrates. The functional consequences of Ca2+-induced mPTP opening were assessed by Ca2+ retention capacity, using fluorescence-based analysis, and simultaneous measurements of mitochondrial Ca2+ handling, membrane potential, respiratory rate and production of reactive oxygen species (ROS). Succinate-induced, membrane potential-dependent reverse electron transfer sensitised mitochondria to mPTP opening. mPTP-induced depolarisation under succinate subsequently inhibited reverse electron transfer. Complex I-driven respiration was reduced after mPTP opening but sustained in the presence of complex II-linked substrates, consistent with inhibition of complex I-supported respiration by leakage of matrix NADH. Additionally, ROS generated at complex III did not sensitise mitochondria to mPTP opening. Thus, cellular metabolic fluxes and metabolic environment dictate mitochondrial functional response to Ca2+ overload.
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Introduction
Mitochondria are capable of oxidising numerous substrates based on availability and metabolic demand. The delivery of energetic substrates to mitochondria provides reducing equivalents required for serial reduction of electron transport chain (ETC) redox centres. These redox reactions are coupled to expulsion of protons from the matrix into the intermembrane space (IMS)1. The resulting proton electrochemical gradient (Δp), comprising a membrane potential (ΔΨm) and pH gradient, is necessary for the production of adenosine triphosphate (ATP) and metabolite transport through the inner mitochondrial membrane (IMM)2, 3.
The functions of mitochondria extend beyond that of cellular ATP biosynthesis. Indeed, mitochondria participate in multiple regulatory signalling pathways stimulated in response to both physiological and pathophysiological stimuli. As key regulators of cell death pathways, mitochondria also play a critical role in determining cell fate4, 5. Thorough understanding of the (patho)physiological conditions mediating these homeostatic outcomes is important to help develop new therapeutic agents for a number of diseases including Parkinson’s Disease and stroke6,7,8.
Mitochondrial Ca2+ uptake plays an important role in cellular homeostasis, being driven by the maintenance of ΔΨm 5, 9. The mitochondrial permeability transition pore (mPTP) is a presumed proteinaceous entity in the IMM. Pore opening has generally been attributed to a structural change in a protein embedded within the membrane, which, under other conditions, seems to usually perform a physiological role10, 11. The precise molecular composition and identity of the mPTP is highly controversial but candidates include the adenine nucleotide translocase (ANT), the voltage dependent anion channel (VDAC), spastic paraplegia 7 (SPG7), phosphate carrier (PiC) and components of the ATP synthase12,13,14,15,16,17. Recent observations have further complicated structural understanding of the mPTP complex in that He el al., challenging previous claims15, 18 recently demonstrated intact permeability transition in the absence of membrane and C-ring subunits of the ATP synthase19.
To date, the only unequivocally identified component of the pore complex is cyclophilin D (CypD), a matrix cyclophilin that regulates pore opening, conferring sensitivity of the pore to inhibition by cyclosporin A (CsA)20, 21. Failure of cellular Ca2+ homeostasis and consequent mitochondrial Ca2+ overload is the principal trigger for mitochondrial mPTP opening22. High-conductance mPTP opening is associated with osmotic swelling, loss of IMM potential, uncoupling of oxidative phosphorylation and metabolic collapse4, 22,23,24,25.
As mPTP opening is a determinant of cell death in an ever-growing list of diseases, it is important for clinical benefit to understand pathways culminating in pore opening to identify effective interventions preventing or limiting pore opening26,27,28,29. To explore the impact of different metabolic conditions on the probability of pore opening, fluorescence-based analysis coupled to high-resolution respirometry was used to simultaneously measure mitochondrial Ca2+ uptake, ΔΨm, reactive oxygen species (ROS) production and respiration. The threshold of mPTP opening was assessed under distinct metabolic conditions by measuring the Ca2+ buffering capacity of isolated mitochondria. Characterisation of the relationship between substrate utilisation, oxygen consumption and sensitivity to pore opening will reveal events that precede and follow mPTP opening. Here, we report variation in mitochondrial sensitivity to Ca2+-induced mPTP opening based on the source of electron flux through the respiratory chain, as driven by different metabolic substrates, identifying mechanisms by which energetic state determines the mitochondrial response to Ca2+ overload.
