Abstract
This study evaluated the effect of temperature in the thermophilic range (45, 55, and 65 °C) for simultaneous production of hydrogen and ethanol in an anaerobic fluidized bed reactor (AFBR) using cassava wastewater as substrate, with a hydraulic retention time of 2 h. Substrate concentration varied from 4.5 to 5.5 g COD L−1. Maximum yields of hydrogen (1.98 mol-H2 mol−1-glucose) and ethanol (2.06 mol-EtOH mol−1-glucose) were achieved at 55 °C. In this condition, the hydrogen production rate was 2.05 L h−1 L−1, increased by 86% regarding 45 ºC. The concentration of organic acids reduced about 42% with increasing temperature from 45 to 55 °C and diminished 37% from 55 to 65 °C. Acetic acid prevailed over other metabolites in percentages about 41–51%, followed by butyrate at 45 ºC (33.8%), and ethanol at 55 ºC (25.5%) and 65 ºC (30.8%). The ethanol concentration remained practically constant (4.00 ± 1.51–4.74 ± 1.44 mM) if considering the associated uncertainty range. Overall, thermophilic temperatures were favorable for biofuel production, however, it is suggested to study other HRT in future work to avoid overloading the system.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amorim NCS, Alves I, Martins JS, Amorim ELC (2014) Biohydrogen production from cassava wastewater in an anaerobic fluidized bed reactor. Braz J Chem Eng 31:603–612. https://doi.org/10.1590/0104-6632.20140313s00002458
Amorim NCS, Amorim ELC, Kato MT et al (2018) The effect of methanogenesis inhibition, inoculum and substrate concentration on hydrogen and carboxylic acids production from cassava wastewater. Biodegradation 29:41–58. https://doi.org/10.1007/s10532-017-9812-y
Andreani CL, Tonello TU, Mari AG et al (2019) Impact of operational conditions on development of the hydrogen-producing microbial consortium in an AnSBBR from cassava wastewater rich in lactic acid. Int J Hydrog Energy 44:1474–1482. https://doi.org/10.1016/J.IJHYDENE.2018.11.155
Antonopoulou G, Gavala HN, Skiadas IV, Lyberatos G (2010) Influence of pH on fermentative hydrogen production from sweet sorghum extract. Int J Hydrog Energy 35:1921–1928. https://doi.org/10.1016/j.ijhydene.2009.12.175
APHA (2017) Standard methods for examination of water and wastewater, 23rd edn. American Public Health Association, Washington
Barros AR, Silva EL (2012) Hydrogen and ethanol production in anaerobic fluidized bed reactors: performance evaluation for three support materials under different operating conditions. Biochem Eng J 61:59–65. https://doi.org/10.1016/j.bej.2011.12.002
Buhr HO, Andrews JF (1977) Review paper: the thermophilic anaerobic digestion process. Water Res 11:129–143. https://doi.org/10.1016/0043-1354(77)90118-X
Cappelletti BM, Reginatto V, Amante ER, Antônio RV (2011) Fermentative production of hydrogen from cassava processing wastewater by Clostridium acetobutylicum. Renew Energy 36:3367–3372. https://doi.org/10.1016/j.renene.2011.05.015
Chavadej S, Wangmor T, Maitriwong K et al (2019) Separate production of hydrogen and methane from cassava wastewater with added cassava residue under a thermophilic temperature in relation to digestibility. J Biotechnol 291:61–71. https://doi.org/10.1016/J.JBIOTEC.2018.11.015
Chaves TC, Gois GNSB, Peiter FS et al (2021) Biohydrogen production in an AFBR using sugarcane molasses. Bioprocess Biosyst Eng 44:307–316. https://doi.org/10.1007/s00449-020-02443-0
Chen Y, Zhang X, Chen Y (2021) Propionic acid-rich fermentation (PARF) production from organic wastes: a review. Bioresour Technol 339:125569. https://doi.org/10.1016/J.BIORTECH.2021.125569
Cremonez PA, Teleken JG, Weiser Meier TR, Alves HJ (2021) Two-Stage anaerobic digestion in agroindustrial waste treatment: a review. J Environ Manag 281:111854. https://doi.org/10.1016/j.jenvman.2020.111854
