Abstract
The organic extracts from stems, roots and leaves of Tephrosia egregia Sandwith, Fabaceae, provided a new flavone, 5-hydroxy-8-(1″,2″-epoxy-3″-hydroxy-3″-methylbutyl)-7-methoxyflavone (1), in addition to eleven known compounds: pongaflavone (2), praecansone B (3), 12a-hydroxyrotenone (4), praecansone A, 2′,6′-dimethoxy-4′,5′-(2″,2″-dimethyl)-pyranochalcone, pongachalcone, maackiain, β-sistosterol and its glucoside, p-cumaric acid and cinnamic acid. The structures of all compounds were established on the basis of spectroscopic methods, mainly 1D and 2D NMR and HRESIMS, involving comparison with literature data. Cytotoxicity of compounds 1–4 was evaluated against AGP-01 (cancerous ascitic fluid), HCT-116 (colon adenocarcinoma), HL-60 (leukemia), PC-3 (prostate carcinoma), SF-295 (glioblastoma) and SKMEL 28 (melanoma) cell lines.
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MVST and JQL (MSc and PhD students, respectively), IGP, ACM, DCCF (undergraduate students) contributed running the laboratory work and drafted the paper; AMCA, MCFO, RBF, GMPS did the analysis and interpretation of data of RMN, critical revision of the manuscript; ATAP, MASL and FRLS carried out the separation on HPLC of pure compounds. PRVR acquisition and interpretation of Mass spectra. RCM, DDR, MMB and MEAM conducted and interpreted the cytotoxic assay. All the authors have read the final manuscript and approved the submission.
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Teixeira, M.V.S., Lima, J.Q., Pimenta, A.T.A. et al. New flavone and other compounds from Tephrosia egregia: assessing the cytotoxic effect on human tumor cell lines. Rev. Bras. Farmacogn. 28, 333–338 (2018). https://doi.org/10.1016/j.bjp.2018.03.008
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DOI: https://doi.org/10.1016/j.bjp.2018.03.008