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Prophylactic effect of rosmarinic acid on tracheal responsiveness, white blood cell count and oxidative stress markers in lung lavage of sensitized rats

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Abstract

Background

Rosmarinic acid (RA) as an active component of several medicinal plants, has shown anti-inflammatory and anti-oxidant effects. In this study, the effect of RA on tracheal responsiveness (TR), lung inflammatory cells, oxidant biomarkers in sensitized rats were evaluated.

Methods

TR to methacholine and ovalbumin (OVA) as well as total and differential white blood cell (WBC) count and levels of nitrogen dioxide, nitrate, malondialdehyde, thiol, superoxide dismutase, and catalase in bronchoalveolar lavage fluid were measured in control (group C) rats, sensitized animals to OVA and given drinking water alone (group S), S groups receiving drinking water containing three concentrations of RA (0.125, 0.250 and 0.500 mg/mL) and dexamethasone (1.25 μg/mL), (n = 6 in each group).

Results

Increased TR to methacholine and OVA, total WBC count, percentages of eosinophils, monocytes, neutrophils and levels of oxidant biomarkers but decreased other measured parameters were observed in group S compared to group C. Percentages of lymphocytes and antioxidant biomarkers were significantly increased but other measured parameters were significantly decreased in S group treated with dexamethasone and in rats treated with the two higher concentrations of RA compared to S group. The effect of RA medium concentration on percentage of eosinophils and RA high concentration on total WBC count and percentages of eosinophils and lymphocytes, were significantly higher than those of dexamethasone.

Conclusion

These results showed the concentration-dependent effect of RA on tracheal responses, lung inflammatory cells and oxidant-antioxidant parameters which was comparable to that of dexamethasone at used concentrations in sensitized rats.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

    Naeima Eftekhar & Ali Moghimi

  2. Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Hossein Boskabady

  3. Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Hossein Boskabady

Authors
  1. Naeima Eftekhar
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  2. Ali Moghimi
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  3. Mohammad Hossein Boskabady
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Correspondence to Mohammad Hossein Boskabady.

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Eftekhar, N., Moghimi, A. & Boskabady, M.H. Prophylactic effect of rosmarinic acid on tracheal responsiveness, white blood cell count and oxidative stress markers in lung lavage of sensitized rats. Pharmacol. Rep 70, 119–125 (2018). https://doi.org/10.1016/j.pharep.2017.08.010

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  • DOI: https://doi.org/10.1016/j.pharep.2017.08.010

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