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1H NMR Quantification of Polydatin and Emodin in Huganning, a Chinese Patent Herbal Medicine

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Abstract

Huganning, a Chinese patent medicine, has been used in clinical practice for the treatment of hepatitis in China for more than 20 years, and its main physiologically active components are polydatin and emodin. In this study, a rapid and accurate proton nuclear magnetic resonance method for the quantification of these constituents was developed. 4-Nitrochlorobenzene was selected as an internal standard, and the signals for polydatin at δ 6.44 ppm and emodin at δ 7.54 ppm were chosen for the quantification. Validation of the method was performed in terms of specificity, precision, and stability. The results indicated that the method was precise and in line with the China Food and Drug Administration guidance. The method has been successfully applied to characterize tablets and capsules obtained from six pharmaceutical companies. This study indicated that the rapid 1H NMR method is more suitable for the quality control of Huganning than the high-performance liquid chromatography method.

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Funding

This work was supported by the Open Research Fund Program of Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology (Ocean University of China), Fundamental Research Funds for the Central Universities (No. 201912002), and Shandong Provincial Natural Science Foundation, China (No. ZR2019BC025).

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Author notes

  1. Cong Wang and Pengpeng Zhang contributed equally to this work.

Authors and Affiliations

  1. School of Medicine and Pharmacy, Ocean University of China, Qingdao, People’s Republic of China

    Cong Wang, Pengpeng Zhang, Xiuli Zhang, Mingming Yu & Zhihua Lv

  2. Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao, People’s Republic of China

    Cong Wang, Xiuli Zhang, Mingming Yu & Zhihua Lv

  3. Key Laboratory of Glycoscience and Glycotechnology of Shandong Province, Qingdao, People’s Republic of China

    Cong Wang, Xiuli Zhang, Mingming Yu & Zhihua Lv

  4. Key Laboratory of Marine Drugs, Ministry of Education of China, Qingdao, People’s Republic of China

    Cong Wang, Xiuli Zhang, Mingming Yu & Zhihua Lv

Authors
  1. Cong Wang
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  2. Pengpeng Zhang
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  3. Xiuli Zhang
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  4. Mingming Yu
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  5. Zhihua Lv
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Contributions

WC and ZXL supervised the NMR analysis. ZPP extracted the samples and carried out the HPLC analysis. YMM and WC wrote the manuscript. YMM and LZH created the project and supervised the laboratory work. All of the authors have read the final manuscript and approved the submission.

Corresponding authors

Correspondence to Mingming Yu or Zhihua Lv.

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The authors declare that they have no conflict of interest.

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The authors declare that no experiments were performed on humans or animals for this study.

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The authors declare that no patient data appear in this article.

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Wang, C., Zhang, P., Zhang, X. et al. 1H NMR Quantification of Polydatin and Emodin in Huganning, a Chinese Patent Herbal Medicine. Rev. Bras. Farmacogn. 30, 28–33 (2020). https://doi.org/10.1007/s43450-020-00031-7

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  • DOI: https://doi.org/10.1007/s43450-020-00031-7

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