Results
Influence of mitochondrial metabolic substrate availability on mPTP opening
Mitochondria are capable of metabolising several different metabolic substrates. To understand how substrate availability determines the probability of mPTP opening, a Ca2+ retention capacity protocol adapted to simultaneously monitor multiple parameters was established using the FLIPRTETRA high content kinetic assay system. Using this assay, mPTP opening was identified as the release of mitochondrially sequestered Ca2+, the failure of mitochondrial Ca2+ uptake (measured using the low affinity calcium indicator Fluo-4FF) and the collapse of the mitochondrial membrane potential (measured using TMRM).
Mitochondrial suspensions were energised using mechanistically distinct substrate combinations: (1) glutamate/malate, (2) succinate or (3) succinate/rotenone. Under all metabolic conditions, initial aliquots of added Ca2+ (10 µM) were effectively removed from the extra-mitochondrial solution by mitochondrial Ca2+ uptake (Fig. 1a: insert 1) with no measurable effect on ΔΨm (Fig. 1b). Greater Ca2+ loads (concentrations above a threshold) caused mPTP opening and simultaneous collapse of ΔΨm under all metabolic conditions (Fig. 1a: insert 2 and Fig. 1b). However, strikingly, mitochondria oxidising glutamate/malate tolerated significantly greater Ca2+ loads, compared to those oxidising succinate and succinate/rotenone (Fig. 1a and c) which began to deviate from the glutamate/malate condition following 3–4 injections of calcium (Fig. 1e). A decrease of ΔΨm occurred significantly earlier in mitochondria energised with glutamate/malate compared to those metabolising succinate (Fig. 1b and d): deviation from the succinate condition occurred following just 1–2 calcium additions and prior to overt mPTP opening (as judged by Ca2+ retention assay; Fig. 1f). Addition of the complex I inhibitor rotenone to succinate-energised mitochondria significantly delayed mPTP opening over controls (Fig. 1c). Cyclosporin A (CsA) is a potent inhibitor of cyclophilin D (CypD), the well-established blocker of mPTP opening30. Under all metabolic conditions tested, mPTP opening was delayed by CsA and ΔΨm was maintained (Fig. 1a,b, Supplementary Figure 1). Independent of the metabolic substrate employed, CsA caused a dose-dependent delay in mPTP opening. IC50 values for CsA between substrates ranged from 86–92 nM (Table 1 and Supplementary Figure 1). Together, these data suggest that mitochondrial substrate utilisation influences the threshold required for Ca2+ to induce mPTP opening and has no effect of CsA potency of action.
Effect of metabolic substrate availability on bioenergetic consequences of mPTP opening
We next aimed to understand the consequences of the loss of mitochondrial inner membrane integrity on respiratory chain electron flow and to explore how metabolic substrate selectivity influences the bioenergetic consequences of mPTP opening. Simultaneous recordings of respiration, ΔΨm and Ca2+ uptake were performed in isolated mitochondria in response to Ca2+ overload, using the Oroboros Oxygraph 2 K high resolution respirometry system coupled with a fluorescence detection unit.
Independent of respiratory substrate, additions of sub-threshold Ca2+ concentrations resulted in complete mitochondrial Ca2+ uptake (Green traces; Fig. 2a: insert-1, Fig. 2c,e). Prior to mPTP opening, additions of Ca2+induced an increase in respiration in mitochondria oxidising any of the substrates (Blue traces; Fig. 2a–f). Importantly, upon mPTP opening (i.e. the point at which Fluo-4FF fluorescence failed to return to baseline, (Fig. 2a: insert-2), the respiratory rate supported by NADH-linked substrates glutamate/malate decreased (Fig. 2a,b), while FADH2-driven mitochondrial respiration remained at an elevated plateau (Fig. 2c–f).
TMRM was used to assess changes in ΔΨm in parallel to measurement of respiration. In glutamate/malate energised mitochondria, transmembrane depolarisation in response to Ca2+ additions coincided with decreased respiration (Fig. 2b). Inhibition at cytochrome bc 1 of mitochondrial complex III using antimycin A had no further effect on the TMRM signal, suggesting that the membrane was already completely depolarised (Red trace; Fig. 2b). In contrast, using the FADH2-linked substrate succinate (±rotenone), ΔΨm loss occurred after a Ca2+-induced increase in respiration, remaining sustained at an elevated respiratory rate under these conditions (see above, Fig. 2c–f). Together, these data suggest that the mitochondrial respiratory response and the kinetics of ΔΨm loss in response to mPTP opening alter depending on metabolic substrate availability. Transient stimulation of respiration was followed by decreased respiration using NADH-linked substrates while, in contrast, oxidation of FADH2-linked substrates resulted in a large Ca2+-induced increase in mitochondrial oxygen consumption, sustained during mPTP opening and consistent with both an uncoupling response and a slower rate of mitochondrial depolarisation.
