Abstract
In the fast growing field of scaffold-based tissue engineering, improvement on the mechanical properties of newly formed tissues, e.g. the repaired cartilage, has always been one of the core issues. Studies on the correlations among scaffold composition, in vivo morphological changes of the construct, and the finite deformation behaviors of new tissues (e.g. creep and stress-relaxation, and equilibrium response), have attracted increasing interests. In this paper, the correlations between the compressive biphasic mechanical properties (i.e., equilibrium elastic modulus E and permeability coefficient k) of 3D printing scaffold (consisting of collagen and β-tricalcium phosphate) and the proteoglycans (PGs) concentration of the repaired cartilages after 24 weeks, 36 weeks and 52 weeks of scaffold implantation were investigated. Results indicated that the repaired cartilage covered the entire cartilage surface of large cylindrical osteochondral defects (10 mm in diameter × 15 mm in depth) on the canine trochlea grooves after 24 weeks. The equilibrium elastic modulus of the repaired cartilage reached 22.4% at 24 weeks, 70.3% at 36 weeks, and 93.4% at 52 weeks of the native cartilage, respectively. Meanwhile, the permeability coefficient decreased with time and at 52 weeks was still inferior to that of the native cartilage in one order of magnitude. In addition, the amount of glycosaminoglycans (GAGs) of repaired cartilage increased constantly with time, which at 52 weeks approached to nearly 60% of that of native cartilage. 3D printed scaffolds have potential applications in repairing large-scale cartilage defects.
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Pawaskar S S, Grosland N M, Ingham E, Fisher J, Jin Z. Hemiarthroplasty of hip joint: An experimental validation using porcine acetabulum. Journal of Biomechanics, 2011, 44, 1536–1542.
Keenan K E, Pal S, Lindsey D P, Besier T B. GS Simulating Creep Indentation Tests of Cartilage: Are Initial Indenter Interface Conditions the Cause of Poor Model Fits? Poster No. 2151, ORS Annual Meeting, 2011.
Thambyah A, Broom N. How subtle structural changes associated with maturity and mild degeneration influence the impact-induced failure modes of cartilage-on-bone. Clin Biomech, 2010, 25, 737–744.
Mow V C, Kuei S C, Lai W M, Armstrong C G. Biphasic creep and stress relaxation of articular cartilage in compression? Theory and experiments. Journal of Biomechanical Engineering, 1980, 102, 73–84.
Schaefer D, Martin I, Jundt G, Seidel J, Heberer M, Grodzinsky A, Bergin I, Vunjak-Novakovic G, Freed LE. Tissue-engineered composites for the repair of large osteochondral defects. Arthritis and Rheumatism, 2002, 46, 2524–2534.
Mow V C, Huiskes R. Basic Orthopaedic Biomechanics & Mechano-Biology, 3rd ed, Lippincott Williams & Wilkins 2005.
Katta J, Jin Z, Ingham E, Fisher J. Biotribology of articular cartilage-A review of the recent advances. Medical Engineering & Physics, 2008, 30, 1349–1363.
Laasanen M S, Toyras J, Korhonen R K, Rieppo J, Saarakkala S, Nieminen M T, Hirvonen J, Jurvelin J S. Biome chanical properties of knee articular cartilage. Biorheology, 2003, 40, 133–140.
Ateshian G A, Warden W H, Kim J J, Grelsamer R P, Mow V C. Finite deformation biphasic material properties of bovine articular cartilage from confined compression experiments. Journal of Biomechanics, 1997, 30, 1157–1164.
Pawaskar S S. Joint Contact Modelling of Articular Cartilage in Synovial Joints. Leeds, University of Leeds, 2010.
Jin Z, Pickard J E, Forster H, Ingham E, Fisher J. Frictional behaviour of bovine articular cartilage. Biorheology, 2000, 37, 57–63.
Meng Q, Jin Z, Wilcox R, Fisher J. Computational investigation of the time-dependent contact behaviour of the human tibiofemoral joint under body weight. Proceedings of the Institution of Mechanical Engineers Part H, Journal of Engineering in Medicine, 2014, 228, 1193–1207.
Mow V C, Ratcliffe A, Rosenwasser M P, Buckwalter J A. Experimental studies on repair of large osteochondral defects at a high weight bearing area of the knee joint: a tissue engineering study. Journal of Biomechanical Engineering, 1991, 113, 198–207.
Knecht S, Vanwanseele B, Stussi E. A review on the mechanical quality of articular cartilage-implications for the diagnosis of osteoarthritis. Clinical Biomechanics, 2006, 21, 999–1012.
Mak A F. The apparent viscoelastic behavior of articular cartilage-the contributions from the intrinsic matrix viscoelasticity and interstitial fluid flows. Journal of Biome- Chanical Engineering, 1986, 108, 123–130.
Temenoff J S, Mikos A G. Review: Tissue engineering for regeneration of articular cartilage. Biomaterials, 2000, 21, 431–440.
Panseri S, Russo A, Cunha C, Bondi A, Di Martino A, Patella S, Kon E. Osteochondral tissue engineering approaches for articular cartilage and subchondral bone regeneration. Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA, 2012, 20, 1182–1191.
