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Quantitative analysis of methanol in blood, urine, vitreous humor, and cerebrospinal fluid by using gas chromatography-head space in two suspected cases of methanol poisoning

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Abstract

Methanol is a toxic alcohol and can be encountered in clinical and forensic cases. Methanol toxicity is primarily related to its metabolic products-formaldehyde and formic acid, which can cause metabolic acidosis, blindness, CNS disorders, coma, or even death. Quantitative determination and elucidation of methanol levels is crucial in clinical and forensic toxicology. Gas chromatography-headspace (GC-HS) can be used to determine methanol concentration in various biological fluids. This study reports two cases of suspected methanol poisoning. Blood, urine, vitreous humor, and cerebrospinal fluid (CSF) samples were taken for toxicological analysis during post-mortem examination. The samples were run in GC-HS for analysis of ethanol and methanol. The analysis only required 1 ml of sample and the addition of internal standard (IS). Total runtime of analysis was achieved within 20 min. Methanol was successfully detected in blood, urine, vitreous humor, and CSF using GC-HS. GC-HS is efficient for the determination of methanol concentrations, owing to its high specificity, reliability sensitivity, and reproducibility. This study also emphasizes the collection and analysis of multiple specimens, including those that are more resistant to post-mortem changes, so that they can be tested in cases where a blood sample is not readily obtainable due to alterations, putrefaction, etc. The use of an alternative specimen to the blood is of great relevance for forensic purposes.

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References

  • Ashurst, J.V., Nappe, T.M.: Methanol toxicity (2018)

  • Ashurst, J.V., Nappe, T.M.: Methanol toxicity. StatPearls (Internet) (2021 Jan.)

  • Bodwal, J., Chauhan, M.S., Ghosh, M., Behera, C.: Hooch tragedies in India: a review. Anil Aggrawal’s Int. J. Forensic Med. Toxicol. 15(2), 1 (2014)

    Google Scholar 

  • Bonchev, G.D., Zlateva, S.Z., Yovcheva, M.I.: Toxicological chemical analysis of methanol in blood of patients with acute ethanol intoxication for determining detectable quantities of methanol and analysis of the correlation between ingested alcohol. J. Biomed. Clin. Res. 9(1), 48–54 (2016)

    Article  Google Scholar 

  • Caplan, Y.H., Levine, B.: Vitreous humor in the evaluation of postmortem blood ethanol concentrations. J. Anal. Toxicol. 14(5), 305–307 (1990)

    Article  CAS  Google Scholar 

  • Ceelen, M., Dorn, T., Buster, M., Stomp, J., Zweipfenning, P., Das, K.: Post-mortem toxicological urine screening in cause of death determination. Hum. Exp. Toxicol. 30(9), 1165–1173 (2011)

    Article  CAS  Google Scholar 

  • Challenges and Trends in GC Headspace Analysis (Internet): Lab manager (2022). Available from: https://www.labmanager.com/ask-the-expert/challenges-and-trends-in-gc-headspace-analysis-2294

  • D’Silva, J.: India’s problem with toxic alcohol. BMJ 25, 351 (2015)

    Google Scholar 

  • Desai, T., Sudhalkar, A., Vyas, U., Khamar, B.: Methanol poisoning: predictors of visual outcomes. JAMA Ophthalmol. 131(3), 358–364 (2013)

    Article  CAS  Google Scholar 

  • Druid, H., Holmgren, P.: A compilation of fatal and control concentrations of drugs in postmortem femoral blood. J. Forensic Sci. 42(1), 79–87 (1997)

    Article  Google Scholar 

  • Edenberg, H.J.: The genetics of alcohol metabolism: role of alcohol dehydrogenase and aldehyde dehydrogenase variants. Alcohol. Res. Health. 30(1), 1–5 (2007)

    Google Scholar 

  • Haffner, H.T., Wehner, H.D., Scheytt, K.D., Besserer, K.: The elimination kinetics of methanol and the influence of ethanol. Int. J. Legal Med. 105, 111–114 (1992)

    Article  CAS  Google Scholar 

  • Ioan, B.G., Jitaru, V., Damian, R., Damian, S.I.: Study on the relationship between the concentration of ethanol in the blood, urine and the vitreous humour. Roman. J. Leg. Med. 23(3), 211–216 (2015)

    Article  Google Scholar 

  • Islek, D.S., Ramadanoglu, S.: Headspace-gas chromatography/mass spectrometry analysis of methanol in blood. Medicine 6(2), 372–374 (2017)

    Google Scholar 

  • Jollymore, B.D., Fraser, A.D., Moss, M.A., Perry, R.A.: Comparative study of ethyl alcohol in blood and vitreous humor. Can. Soc. Forensic Sci. J. 17(2), 50–54 (1984)

    Article  CAS  Google Scholar 

  • Jones, A.W.: Clinical and forensic toxicology of methanol. Forensic Sci. Rev. 33(2), 117–143 (2021)

    CAS  Google Scholar 

  • Lee, X.P., Kumazawa, T., Kondo, K., Sato, K., Suzuki, O.: Analysis of methanol or formic acid in body fluids by headspace solid-phase microextraction and capillary gas chromatography. J. Chromatogr. B Biomed. Sci. Appl. 734(1), 155–162 (1999)

    Article  CAS  Google Scholar 

  • Lee, J.B., Jeong, Y.A., Ahn, D.J., Bang, I.S.: SPME-GC/MS analysis of methanol in biospecimen by derivatization with pyran compound. Molecules 25(1), 41 (2019)