Respiratory consequences of mitochondrial Ca2+ load are primarily driven by mPTP
To investigate whether the effects of increasing mitochondrial Ca2+ load on mitochondrial respiration were triggered directly by Ca2+ or indirectly by mPTP opening, we used either ruthenium red (RuR) or CsA to, respectively, block mitochondrial Ca2+ uptake or delay mPTP opening. Mitochondria were incubated with glutamate/malate and subjected to a Ca2+ retention capacity assay. Consistent with published data31,32,33, we observed a small but significant decrease in State 3 respiration using both CsA and RuR (Fig. 3a). Acute incubation with CsA delayed mPTP opening, mitochondria tolerated greater Ca2+ loads (Fig. 3b) and both the associated decline in respiration and collapse of ΔΨm were prevented (Fig. 3b,c). Importantly, inhibition of mitochondrial Ca2+ uptake by RuR had the same effect on both respiration and ΔΨm (Fig. 3d,e), confirming the dependence of mPTP opening on mitochondrial Ca2+ uptake. Also, the Ca2+-induced increase in respiration prior to mPTP opening was completely prevented not only by blocking mitochondrial Ca2+ uptake (RuR) but also by preventing mPTP opening via CypD inhibition (CsA; Fig. 3b–e). This suggested the increase in respiration was due to Ca2+-induced, CsA-sensitive inner membrane uncoupling which could, however, at least to the point of mPTP opening, be compensated for through increased respiration.
Loss of complex I-driven respiration after mPTP opening can be rescued through addition of exogenous NADH or succinate
Next we explored the mechanism behind the respiratory deficit after mPTP opening in NADH-driven respiration. Addition of exogenous succinate following mPTP opening in glutamate/malate energised mitochondria caused a rapid increase in antimycin A-sensitive respiration without affecting TMRM or extra-mitochondrial [Ca2+] (Fig. 4a). Addition of exogenous NADH also significantly increased the respiratory rate (Fig. 4b). Together, these data suggest that either feeding electrons into complex II (bypassing complex I) or adding saturating concentrations of NADH support respiration following mPTP opening. These findings confirm the respiratory deficits are a consequence of the loss of soluble, diffusible reducing equivalents - primarily NADH, from the tricarboxylic acid (TCA) cycle via mPTP opening, while the provision of reducing equivalents at complex II, or addition of exogenous NADH are sufficient to maintain respiratory capacity.
Substrate selectively dictates rates of free radical generation both before and after mPTP opening
Mitochondria are an important source of cellular ROS, also implicated in sensitising mPTP formation to Ca2+ 10, 34. Thus in order to dissect the relationship between metabolic substrates, ROS generation, respiratory chain activity, mitochondrial Ca2+ load and mPTP opening, we measured ROS generation before and after Ca2+ induced mPTP opening. The primary radical species generated by mitochondria is superoxide35,36,37. Although superoxide cannot cross membranes, it is rapidly converted to H2O2 by matrix-localised superoxide dismutase (MnSOD) or spontaneously dismutates at a high rate. Additionally, H2O2 can be directly produced by some mitochondrial sites (i.e. flavins38). Therefore, we measured H2O2 generated by isolated mitochondria to assess overall rates of mitochondrial ROS (mtROS) generation.
Extra-mitochondrial Ca2+ concentration (Fluo-4FF) and H2O2 production (Amplex Red; AmpR) were measured simultaneously in a Ca2+ retention assay protocol. The rate of baseline H2O2 production by mitochondria energised using glutamate/malate was significantly higher than that generated by succinate alone (Fig. 5a). Rotenone significantly decreased succinate-driven mtROS production, suggesting that H2O2 production in the presence of succinate originates from superoxide generated by reverse electron transfer (RET) from complex II to complex I39,40,41. Overall, however, the contribution of RET to the total AmpR signal was low (~10%), and the resulting feed forward rates of mtROS flux in succinate/rotenone-driven respiration were significantly lower than in mitochondria energised by glutamate/malate (Fig. 5a), indicating that NADH-driven forward electron flow through complexes I and III are significant sources of mtROS generation. Importantly, no relationship between rates of mtROS production and Ca2+ sensitivity of mPTP opening was observed when supported by complex I-linked substrates, as higher rates of mtROS generation supported by glutamate/malate-driven respiration (Fig. 5a) were not associated with a higher probability of Ca2+ induced mPTP opening (see Fig. 1a). However, in contrast, the reduction in baseline mtROS production by the addition of rotenone under succinate-driven respiration (Fig. 5a) reduced the probability of mPTP opening (see Fig. 1a). These results suggest that reverse electron flow through complex I contributes significantly to sensitisation of mPTP opening, while forward electron flow through complexes I and III does not affect the process.