Zhang L, Hu J, Athanasiou K A. The role of tissue engineering in articular cartilage repair and regeneration. Critical Reviews in Biomedical Engineering, 2009, 37, 1–57.
Athanasiou K, Korvick D, Schenck R. Biodegradable implants for the treatment of osteochondral defects in a goat model. Tissue Engineering, 1997, 3, 363–373.
Mayr H O, Klehm J, Schwan S, Hube R, Sudkamp N P, Niemeyer P, Salzmann G, von Eisenhardt-Rothe R, Heilmann A, Bohner M, Bernstein A. Microporous calcium phosphate ceramics as tissue engineering scaffolds for the repair of osteochondral defects: Biomechanical results. Acta biomaterialia, 2013, 9, 4845–4855.
Hu J C, Athanasiou K A. A self-assembling process in articular cartilage tissue engineering. Tissue Engineering, 2006, 12, 969–979.
Jansen E J, Pieper J, Gijbels M J, Guldemond N A, Riesle J, Van Rhijn L W, Bulstra S K, Kuijer R. PEOT/PBT based scaffolds with low mechanical properties improve cartilage repair tissue formation in osteochondral defects. Journal of Biomedical Materials Research Part A, 2009, 89, 444–452.
Zhang W, Lian Q, Li D, Wang K, Hao D, Bian W, He J, Jin Z. Cartilage repair and subchondral bone migration using 3D printing osteochondral composites: A one year period study in rabbit trochlea. Biomed Research International, 2014.
Zhang W, Lian Q, Li D, Wang K, Hao D, Bian W, Jin Z. The effect of interface microstructure on interfacial shear strength for osteochondral scaffolds based on biomimetic design and 3D printing. Materials Science & Engineering C, Materials for Biological Applications, 2015, 46, 10–15.
Bian W, Li D, Lian Q, Li X, Zhang W, Wang K, Jin Z. Fabrication of a bio-inspired beta-Tricalcium phosphate/ collagen scaffold based on ceramic stereolithography and gel casting for osteochondral tissue engineering. Rapid Prototyping Journal, 2012, 18, 68–80.
Pawaskar S S, Fisher J, Jin Z. Robust and general method for determining surface fluid flow boundary conditions in articular cartilage contact mechanics modeling. Journal of Biomechanical Engineering, 2010, 132, 031001.
Martin I, Miot S, Barbero A, Jakob M, Wendt D. Osteochondral tissue engineering. Journal of Biomechanics, 2007, 40, 750–765.
Soltz M A, Ateshian G A. Experimental verification and theoretical prediction of cartilage interstitial fluid pressurization at an impermeable contact interface in confined compression. Journal of Biomechanics, 1998, 31, 927–934.
Keenan K E, Kourtis L C, Besier T F, Lindsey D P, Gold G E, Delp S L, Beaupre G S. New resource for the computation of cartilage biphasic material properties with the interpolant response surface method. Computer Methods in Biomechanics and Biomedical Engineering, 2009, 12, 415–422.
Roemhildt M L, Coughlin K M, Peura G D, Fleming B C, Beynnon B D. Material properties of articular cartilage in the rabbit tibial plateau. Journal of Biomechanics, 2006, 39, 2331–2337.
Mow V C, Gibbs M C, Lai W M, Zhu W B, Athanasiou K A. Biphasic indentation of articular-cartilage. 2. A numerical algorithm and an experimental-study. Journal of Biomechanics, 1989, 22, 853–861.
Scotti C, Wirz D, Wolf F, Schaefer D J, Burgin V, Daniels A U, Valderrabano V, Candrian C, Jakob M, Martin I, Barbero A. Engineering human cell-based, functionally integrated osteochondral grafts by biological bonding of engineered cartilage tissues to bony scaffolds. Biomaterials, 2010, 31, 2252–2259.
Korhonen R K, Laasanen M S, Toyras J, Rieppo J, Hirvonen J, Helminen H J, Jurvelin J S. Comparison of the equilibrium response of articular cartilage in unconfined compression, confined compression and indentation. Journal of Biomechanics, 2002, 35, 903–909.
Athanasiou K A, Rosenwasser M P, Buckwalter J A, Malinin T I, Mow V C. Interspecies comparisons of in situ intrinsic mechanical properties of distal femoral cartilage. Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society, 1991, 9, 330–340.
Tuli R, Nandi S, Li W J, Tuli S, Huang X, Manner P A, Laquerriere P, Noth U, Hall D J, Tuan R S. Human mesenchymal progenitor cell-based tissue engineering of a single- unit osteochondral construct. Tissue Engineering, 2004, 10, 1169–1179.
Grayson W L, Chao P-HG, Marolt D, Kaplan D L, Vunjak- Novakovic G. Engineering custom-designed osteochondral tissue grafts. Trends in Biotechnology, 2008, 26, 181–189.
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Lian, Q., Chen, C., Uwayezu, M.C. et al. Biphasic mechanical properties of in vivo repaired cartilage. J Bionic Eng 12, 473–482 (2015). https://doi.org/10.1016/S1672-6529(14)60138-4
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DOI: https://doi.org/10.1016/S1672-6529(14)60138-4