Cruz IA, Santos Andrade LR, Bharagava RN et al (2021) Valorization of cassava residues for biogas production in Brazil based on the circular economy: An updated and comprehensive review. Clean Eng Technol 4:100196. https://doi.org/10.1016/j.clet.2021.100196
da Silva AN, Macêdo WV, Sakamoto IK et al (2019) Biohydrogen production from dairy industry wastewater in an anaerobic fluidized-bed reactor. Biomass Bioenerg 120:257–264. https://doi.org/10.1016/j.biombioe.2018.11.025
Dahiya S, Chatterjee S, Sarkar O, Mohan SV (2021) Renewable hydrogen production by dark-fermentation: current status, challenges and perspectives. Bioresour Technol 321:124354. https://doi.org/10.1016/J.BIORTECH.2020.124354
Damasceno S, Cereda MP, Pastore GM, Oliveira JG (2003) Production of volatile compounds by Geotrichum fragrans using cassava wastewater as substrate. Process Biochem 39:411–414. https://doi.org/10.1016/S0032-9592(03)00097-9
de Amorim ELCA, Barros AR, RissatoZamariolliDamianovic MH, Silva EL (2009) Anaerobic fluidized bed reactor with expanded clay as support for hydrogen production through dark fermentation of glucose. Int J Hydrog Energy 34:783–790. https://doi.org/10.1016/j.ijhydene.2008.11.007
de Amorim ELC, Sader LT, Silva EL et al (2012) Effect of substrate concentration on dark fermentation hydrogen production using an anaerobic fluidized bed reactor. Appl Biochem Biotechnol 166:1248–1263. https://doi.org/10.1007/s12010-011-9511-9
de Carvalho JC, Borghetti IA, Cartas LC et al (2018) Biorefinery integration of microalgae production into cassava processing industry: potential and perspectives. Bioresour Technol 247:1165–1172. https://doi.org/10.1016/j.biortech.2017.09.213
Detman A, Laubitz D, Chojnacka A et al (2021) Dynamics of dark fermentation microbial communities in the light of lactate and butyrate production. Microbiome 9:158. https://doi.org/10.1186/s40168-021-01105-x
Díaz I, Fdz-Polanco F, Mutsvene B, Fdz-Polanco M (2020) Effect of operating pressure on direct biomethane production from carbon dioxide and exogenous hydrogen in the anaerobic digestion of sewage sludge. Appl Energy 280:115915. https://doi.org/10.1016/J.APENERGY.2020.115915
DuBois M, Gilles KA, Hamilton JK et al (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356. https://doi.org/10.1021/ac60111a017
Elbeshbishy E, Dhar BR, Nakhla G, Lee H-S (2017) A critical review on inhibition of dark biohydrogen fermentation. Renew Sustain Energy Rev 79:656–668. https://doi.org/10.1016/j.rser.2017.05.075
Ferraz FM, Bruni AT, Del Bianchi VL (2009) Performance of an Anaerobic Baffled Reactor (ABR) in treatment of cassava wastewater. Braz J Microbiol 40:48–53. https://doi.org/10.1590/S1517-83822009000100007
Ferreira TB, Rego GC, Ramos LR et al (2019) HRT control as a strategy to enhance continuous hydrogen production from sugarcane juice under mesophilic and thermophilic conditions in AFBRs. Int J Hydrog Energy 44:19719–19729. https://doi.org/10.1016/j.ijhydene.2019.06.050
Fioretto RA (2001) Uso direto da manipueira em fertirrigação. Manejo, uso e tratamento de subprodutos da industrialização da mandioca, v. 4. Fundação Cargill, São Paulo
Fleck L, Tavares MHF, Eyng E et al (2017) Optimization of anaerobic treatment of cassava processing wastewater. Eng Agrícola 37:574–590. https://doi.org/10.1590/1809-4430-eng.agric.v37n3p574-590/2017
Fu SF, Liu R, Sun WX et al (2020) Enhancing energy recovery from corn straw via two-stage anaerobic digestion with stepwise microaerobic hydrogen fermentation and methanogenesis. J Clean Prod 247:119651. https://doi.org/10.1016/J.JCLEPRO.2019.119651
Fuess LT, dos Santos GM, Delforno TP et al (2020) Biochemical butyrate production via dark fermentation as an energetically efficient alternative management approach for vinasse in sugarcane biorefineries. Renew Energy 158:3–12. https://doi.org/10.1016/J.RENENE.2020.05.063
