Abstract
Moussonia deppeana (Schltdl. & Cham.) Klotzsch ex Hanst., Gesneriaceae, known as tlachichinole, is a Mexican medicinal plant used for treatment of chronic inflammation-related diseases such as arthritis. In this paper, the main metabolite verbascoside was quantified in ethanolic extract; anti-arthritic and antioxidant activities were also evaluated in Complete Freund’s Adjuvant induced arthritis in mice, with complete hematological evaluation, and oxidative stress measure in edema and ganglionic tissues on day 28. In popliteal ganglion, CD4+ lymphocytes and tumor necrosis factor alpha concentration were measured in addition to histological analysis. Ethanolic extract contained 79.2 mg of verbascoside/g extract, and this extract at 450 mg/kg generated an inhibition of 24% over paw edema development and increased body weight gain on final day. For hematological parameters, same dose decreased total leukocytes and lymphocytes, as well as decreased oxidation rate over biomolecules in edema and ganglionic tissues, and increased antioxidant enzyme activity. In ganglionic tissue, CD4+ lymphocytes and tumor necrosis factor alpha level showed no differences at any tested dose compared to complete Freund’s adjuvant untreated group. Histological analysis of popliteal ganglion revealed moderate reduction of follicular hyperplasia, leukocyte infiltration and lipid inclusions at 450 mg/kg dose. Ethanolic extract of M. deppeana possesses anti-edematous activity associated to a moderate reduction in follicular hyperplasia, with immune-modulatory and antioxidant effects during experimental arthritis in mice.
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GAGR contributed running all the laboratory work related to experimental arthritis establishment, taking tissues and blood samples, evaluation of antioxidant effect, interleukins concentration, extract preparation, statistical analysis and writing the final manuscript. LGS contributed to biological studies by giving all OS reagents and to facilitate the use of the spectrophotometer. AKCR contributed to biological studies by giving part of interleukins quantification reagents as well the laboratory for the establishment of these molecular biology assays, and for help in the interpretation of the results from flow cytometer. AGSR contributed with the histological analysis of sub-plantar edema and popliteal ganglion tissues, as well the interpretations after the stain. AZ contributed to chromatographic analysis and quantification of verbascoside fromEthanolic extract.
MAJA supervised the laboratory work and contributed to critical reading of the manuscript, besides obtaining the economic funds to be able to carry out the project.
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Gutiérrez-Rebolledo, G.A., Garduño-Siciliano, L., Chávez-Rueda, A.K. et al. In vivo anti-arthritic and antioxidant effects from the standardized ethanolic extract of Moussonia deppeana. Rev. Bras. Farmacogn. 28, 198–206 (2018). https://doi.org/10.1016/j.bjp.2018.02.004
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DOI: https://doi.org/10.1016/j.bjp.2018.02.004