In both glutamate/malate- and succinate/rotenone-energised mitochondria, mPTP opening was preceded by a Ca2+-induced increase in H2O2 production (Fig. 5b). This accompanied the Ca2+-dependent increased respiration (forward electron flow) which was attributed to Ca2+-mediated, CsA-sensitive transient uncoupling (see above). In contrast, although a Ca2+-induced sustained increase in respiratory rate was supported by succinate (see Fig. 1c,d), this was accompanied by only a short transient increase in H2O2 production which preceded mPTP opening (Fig. 5b). Importantly, CsA and RuR inhibited mtROS production in a dose-dependent manner with glutamate/malate and succinate/rotenone as substrates, while both inhibitors increased rates of mtROS production when mitochondria were respiring only on succinate (Fig. 5c,d,e and Supplementary Figure 2). These data suggest that the loss of ΔΨm due to mPTP opening has a differential impact on the forward (glutamate/malate and succinate/rotenone) and reverse (succinate alone) electron flows. Indeed, in healthy mitochondria (before mPTP opening), the rate of H2O2 production following uncoupling with FCCP was: (i) dose-dependently reduced when mitochondria were respiring on succinate alone and; (ii) dose-dependently increased when using glutamate/malate as substrates (Fig. 5f), confirming the differential ΔΨm dependence of mtROS production.
To further establish the relationship between mtROS production and respiration, the rates of oxygen consumption, Ca2+ uptake and H2O2 production were measured simultaneously using NADH-linked substrates. The Ca2+-induced transient increase in respiratory rate preceding permeability transition correlated with an increased AmpR fluorescence (Fig. 5g and h), and both CsA and RuR prevented the Ca2+-induced respiratory changes, maintaining the rates of H2O2 production at baseline levels (Fig. 5g and h).
Finally, in order to verify the sites of mtROS production by the respiratory chain, the rates of H2O2 production were compared across conditions following mPTP opening (i.e. after 10 sequential CaCl2 additions) and complex III inhibition on the FLIPRTETRA high content kinetic assay system. Antimycin A stimulated a large CsA- and RuR-sensitive increase in H2O2 production across all substrates (Fig. 6a–c). These responses were greater than those observed in control mitochondria (without Ca2+ stimulation), suggesting that rates of mtROS production from complex III are augmented by mPTP opening (Supplementary Figure 2). In contrast, rotenone did not increase H2O2 production in the presence of any tested substrate in this study (Fig. 6a–c), arguing against significant mtROS generation by forward flow through complex I. Taken together, these data show that respiratory substrates, by sustaining reverse or forward electron flow, determine the consequences of Ca2+-induced changes in mtROS production and the relationship between ΔΨm and respiratory rate ultimately determines the rate of mtROS generation.
Discussion
We have investigated the effects of mitochondrial substrate availability on the probability of Ca2+-induced mPTP opening and its consequences. We observed different changes in mitochondrial respiration, membrane potential and free radical generation as a function of Ca2+ loading and mPTP opening under distinct metabolic conditions. In particular, the simultaneous measurements of combinations of respiratory rate, Ca2+ uptake, IMM potential and mtROS generation have generated new insights into the specific relationships between thee variables. Our key findings were: (i) the probability of mPTP opening is increased when respiration is supported by succinate compared to glutamate and malate, a phenomenon reduced by the presence of rotenone; (ii) the efficiency of succinate to induce mPTP opening correlates with succinate-induced RET through complex I and associated mtROS production, (iii) mPTP-induced depolarisation inhibits RET from succinate energised mitochondria; (iv) in the presence of succinate, respiration is maintained following mPTP opening and loss of mitochondrial membrane potential; (v) when respiration is supported by glutamate/malate, mPTP opening causes a transient increase in respiration followed by a decrease that can be restored by addition of NADH or succinate, consistent with respiratory inhibition due to the loss of matrix NADH; and (vi) although ROS are generated from complex III in the presence of both glutamate/malate and succinate/rotenone, this does not sensitise mPTP opening.