Garcia-Aguirre J, Aymerich E, González-Mtnez J, de Goñi J, Esteban-Gutiérrez M (2017) Selective VFA production potential from organic waste streams: assessing temperature and pH influence. Bioresour Technol 244:1081–1088. https://doi.org/10.1016/J.BIORTECH.2017.07.187
García-Depraect O, Castro-Muñoz R, Muñoz R et al (2021) A review on the factors influencing biohydrogen production from lactate: the key to unlocking enhanced dark fermentative processes. Bioresour Technol 324:124595. https://doi.org/10.1016/J.BIORTECH.2020.124595
Gaurav N, Sivasankari S, Kiran GS et al (2017) Utilization of bioresources for sustainable biofuels: a review. Renew Sustain Energy Rev 73:205–214. https://doi.org/10.1016/j.rser.2017.01.070
Ge H, Jensen PD, Batstone DJ (2011) Relative kinetics of anaerobic digestion under thermophilic and mesophilic conditions. Water Sci Technol 64:848–853. https://doi.org/10.2166/wst.2011.571
Ghimire A, Frunzo L, Pirozzi F et al (2015) A review on dark fermentative biohydrogen production from organic biomass: process parameters and use of by-products. Appl Energy 144:73–95. https://doi.org/10.1016/j.apenergy.2015.01.045
Gois GNSB, Macêdo WV, Peiter FS et al (2021) Evaluation of biohydrogen production from sugarcane vinasse in an anaerobic fluidized bed reactor without pH control. Lat Am Appl Res 51:63–69. https://doi.org/10.52292/J.LAAR.2021.504
Gupta M, Gomez-Flores M, Nasr N et al (2015) Performance of mesophilic biohydrogen-producing cultures at thermophilic conditions. Bioresour Technol 192:741–747. https://doi.org/10.1016/j.biortech.2015.06.047
Hallenbeck PC, Benemann JR (2002) Biological hydrogen production; fundamentals and limiting processes. Int J Hydrog Energy 27:1185–1193. https://doi.org/10.1016/S0360-3199(02)00131-3
He M, Sun Y, Zou D et al (2012) Influence of temperature on hydrolysis acidification of food waste. Proced Environ Sci 16:85–94. https://doi.org/10.1016/j.proenv.2012.10.012
Hwang MH, Jang NJ, Hyun SH, Kim IS (2004) Anaerobic bio-hydrogen production from ethanol fermentation: the role of pH. J Biotechnol 111:297–309. https://doi.org/10.1016/j.jbiotec.2004.04.024
Intanoo P, Rangsanvigit P, Malakul P, Chavadej S (2014) Optimization of separate hydrogen and methane production from cassava wastewater using two-stage upflow anaerobic sludge blanket reactor (UASB) system under thermophilic operation. Bioresour Technol 173:256–265. https://doi.org/10.1016/J.BIORTECH.2014.09.039
Jiang J, Zhang Y, Li K et al (2013) Bioresource Technology Volatile fatty acids production from food waste : effects of pH, temperature, and organic loading rate. Bioresour Technol 143:525–530. https://doi.org/10.1016/j.biortech.2013.06.025
Jiang M, Wu Z, Yao J et al (2021) Enhancing the performance of thermophilic anaerobic digestion of food waste by introducing a hybrid anaerobic membrane bioreactor. Bioresour Technol 341:125861. https://doi.org/10.1016/J.BIORTECH.2021.125861
Karakashev D, Angelidaki I (2011) Thermophilic biohydrogen production. Biofuels. https://doi.org/10.1016/B978-0-12-385099-7.00024-3
Kerčmar J, Pintar A (2017) Support material dictates the attached biomass characteristics during the immobilization process in anaerobic continuous-flow packed-bed bioreactor. Anaerobe 48:194–202. https://doi.org/10.1016/j.anaerobe.2017.06.007
Khongkliang P, Kongjan P, O-Thong S (2015) Hydrogen and methane production from starch processing wastewater by thermophilic two-stage anaerobic digestion. Energy Proced 79:827–832. https://doi.org/10.1016/J.EGYPRO.2015.11.573
Khongkliang P, Kongjan P, Utarapichat B et al (2017) Continuous hydrogen production from cassava starch processing wastewater by two-stage thermophilic dark fermentation and microbial electrolysis. Int J Hydrog Energy 42:27584–27592. https://doi.org/10.1016/J.IJHYDENE.2017.06.145