    Article  Google Scholar 

  • Maleki, R., Farhadi, K., Matin, A.A.: Analysis of ethanol and methanol in human body fluids by headspace solid phase microextraction coupled with capillary gas chromatography. Anal. Sci. 22(9), 1253–1255 (2006)

    Article  CAS  Google Scholar 

  • Meyer, R.J., Beard, M.E., Ardagh, M.W., Henderson, S.: Methanol poisoning. N. z. Med. J. 113(1102), 11 (2000)

    CAS  Google Scholar 

  • Mihretu, L.D., Gebru, A.G., Mekonnen, K.N., Asgedom, A.G., Desta, Y.H.: Determination of ethanol in blood using headspace gas chromatography with flame ionization detector (HS-GC-FID): validation of a method. Cogent Chem. 6(1), 1760187 (2020)

    Article  Google Scholar 

  • Na, J.Y., Park, J.H., Oh, Y.H., Oh, S.M., Kim, H.S., Park, J.T.: Analysis of cisternal puncture results during a postmortem examination. Korean J. Leg. Med. 40(2), 39–43 (2016)

    Article  Google Scholar 

  • Neil, P., Mills, A.J., Prabhakaran, V.M.: Evaluation of vitreous humor and urine alcohol levels as indices of blood alcohol levels in 75 autopsy cases. Can. Soc. Forensic Sci. J. 18(2), 97–104 (1985)

    Article  CAS  Google Scholar 

  • Nekoukar, Z., Zakariaei, Z., Taghizadeh, F., Musavi, F., Banimostafavi, E.S., Sharifpour, A., Ghuchi, N.E., Fakhar, M., Tabaripour, R., Safanavaei, S.: Methanol poisoning as a new world challenge: a review. Ann. Med. Surg. 1(66), 102445 (2021)

    Google Scholar 

  • Ott J, Gronemann V, Pontzen F, Fiedler E, Grossmann G, Kersebohm DB, Weiss G,Witte C. Methanol. Ullmann's encyclopedia of industrial chemistry. 2 (2000)

  • Pressman, P., Clemens, R., Sahu, S., Hayes, A.W.: A review of methanol poisoning: a crisis beyond ocular toxicology. Cutan. Ocul. Toxicol. 39(3), 173–179 (2020)

    Article  CAS  Google Scholar 

  • Rahimi, R., Zainun, K.A., Noor, N.M., Kasim, N.A., Shahrir, N.F., Azman, N.A., Elankopan, R., Nikman, K.A., Room, N.H.: Methanol poisoning in Klang Valley, Malaysia: autopsy case series. Forensic Sci. Int. Rep. 1(3), 100170 (2021)

    Article  Google Scholar 

  • Salendu, A.R., Meilia, P.D.: Clinical and pathological findings in multiple deaths due to dissolved alcohol toxicity (2017)

  • Seth, S.: Review of Forensic Medicine, pp. 266–267. Peepee Publishers & Distribution, New York (2018)

    Google Scholar 

  • Skopp, G.: Postmortem toxicology. Forensic Sci. Med. Pathol. 6, 314–325 (2010)

    Article  CAS  Google Scholar 

  • Tephly, T.R.: The toxicity of methanol. Life Sci. 48(11), 1031–1041 (1991)

    Article  CAS  Google Scholar 

  • Tian, M., He, H., Liu, Y., Li, R., Zhu, B., Cao, Z.: Fatal methanol poisoning with different clinical and autopsy findings: case report and literature review. Leg. Med. 1(54), 101995 (2022)

    Article  Google Scholar 

  • Tumram, N.K., Bardale, R.V., Dongre, A.P.: Postmortem analysis of synovial fluid and vitreous humour for determination of death interval: a comparative study. Forensic Sci. Int. 204(1–3), 186–190 (2011)

    Article  CAS  Google Scholar 

  • Weinberg, L., Stewart, J., Wyatt, J.P., Mathew, J.: Unexplained drowsiness and progressive visual loss: methanol poisoning diagnosed at autopsy. Emerg. Med. 15(1), 97–99 (2003)

    Article  Google Scholar 

  • Williams, G.F., Hatch, F.J., Bradley, M.C.: Methanol poisoning: a review and case study of four patients from central Australia. Aust. Crit. Care 10(4), 113–118 (1997)

    Article  CAS  Google Scholar 

  • Wolthers, H.: Undersøgelser over Postmortal Alkoholdannelse, pp. 1–79. Christtreus Bogtrykkeri, Copenhagen (1958)

    Google Scholar 

Download references

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, New Delhi, 110029, India

    Neha Afaria, A. K. Jaiswal, Venkatesh Janarthanan, Sudhir Kumar Gupta, T. Millo & G. Gokul

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  1. Neha Afaria
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  2. A. K. Jaiswal
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  3. Venkatesh Janarthanan
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  4. Sudhir Kumar Gupta
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  5. T. Millo
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  6. G. Gokul
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Correspondence to A. K. Jaiswal.

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Afaria, N., Jaiswal, A.K., Janarthanan, V. et al. Quantitative analysis of methanol in blood, urine, vitreous humor, and cerebrospinal fluid by using gas chromatography-head space in two suspected cases of methanol poisoning. Proc.Indian Natl. Sci. Acad. 89, 731–739 (2023). https://doi.org/10.1007/s43538-023-00179-5

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