Previously, Huang et al. demonstrated that mPTP opening and subsequent cytochrome c release are more sensitive to Ca2+ when mitochondria are oxidising FADH2-linked rather than NADH-linked substrates42. We have added to these observations to understand metabolic consequence of Ca2+-induced mPTP opening, investigating bioenergetic changes, mitochondrial Ca2+ uptake, ΔΨm and mtROS generation. We have confirmed that respiration supported by succinate sensitises mitochondria to Ca2+-induced mPTP opening. Inhibition of electron flux from complex I to ubiquinone (UQ) by rotenone decreased both baseline mtROS production and the efficiency of succinate-energised mitochondria to undergo mPTP opening. This suggests that ROS generation through complex I may define the sensitivity of mitochondria to Ca2+-induced mPTP opening under these conditions. It is noteworthy that reperfusion following ischemia (high ΔΨm, reduced UQ), has been associated with succinate accumulation and increased ROS generation by RET39, 40, 43. Prevention of Ca2+-induced RET using rotenone reduces oxidative stress in succinate-energised mitochondria, likely being responsible for the decreased mitochondrial sensitivity to Ca2+-induced mPTP opening. In agreement with Chouchani et al. reverse flow of electrons through complex I contribute to mPTP opening43. Additionally, we have also determined that ROS production through reverse flow is membrane potential-dependent and thus must precede mPTP opening.
We observed only a transient increase in Ca2+-induced mtROS production in succinate-energised mitochondria. Since high ΔΨm is necessary to drive RET and the reduction of NAD+, the observed Ca2+-induced decrease in mtROS production following mPTP opening and membrane depolarisation is likely due to the cessation of RET41, 44,45,46. Consistent with these observations, addition of the ETC uncoupler, FCCP to intact/healthy mitochondria also dose-dependently decreased mtROS production in mitochondria energised by succinate alone. In succinate-energised mitochondria, the FCCP-mediated decrease in mtROS production was observed at concentrations consistent with membrane depolarisation and increased oxygen consumption47, responses analogous to mPTP opening. Additionally, inhibition of mPTP opening, using CsA and RuR, prevented the Ca2+-induced decrease in mtROS, suggesting that mPTP opening following Ca2+ uptake contributes to decreased oxidative stress in mitochondria metabolising succinate alone. mPTP-induced depolarisation therefore inhibits RET and mtROS generation from succinate (similar to FCCP), correlating with a transient increase in ROS in ‘succinate only’-energised condition and suggesting a transient peak in ROS can be efficient in pre-sensitising mPTP to Ca2+.
Despite Complex I-linked substrates generating higher mtROS at baseline, no increased sensitivity to Ca2+ -induced mPTP opening was observed. However, addition of rotenone under succinate-driven respiration reduced the probability of mPTP opening, suggesting that RET through complex I sensitises to mPTP opening, while forward electron flow through Complexes I and III does not affect the process. As expected, we observed an antimycin A-dependent increase in baseline mtROS production. Electron carriers upstream of the point of antimycin A-sensitive inhibition become reduced causing a large increase in baseline H2O2 production in mitochondria metabolising all substrates48. The antimycin A-dependent increase in H2O2 production is both greater after mPTP opening and sensitive to CsA and RuR, suggesting mPTP opening mediates the augmented mtROS production. Together, these observations suggest that complex III appears the major source of mtROS production following mPTP opening and, although glutamate/malate and succinate/rotenone produce ROS on complex III, it is not a sensitising factor for stimulating mPTP opening. ROS may have differential effects when generated at the inner or outer face of the mitochondrial inner membrane. Superoxide generation from complex I is directed towards the matrix and complex III to both the matrix and IMS, suggesting that complex I is the most significant source of superoxide generation towards the mitochondrial matrix49, 50. As superoxide does not cross membranes and is extremely reactive, it will initially react with components that are exposed in the environment next to the site of generation51, 52. Microdomains therefore are highly relevant for ROS species as they have a limited diffusion range due to their high, localised reactivity.
Under all metabolic conditions, rates of mitochondrial oxygen consumption increased following initial additions of Ca2+ preceding mPTP opening. Ca2+-induced stimulation of respiration has been proposed to occur through different mechanisms. Increased NADH production by Ca2+ sensitive dehydrogenases (pyruvate and oxoglutarate) of the TCA cycle53, Ca2+-mediated stimulation of succinate dehydrogenase54, or dissipation of the H+ gradient by futile Ca2+ cycling could augment electron flow through the respiratory chain55. However, under our conditions the Ca2+-induced respiratory increase was fully blocked both by CsA and RuR. Thus, we hypothesise that low-conductance, reversible mPTP opening, may be responsible for the respiratory increase observed following sub-threshold Ca2+ uptake56, 57. Given the absence of effect of low concentration Ca2+ on ΔΨm, it is likely that these mild uncoupling events can be compensated for through increased respiration, as observed previously using FCCP at low concentration47. The lack of effect of Ca2+ on NADH provision to complex I may reflect that principal sites of NADH production using glutamate/malate are the Ca2+-insensitive glutamate and malate dehydrogenases and, additionally, that succinate dehydrogenase under these conditions has been shown to be inactivated by oxaloacetate58, 59.