Kim M, Ahn YH, Speece RE (2002) Comparative process stability and efficiency of anaerobic digestion; mesophilic vs. thermophilic. Water Res 36:4369–4385. https://doi.org/10.1016/S0043-1354(02)00147-1
Kim M, Gomec CY, Ahn Y, Speece RE (2003) Hydrolysis and acidogenesis of particulate organic material in mesophilic and thermophilic anaerobic digestion. Environ Technol 24:1183–1190. https://doi.org/10.1080/09593330309385659
Koskinen PEP, Lay CH, Puhakka JA et al (2008) High-efficiency hydrogen production by an anaerobic, thermophilic enrichment culture from an icelandic hot spring. Biotechnol Bioeng 101:665–678. https://doi.org/10.1002/bit.21948
Lay J, Fan K, Chang J, Ku C (2003) In uence of chemical nature of organic wastes on their conversion to hydrogen by heat-shock digested sludge. Int J Hydrog Energy 28:1361–1367. https://doi.org/10.1016/S0360-3199(03)00027-2
Levin DB, Pitt L, Love M (2004) Biohydrogen production: Prospects and limitations to practical application. Int J Hydrog Energy 29:173–185. https://doi.org/10.1016/S0360-3199(03)00094-6
Li J, Li B, Zhu G et al (2007) Hydrogen production from diluted molasses by anaerobic hydrogen producing bacteria in an anaerobic baffled reactor (ABR). Int J Hydrog Energy 32:3274–3283. https://doi.org/10.1016/j.ijhydene.2007.04.023
Liu C, Wang W, Anwar N et al (2017) Effect of organic loading rate on anaerobic digestion of food waste under mesophilic and thermophilic conditions. Energy Fuels 31:2976–2984. https://doi.org/10.1021/acs.energyfuels.7b00018
Liu Y, Liang T, Yuan X, Lv Y (2019) The performance of COD removal and hydrogen production in a single stage system from starch using the consortium PB-Z under simulated natural conditions. Energy 173:951–958. https://doi.org/10.1016/j.energy.2019.02.142
Lopes HJS, Ramos LR, de Menezes CA, Silva EL (2020) Simultaneous hydrogen and ethanol production in a thermophilic AFBR: a comparative approach between cellulosic hydrolysate single fermentation and the fermentation of glucose and xylose as co-substrates. Cellulose 27:2599–2612. https://doi.org/10.1007/s10570-020-03000-4
Lopez-Hidalgo AM, Magaña G, Rodriguez F et al (2021) Co-production of ethanol-hydrogen by genetically engineered Escherichia coli in sustainable biorefineries for lignocellulosic ethanol production. Chem Eng J 406:126829. https://doi.org/10.1016/j.cej.2020.126829
Luo G, Xie L, Zou Z et al (2010) Fermentative hydrogen production from cassava stillage by mixed anaerobic microflora: effects of temperature and pH. Appl Energy 87:3710–3717. https://doi.org/10.1016/j.apenergy.2010.07.004
Luo J, Feng L, Chen Y et al (2015) Alkyl polyglucose enhancing propionic acid enriched short-chain fatty acids production during anaerobic treatment of waste activated sludge and mechanisms. Water Res 73:332–341. https://doi.org/10.1016/J.WATRES.2015.01.041
Maintinguer SI, Fernandes BS, Duarte ICSS et al (2008) Fermentative hydrogen production by microbial consortium. Int J Hydrog Energy 33:4309–4317. https://doi.org/10.1016/j.ijhydene.2008.06.053
Mota VT, Santos FS, Araújo TA, Amaral MCS (2018) Evaluation of titration methods for volatile fatty acids measurement: effect of the bicarbonate interference and feasibility for the monitoring of anaerobic reactors. Water Pract Technol. https://doi.org/10.2166/wpt.2015.056
Nan-qi R, Zhao D, Chen X, Li J (2002) Mechanism and controlling strategy of the production and accumulation of propionic acid for anaerobic wastewater treatment. Sci China-Chem 45:319–327
Nie E, He P, Zhang H et al (2021) How does temperature regulate anaerobic digestion? Renew Sustain Energy Rev 150:111453. https://doi.org/10.1016/J.RSER.2021.111453
