Abstract
A quantitative 1H-NMR method (qHNMR) was used to measure paeoniflorin content in Paeoniae radix (dried root of Paeonia lactiflora), of which paeoniflorin is a major component. The purity of paeoniflorin was calculated from the ratio of the intensity of the H-9 signal at δ 5.78 ppm of paeoniflorin to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using bisphenol A of certified reference material (CRM) grade. The paeoniflorin content in 2 separate samples of Paeoniae radix was determined by qHNMR and was found to be 2.15 and 2.45 %. We demonstrated that this method is useful for quantitative analysis of crude drugs.
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Acknowledgments
We thank Dr. N. Sugimoto of the National Institute of Health Sciences for kind advice on measurement using qHNMR.
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Tanaka, R., Yamazaki, M., Hasada, K. et al. Application of quantitative 1H-NMR method to determination of paeoniflorin in Paeoniae radix. J Nat Med 67, 657–661 (2013). https://doi.org/10.1007/s11418-012-0711-6
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DOI: https://doi.org/10.1007/s11418-012-0711-6