Abstract
The phytochemical study of the extract leaves from Maytenus distichophylla Mart. and Salacia crassifolia (Mart. ex Schult.) G. Don, Celastraceae, resulted in the isolation of 3-oxofriedelane, 3β-hydroxyfriedelane, 3β,24-dihydroxyfriedelane, 3-oxo-28,29-dihydroxyfriedelane, two mixtures of pentacyclic triterpenes (α-amyrin with β-amyrin and 3β-stearyloxy-urs-12-ene with 3β-stearyloxy-olean-12-ene), 3β-palmityloxy-urs-12-ene, the steroid β-sitosterol and its glycosylated derivative β-glucosyl-β-sitosterol, tritriacontanoic acid and the natural polymer gutta percha. The chemical structures of these constituents were established by IR, 1H and 13C NMR spectral data. Crude extracts, the mixtures of triterpenes and the isolated constituents were subjected to in vitro acetylcholinesterase inhibitory evaluation. Acetylcholinesterase inhibitory effect was observed for crude chloroform extract leaves from M. distichophylla (100%) and S. crassifolia (97.93 ± 5.63%) and for the triterpenes 3β,24-dihydroxyfriedelane (99.05± 1.12%), 3-oxo-28,29-dihydroxyfriedelane (90.59±3.76%) and 3β-palmityloxy-urs-12-ene (97.93 ± 1.47%). The percent inhibitions induced by these natural products were very similar to those produced by physostigmine (93.94 ±2.10%) a standard acetylcholinesterase inhibitor. Therefore, these results open perspectives for the use of these species as source of compounds with similar physostigmine pharmacological effect.
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FLF, VGR, FCS, GDFS and LPD contributed to the experimental work, extraction and isolation of the metabolites from plants, the interpretation of all these data and wrote the manuscript. SAVF and DMO wrote and revised the manuscript. BLGM and JAT conducted the test in vitro AChE inhibitory activity. All authors read and approved the final manuscript.
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Ferreira, F.L., Rodrigues, V.G., Silva, F.C. et al. Maytenus distichophylla and Salacia crassifolia: source of products with potential acetylcholinesterase inhibition. Rev. Bras. Farmacogn. 27, 471–474 (2017). https://doi.org/10.1016/j.bjp.2016.12.006
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DOI: https://doi.org/10.1016/j.bjp.2016.12.006