Nitschke M, Pastore GM (2006) Production and properties of a surfactant obtained from Bacillus subtilis grown on cassava wastewater. Bioresour Technol 97:336–341. https://doi.org/10.1016/J.BIORTECH.2005.02.044
Nualsri C, Kongjan P, Reungsang A (2016) Direct integration of CSTR-UASB reactors for two-stage hydrogen and methane production from sugarcane syrup. Int J Hydrog Energy 41:17884–17895. https://doi.org/10.1016/j.ijhydene.2016.07.135
Oh YK, Seol EH, Lee EY, Park S (2002) Fermentative hydrogen production by a new chemoheterotrophic bacterium Rhodopseudomonas palustris P4. Int J Hydrog Energy 27:1373–1379. https://doi.org/10.1016/S0360-3199(02)00100-3
Oliveira FC, Coelho ST (2017) History, evolution, and environmental impact of biodiesel in Brazil: a review. Renew Sustain Energy Rev 75:168–179. https://doi.org/10.1016/j.rser.2016.10.060
Oliveira CA, Fuess LT, Soares LA, Damianovic MHRZ (2020) Thermophilic biohydrogen production from sugarcane molasses under low pH: metabolic and microbial aspects. Int J Hydrog Energy 45:4182–4192. https://doi.org/10.1016/J.IJHYDENE.2019.12.013
O-Thong S, Mamimin C, Kongjan P, Reungsang A (2019) Thermophilic fermentation for enhanced biohydrogen production. Elsevier B.V., Amsterdam
Pandey AK, Pilli S, Bhunia P et al (2022) Dark fermentation: production and utilization of volatile fatty acid from different wastes: a review. Chemosphere 288:132444. https://doi.org/10.1016/j.chemosphere.2021.132444
Peixoto G, Pantoja-Filho JLR, Agnelli JAB et al (2012) Hydrogen and methane production, energy recovery, and organic matter removal from effluents in a two-stage fermentative process. Appl Biochem Biotechnol 168:651–671. https://doi.org/10.1007/s12010-012-9807-4
Rademacher A, Nolte C, Schönberg M, Klocke M (2012) Temperature increases from 55 to 75 °C in a two-phase biogas reactor result in fundamental alterations within the bacterial and archaeal community structure. Appl Microbiol Biotechnol 96:565–576. https://doi.org/10.1007/s00253-012-4348-x
Ramos LR, Silva EL (2017) Continuous hydrogen production from agricultural wastewaters at thermophilic and hyperthermophilic temperatures. Appl Biochem Biotechnol 182:846e69. https://doi.org/10.1007/s12010-016-2366-3
Ramos LR, Silva EL (2018) Continuous hydrogen production from cofermentation of sugarcane vinasse and cheese whey in a thermophilic anaerobic fluidized bed reactor. Int J Hydrog Energy 43:13081–13089. https://doi.org/10.1016/j.ijhydene.2018.05.070
Ramos LR, Silva EL (2020) Thermophilic hydrogen and methane production from sugarcane stillage in two-stage anaerobic fluidized bed reactors. Int J Hydrog Energy 45:5239–5251. https://doi.org/10.1016/J.IJHYDENE.2019.05.025
Ramos LR, Lovato G, Rodrigues JAD, Silva EL (2021) Anaerobic digestion of vinasse in fluidized bed reactors: Process robustness between two-stage thermophilic-thermophilic and thermophilic-mesophilic systems. J Clean Prod 314:128066. https://doi.org/10.1016/j.jclepro.2021.128066
Ren NQ, Chua H, Chan SY et al (2007) Assessing optimal fermentation type for bio-hydrogen production in continuous-flow acidogenic reactors. Bioresour Technol 98:1774–1780. https://doi.org/10.1016/j.biortech.2006.07.026
Ryue J, Lin L, Liu Y et al (2019) Comparative effects of GAC addition on methane productivity and microbial community in mesophilic and thermophilic anaerobic digestion of food waste. Biochem Eng J 146:79–87. https://doi.org/10.1016/J.BEJ.2019.03.010
Saady NMC (2013) Homoacetogenesis during hydrogen production by mixed cultures dark fermentation: unresolved challenge. Int J Hydrog Energy 38:13172–13191. https://doi.org/10.1016/j.ijhydene.2013.07.122
Sánchez AS, Silva YL, Kalid RA et al (2017) Waste bio-refineries for the cassava starch industry: New trends and review of alternatives. Renew Sustain Energy Rev 73:1265–1275. https://doi.org/10.1016/j.rser.2017.02.007