High-conductance mPTP opening is consistent with mitochondrial inner membrane uncoupling and loss of ΔΨm. In contrast to succinate-energised mitochondria, where respiration is maintained following mPTP opening, in glutamate/malate-energised mitochondria, respiratory rates decreased following Ca2+-induced high-conductance mPTP opening. Soluble NAD+/NADH is lost through the mPTP to the extra-mitochondrial space, limiting electron flux from UQ to complex III60. mPTP opening and respiratory decline were prevented by CsA and RuR as soluble reducing equivalent are preserved within the matrix. Intriguingly, addition of exogenous NADH or succinate following complete mPTP opening and inner membrane depolarisation is able to recover and increase respiration. These observations are consistent with those following Ca2+ overload and mPTP opening in succinate-driven respiration. The flavoprotein pool, via which electrons pass from succinate, is inner membrane bound, therefore facilitating maintenance of respiration as succinate saturates the pool and respiratory rate hits maximum. In contrast to respiration driven by glutamate/malate at complex I, where soluble respiratory intermediate NADH is lost from the matrix. Previously, Hawkins et al. observed that supplementation by succinate could prevent rotenone/oligomycin- and hypoxia-induced mitochondrial depolarisation. In agreement with the data presented here, succinate failed to maintain ΔΨm following mPTP opening61.
In summary, we investigated the role of substrate selectivity and utilisation in determining propensity for Ca2+-induced mPTP opening. We have characterised key modulatory factors, including mitochondrial energetic status and Ca2+ concentration in both regulating propensity for mPTP opening and determining consequence of pore opening. Together, these data further add to understanding the patho(physiological) effects of pore opening. For decades, mPTP opening has been recognised as a critical factor in cellular death across numerous diseases. Therefore, understanding the precipitating factors, the pathways culminating in pore opening and the downstream metabolic consequences will aid the rational design and development of therapeutics, with potential utility in treating multiple difficult-to-treat disease states.
Materials and Methods
Materials
All chemicals and compounds were purchased from Sigma-Aldrich (St. Louis, MO), unless otherwise specified. Fluorescent probes (tetramethylrhodamine methylester perchlorate; TMRM, Fluo-4FF, Amplex Red™; AmpR) were from Life Technologies (Eugene, OR).
Animals
Female Sprague Dawley rats (250–300 g) were from Charles River (Wilmington, MA) and allowed to acclimatise to conditions for four days. Animals were euthanised by cervical dislocation followed by immediate removal of livers. Animal care and procedures were performed in accordance with UK Animals (Scientific Procedures) Act, 1986. Procedures were carried under a UK Home Office licence and studies were approved by Eisai’s Institutional Animal Care and Use Committee (IACUC).
Isolation and storage of mitochondria
Mitochondria were isolated in accordance with published protocols62. Briefly, fresh rat livers were washed and finely minced in ice-cold wash buffer (250 mM sucrose, 10 mM KCl, 1 mM EGTA, 1 mM EDTA, 25 mM HEPES, adjusted to pH 7.5 using NaOH). Tissue was homogenised using 10 strokes of a glass/Teflon potter and drill, set to 1600 rpm, in 5x tissue volume of complete homogenisation buffer (300 mM trehalose dihydrate, 25 mM HEPES, 1 mM EGTA, 1 mM EDTA, 10 mM potassium chloride, 0.1% essentially fatty acid free bovine serum albumin (BSA) (Sigma, A3803), cOmplete Protease Inhibitor™ (Roche Diagnostics, Mannheim, Germany), adjusted to pH 7.5). Homogenates were centrifuged at 800 g for 10 minutes at 4 °C, supernatants transferred to a clean tube and then centrifuged further at 10,300 g at 4 °C for 10 minutes. Mitochondrial pellets were surface-washed using complete homogenisation buffer and the final centrifugation step repeated. The pellets were re-suspended in complete homogenisation buffer and protein concentration determined by bicinchoninic acid assay (BCA) (Thermo Scientific, Rockford, IL). Mitochondrial suspensions (50 mg protein ml−1) were snap-frozen in liquid nitrogen and stored at −80 °C until use. All mitochondrial preparations were maintained at −80 °C for up to 7 months. Prior to activity assays, frozen mitochondria were thawed by briefly placing vials in a 37 °C water bath and then kept on ice until required.
