Abstract
Background
Chloroquine (CQ) evokes itch in human and scratching behavior in rodents through a histamine-independent pathway. Chloroquine directly excites peripheral sensory neurons which convey itch signals to the central nervous system. It has been revealed that ATP-sensitive potassium channels (KATP channels) are important in regulating neuronal excitability. Thus, we aimed to investigate the involvement of KATP channels in CQ-induced itch which may also reveal a linkage between metabolic state of cells and itch.
Methods
Intradermal (id) injection of CQ at dose of 400 μg/site induces the scratching behavior. KATP channel openers, diazoxide (DZX) and minoxidil (MIN), and a KATP channel blocker, glibenclamide (GLI), were administered intraperitoneally (ip) before CQ. Then the behavior was recorded for 30 min, in an unmanned condition, and the scratching bouts were counted by an expert observer who was blinded to the experiments. Furthermore, quantitative reverse transcription-PCR (qRT-PCR) was used to investigate the possible changes in dermal expression of Kcnj8 and Kcnj11, the genes encoding the KATP channels.
Results
Our results show that either DZX (10 mg/kg, ip) or MIN (10 mg/kg, ip) significantly attenuated CQ-induced scratching behavior in mice. Moreover, pretreatment with GLI (3 mg/kg, ip) significantly reversed the anti-pruritic effects of DZX and MIN. Our finding of qRT-PCR analysis also show that the expression of Kcnj8 is decreased after CQ injection.
Conclusions
We suggest that KATP channels are possibly involved in CQ-induced itch. While, further studies will be significant to better elucidate the association of metabolic state of cells and itch.
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Abbreviations
- ATP-sensitive potassium channels or KATP channels:
-
adenosine triphosphate sensitive potassium channels
- CQ:
-
chloroquine
- DZX:
-
diazoxide
- GLI:
-
glibenclamide
- id:
-
intradermal
- ip :
-
intraperitoneal
- MIN:
-
minoxidil
References
Akiyama T, Carstens E. Neural processing of itch. Neuroscience 2013;250:697–714.
Reich A, Ständer S, Szepietowski JC. Drug-induced pruritus: a review. Acta Derm Venereol 2009;89:236–44.
Mnyika K, Kihamia C. Chloroquine-induced pruritus: its impact on chloroquine utilization in malaria control in Dar es Salaam. J Trop Med Hyg 1991;94:27–31.
Liu Q, Tang Z, Surdenikova L, Kim S, Patel KN, Kim A, et al. Sensory neuron-specific GPCR Mrgprs are itch receptors mediating chloroquine-induced pruritus. Cell 2009;139:1353–65.
Green AD, Young KK, Lehto SG, Smith SB, Mogil JS. Influence of genotype, dose and sex on pruritogen-induced scratching behavior in the mouse. Pain 2006;124:50–8.
Haddadi N-S, Foroutan A, Ostadhadi S, Azimi E, Rahimi N, Nateghpour M, et al. Peripheral NMDA receptor/NO system blockage inhibits itch responses induced by chloroquine in mice. Acta Dem Venereol 2017;97.
Foroutan A, Haddadi NS, Ostadhadi S, Sistany N, Dehpour AR. Chloroquine-induced scratching is mediated by NO/cGMP pathway in mice. Pharmacol Biochem Behav 2015;134:79–84.
Wilson SR, Gerhold KA, Bifolck-Fisher A, Liu Q, Patel KN, Dong X, et al. TRPA1 is required for histamine-independent, mas-related G protein-coupled receptor-mediated itch. Nat Neurosci 2011;14:595–602.
Dascal N. Ion-channel regulation by G proteins. Trends Endocrinol Metab 2001;12:391–8.
Yamada K, Inagaki N. Neuroprotection by K ATP channels. J Mol Cell Cardiol 2005;38:945–9.
Ocaña M, Cendán CM, Cobos EJ, Entrena JM, Baeyens JM. Potassium channels and pain: present realities and future opportunities. Eur J Pharmacol 2004;500:203–19.
De Weille JR, Lazdunski M. Regulation of the ATP-sensitive potassium channel Ion channels. Springer; 1990. p. 205-22.
Takano N, Arai I, Kurachi M. Possible antipruritic effects of K+ channel openers in mice. J Dermatol Sci 2004;35:71–3.
Coavoy-Sánchez S, Rodrigues L, Teixeira S, Soares A, Torregrossa R, Wood M, et al. Hydrogen sulfide donors alleviate itch secondary to the activation of type-2 protease activated receptors (PAR-2) in mice. Pharmacol Res 2016;113:686–94.