Santos SC, Rosa PRF, Sakamoto IK et al (2014a) Hydrogen production from diluted and raw sugarcane vinasse under thermophilic anaerobic conditions. Int J Hydrog Energy 39:9599–9610. https://doi.org/10.1016/j.ijhydene.2014.04.104
Santos SC, Rosa PRF, Sakamoto IK et al (2014b) Organic loading rate impact on biohydrogen production and microbial communities at anaerobic fluidized thermophilic bed reactors treating sugarcane stillage. Bioresour Technol 159:55–63. https://doi.org/10.1016/j.biortech.2014.02.051
Shida GM, Barros AR, dos Reis CM et al (2009) Long-term stability of hydrogen and organic acids production in an anaerobic fluidized-bed reactor using heat treated anaerobic sludge inoculum. Int J Hydrog Energy 34:3679–3688. https://doi.org/10.1016/J.IJHYDENE.2009.02.076
Silva FMS, Oliveira LB, Mahler CF, Bassin JP (2017) Hydrogen production through anaerobic co-digestion of food waste and crude glycerol at mesophilic conditions. Int J Hydrog Energy 42:22720–22729. https://doi.org/10.1016/j.ijhydene.2017.07.159
Singh V, Das D (2019) Chapter3 potential of hydrogen production from biomass. Academic Press, London, pp 123–164
Sivagurunathan P, Kumar G, Bakonyi P et al (2016) A critical review on issues and overcoming strategies for the enhancement of dark fermentative hydrogen production in continuous systems. Int J Hydrog Energy 41:3820–3836. https://doi.org/10.1016/j.ijhydene.2015.12.081
Sugiarto Y, Sunyoto NMS, Zhu M et al (2021) Effect of biochar in enhancing hydrogen production by mesophilic anaerobic digestion of food wastes: the role of minerals. Int J Hydrog Energy 46:3695–3703. https://doi.org/10.1016/J.IJHYDENE.2020.10.256
Taheri E, Amin MM, Pourzamani H et al (2018) Comparison of acetate-butyrate and acetate-ethanol metabolic pathway in biohydrogen production. J Med Signals Sens 8:101–107
Watthier E, Andreani CL, Torres DGB et al (2019) Cassava wastewater treatment in fixed-bed reactors: organic matter removal and biogas production. Front Sustain Food Syst. https://doi.org/10.3389/FSUFS.2019.00006
Wosiacki G, Cereda MP (2002) Valorização De Resíduos Do Processamento De Mandioca. Publ UEPG - Cienc Exatas e Da Terra, Agrar e Eng 8:27–43. https://doi.org/10.5212/publicatio.v8i01.762
Wu KJ, Chang CF, Chang JS (2007) Simultaneous production of biohydrogen and bioethanol with fluidized-bed and packed-bed bioreactors containing immobilized anaerobic sludge. Process Biochem 42:1165–1171. https://doi.org/10.1016/j.procbio.2007.05.012
Xie L, Liu H, Chen Y, Zhou Q (2014) pH-adjustment strategy for volatile fatty acid production from high-strength wastewater for biological nutrient removal. Water Sci Technol 69:2043–2051. https://doi.org/10.2166/wst.2014.120
Zhang K, Ren N-Q, Wang A-J (2015) Fermentative hydrogen production from corn stover hydrolyzate by two typical seed sludges: Effect of temperature. Int J Hydrog Energy 40:3838–3848. https://doi.org/10.1016/j.ijhydene.2015.01.120
Zhu H, Parker W, Basnar R et al (2009) Buffer requirements for enhanced hydrogen production in acidogenic digestion of food wastes. Bioresour Technol 100:5097–5102. https://doi.org/10.1016/j.biortech.2009.02.066
Zou L, Wang C, Zhao X et al (2021) Enhanced anaerobic digestion of swine manure via a coupled microbial electrolysis cell. Bioresour Technol 340:125619. https://doi.org/10.1016/J.BIORTECH.2021.125619
Funding
This research was funded by conselho nacional de desenvolvimento científico e tecnológico.
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
de Gois Araújo Tavares, T., Peiter, F.S., Chaves, T.C. et al. Effect of thermophilic temperatures on hydrogen and ethanol production in anaerobic fluidized bed reactor from cassava wastewater. Braz. J. Chem. Eng. 40, 115–127 (2023). https://doi.org/10.1007/s43153-022-00222-w
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s43153-022-00222-w