Ca2+ retention capacity (CRC) assay using FLIPRTETRA
Assessment of Ca2+ retention capacity was used to assess in vitro sensitivity to Ca2+ of isolated mitochondrial preparations. Mitochondria were washed in ice-cold mitochondrial assay buffer (MAB; 75 mM mannitol, 25 mM sucrose, 5 mM potassium phosphate monobasic, 20 mM Tris base, 100 mM potassium chloride, 0.1% bovine serum albumin, adjusted to pH 7.4) to remove residual EDTA and re-suspended (2 mg protein ml−1, final assay concentration (FAC) = 1 mg protein ml−1) in complete MAB. To remove any contaminating Ca2+, MAB was pre-treated with Chelex 100 resin (Sigma-Aldrich, St. Louis, MO) and resin removed through filtration.
Complete MAB containing 2x Fluo-4FF penta-potassium salt (0.7 μM, FAC = 0.35 μM) was supplemented with either: (1) 20 mM L-glutamic acid, monosodium salt, FAC = 10 mM; 4 mM L-malic acid sodium salt, FAC = 2 mM, (2) 20 mM L-glutamic acid monosodium salt, FAC = 10 mM; 4 mM L-malic acid sodium salt, FAC = 2 mM; 6 mM NADH, FAC = 3 mM, (3) 20 mM succinate disodium salt, FAC = 10 mM or (4) 20 mM succinate disodium salt, FAC = 10 mM; 2 μM rotenone, FAC = 1 μM). Final pH of the solutions was confirmed to be 7.4 and adjusted where necessary using NaOH.
Mitochondrial suspensions (2x concentration; 20 μl) and supplemented Fluo-4FF containing MAB (2x concentration; 20 μl) were dispensed into a clear-bottom, black-walled 384 well plate containing compound using a Multidrop Combi Reagent Dispenser (Thermo Scientific, Rockford, IL) and incubated for 10 mins at room temperature. Extra-mitochondrial fluorescence (ex. 470–495/em. 515–575) was measured at 6 second intervals (FLIPRTETRA, Molecular Devices, Sunnyvale, CA) over 35 minutes at room temperature. CaCl2 (10 μM final concentration per addition) in MAB (2.5 μl additions to 40 μl) was repeatedly added at 3 minute intervals.
Ca2+-induced mitochondrial membrane depolarisation using FLIPRTETRA
Mitochondrial ΔΨm was measured using tetramethylrhodamine methylester (TMRM), a voltage-sensitive cationic lipophilic dye, partitioning and accumulating in the mitochondrial matrix based upon the Nernst equation. When TMRM is loaded at relatively high concentrations (>100 nM63), fluorescence within the mitochondria is auto-quenched. Any disruption to mitochondrial function (e.g. membrane uncoupling or electron transport inhibition) dissipates ΔΨm with TMRM redistribution yielding an increase in fluorescence upon relief of auto-quenching.
To remove residual EDTA, mitochondria were centrifuged at 10,300 g at 4 °C for 10 minutes and re-suspended in ice-cold complete MAB to a final concentration of 2 mg protein ml−1 (final assay concentration (FAC) = 1 mg protein ml−1). MAB was pre-treated with Chelex 100 resin (Sigma-Aldrich, St. Louis, MO) to remove any contaminating Ca2+, and resin removed through filtration. MAB containing TMRM (2 μM, final assay concentration = 1 μM) was supplemented as for CRC assay. Mitochondrial suspension (2x concentration; 20 μl) and supplemented TMRM containing MAB (2x concentration; 20 μl) were dispensed into a clear-bottom, black-walled 384 well plate containing the compounds using a Multidrop Combi Reagent Dispenser (Thermo Scientific, Rockford, IL) and incubated with the compounds for 10 mins at room temperature to allow TMRM equilibration. Fluorescence (ex. 510–545/em. 565–625) was measured at 6 second intervals (FLIPRTETRA, Molecular Devices, Sunnyvale, CA) over 35 minutes at room temperature. CaCl2 (10 μM final concentration per addition) in MAB (2.5 μl additions to 40 μl) was repeatedly added at 3 minute intervals.