Olfert ED, Cross BM, McWilliam AA. Guide to the care and use of experimental animals. Ottawa: Canadian Council on Animal Care; 1993.
Rajaba A, Ostadhadi S, Rastegar H, Dehpour A. Anti-pruritic activity of pioglitazone on serotonin-induced scratching in mice: possible involvement of PPAR-gamma receptor and nitric oxide. Eur J Pharmacol 2014;744:103–7.
Ostadhadi S, Haj-Mirzaian A, Azimi E, Mansouri P, Dehpour A. Involvement of nitric oxide in serotonin-induced scratching in mice. Clin Exp Dermatol 2015;40:647–52.
Kaster MP, Ferreira PK, Santos AR, Rodrigues AL. Effects of potassium channel inhibitors in the forced swimming test: possible involvement of L-argininenitric oxide-soluble guanylate cyclase pathway. Behav Brain Res 2005;165:204–9.
Ostadhadi S, Ahangari M, Nikoui V, Norouzi-Javidan A, Zolfaghari S, Jazaeri F, et al. Pharmacological evidence for the involvement of the NMDA receptor and nitric oxide pathway in the antidepressant-like effect of lamotrigine in the mouse forced swimming test. Biomed Pharmacother 2016;82:713–21.
Mohseni G, Ostadhadi S, Imran-Khan M, Norouzi-Javidan A, Zolfaghari S, Haddadi N-S, et al. Agmatine enhances the antidepressant-like effect of lithium in mouse forced swimming test through NMDA pathway. Biomed Pharmacother 2017;88:931–8.
Nikoui V, Mehr SE, Jazaeri F, Ostadhadi S, Eftekhari G, Dehpour A-R, et al. Prostaglandin F 2α modulates atrial chronotropic hyporesponsiveness to cholinergic stimulation in endotoxemic rats. Eur J Pharmacol 2015;748:149–56.
Momeny M, Ghasemi R, Valenti G, Miranda M, Zekri A, Zarrinrad G, et al. Effects of silibinin on growth and invasive properties of human ovarian carcinoma cells through suppression of heregulin/HER3 pathway. Tumor Biology 2016;37:3913–23.
Sun Y-G, Zhao Z-Q, Meng X-L, Yin J, Liu X-Y, Chen Z-F. Cellular basis of itch sensation. Science 2009;325:1531–4.
Cao D-S, Zhong L, Hsieh T-h, Abooj M, Bishnoi M, Hughes L, et al. Expression of transient receptor potential ankyrin 1 (TRPA1) and its role in insulin release from rat pancreatic beta cells. PLoS One 2012;7:e38005.
Schmidt-Nielsen K. Animal physiology: adaptation and environment. Cambridge University Press; 1997.
Baraniuk JN. Rise of the sensors: nociception and pruritus. Curr Allergy Asthma Rep 2012;12:104–14.
Sarantopoulos C, McCallum B, Sapunar D, Kwok W-M, Hogan Q. ATP-sensitive potassium channels in rat primary afferent neurons: the effect of neuropathic injury and gabapentin. Neurosci Lett 2003;343:185–9.
Freiman TM, Kukolja J, Heinemeyer J, Eckhardt K, Aranda H, Rominger A, et al. Modulation of K+-evoked [3H]-noradrenaline release from rat and human brain slices by gabapentin: involvement of KATP channels. Naunyn Schmiedebergs Arch Pharmacol 2001;363:537–42.
Schmid-Antomarchi H, Amoroso S, Fosset M, Lazdunski M. K+ channel openers activate brain sulfonylurea-sensitive K+ channels and block neurosecretion. Proc Natl Acad Sci U S A 1990;87:3489–92.
Yamashita S, Park JB, Ryu PD, Inukai H, Tanifuji M, Murase K. Possible presence of the ATP-sensitive K+ channel in isolated spinal dorsal horn neurons of the rat. Neurosci Lett 1994;170:208–12.
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Ostadhadi, S., Foroutan, A., Haddadi, NS. et al. Pharmacological evidence for the involvement of adenosine triphosphate sensitive potassium channels in chloroquine-induced itch in mice. Pharmacol. Rep 69, 1295–1299 (2017). https://doi.org/10.1016/j.pharep.2017.05.021
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DOI: https://doi.org/10.1016/j.pharep.2017.05.021