Measurements of hydrogen peroxide generation using FLIPRTETRA
H2O2 production was determined using Amplex Red (AmpR), a dye reacting with 1:1 stoichiometry with H2O2 in a peroxidase-catalysed reaction to produce the highly fluorescent resorufin. Mitochondria were washed in ice-cold MAB to remove residual EDTA and re-suspended (2 mg protein ml−1, final assay concentration (FAC) = 1 mg protein ml−1) in complete MAB. MAB was pre-treated with Chelex 100 resin (Sigma-Aldrich, St. Louis, MO) to remove any contaminating Ca2+, and resin removed through filtration.
MAB containing 2x AmpR (20 μM) and horseradish peroxidase (HRP; 2 U ml−1) was supplemented as above for CRC assay. Mitochondrial suspension (2x concentration; 20 μl) and supplemented AmpR/HRP containing MAB (2x concentration; 20 μl) were dispensed into a clear-bottom, black-walled 384 well plate containing compound using a Multidrop Combi Reagent Dispenser (Thermo Scientific, Rockford, IL) and incubated with compound for 10 mins at room temperature. Fluorescence (ex. 510–545/em. 565–625) was measured at 6 second intervals (FLIPRTETRA, Molecular Devices, Sunnyvale, CA) over 35 minutes at room temperature. CaCl2 (10 μM final concentration per addition) in MAB (2.5 μl additions to 40 μl) was repeatedly added at 3 minute intervals.
High-resolution respirometry and parallel fluorescence measurements
Mitochondrial oxygen consumption was measured using the Oxygraph 2 K (Oroboros Instruments, Innsbruck, Austria). Mitochondria were washed in ice-cold MAB, to remove residual EDTA. Mitochondria were re-suspended (1 mg protein ml−1) in MAB supplemented with either: (1) 10 mM L-glutamic acid, monosodium salt; 2 mM L-malic acid sodium salt, (2) 10 mM succinate disodium salt or (3) 10 mM succinate disodium salt; 1 μM rotenone. Mitochondrial suspensions were added to each chamber, maintained at 25 °C under constant stirring (250 rpm), Oxygraph 2 K calibrated using oxygen solubility factor 0.92 and respiration allowed to stabilise.
The Oxygraph 2 K was equipped with an O2K fluorimeter and fluorescence control unit (Oroboros Instruments, Innsbruck, Austria) and TMRM (2 μM), Fluo-4FF (1 μM) and Amplex Red/HRP (10 μM/1 U ml−1) were added to chambers for parallel measurement of ΔΨm, extracellular Ca2+ and H2O2 respectively. A sequential titration protocol, including additions of 10 mM succinate disodium salt, 1 μM rotenone, 2.5 μM antimycin A, 0.5 μM carbonyl cyanide p-[trifluoromethoxy]-phenyl-hydrazone (FCCP) and 3 mM NADH disodium salt, 0.5 μM CsA and 0.5 μM RuR were added, as indicated in results. Extra-mitochondrial Ca2+ fluorescence (fluorescence-sensor blue; LED ex. 465), ΔΨm and H2O2 (both fluorescence-sensor green; LED ex. 525) were measured in parallel to respiration at 3 second intervals over the assay duration. CaCl2 (2.5 μM final concentration per addition) in MAB (2 μl additions to 2 ml) was repeatedly added at 1.5 minute intervals.
Experimental design, data analysis and statistical procedures
Data are presented as mean ± standard deviation (s.d.). Normalisation of data allowed for control of inter-assay variability. AUC was calculated using ScreenWorks (Molecular Devices, Sunnyvale, CA). Curve fitting used GraphPad Prism version 7.02 for Windows (La Jolla, California, USA). Statistical significance tests performed are indicated in figure legends. Statistical significance was determined as P < 0.05.
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This work was funded as part of the UCL: Eisai Drug Discovery and Development Collaboration Agreement.
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T.B. designed experiments, performed experiments and wrote the manuscript. M.R.D. and G.S. designed experiments, provided scientific input, project leadership and wrote the manuscript. M.R. and S.L. generated mitochondria. J.S., M.R. and B.P. provided scientific input and project leadership.
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Briston, T., Roberts, M., Lewis, S. et al. Mitochondrial permeability transition pore: sensitivity to opening and mechanistic dependence on substrate availability. Sci Rep 7, 10492 (2017). https://doi.org/10.1038/s41598-017-10673-8
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DOI: https://doi.org/10.1038/s41598-017-10673-8
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