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Chromosomes polymorphisms of Sichuan wheat cultivars displayed by ND-FISH landmarks

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Abstract

Although fluorescence in situ hybridization (FISH) karyotypes of wheat chromosomes have already been widely investigated, more probes are needed to enrich them. Novel karyotype characteristics of 166 common wheat cultivars bred from the hometown of Chinese Spring were revealed using 11 oligonucleotide (oligo) probes derived from nine kinds of tandem repeats. Chromosomes 5A, 3B and 1D showed the highest number (24, 18 and 6) of karyotype variations in A-, B-, and D-genome chromosomes, respectively. According to the FISH karyotypes, the analyzed wheat cultivars were divided into eight clusters that correspond to breeding entities. Thirty and 38 cultivars contain wheat-Haynaldia villosa 6AL.6VS and wheat-rye 1BL.1RS translocation chromosomes, respectively, indicating their high breeding value in Sichuan, China. Additionally, four and three types of 6AL.6VS and 1BL.1RS translocations were observed, respectively. Scarce translocations were detected. 'Neimai' series cultivars contain reciprocal translocations 5AS.3BS/5AL.3BL. Three cultivars contain reciprocal translocations 5BL.5BS-7BL/7BS.7BL-5BS that occurred at high frequency in European wheat cultivars, indicating that the translocation is also adapted to Sichuan environments. These results indicate that some special karyotypes exist in Sichuan wheat cultivars, showing the relationship between karyotype and adaptation. Richer FISH bands of wheat chromosomes were revealed, and this can be used to investigate meiotic recombination conveniently.

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Acknowledgements

We are thankful to Ennian Yang, Ling Wu, Zongjun Pu (Crop Research Institute, Sichuan Academy of Agricultural Sciences, China;), Yong Ren (Mianyang Branch of National Wheat Improvement Center, Mianyang Institute of Agricultural Sciences, China), Tao Wang (Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China) and Suizhuang Yang (School of Life Science and Engineering, Southwest University of Science and Technology, China) for their kindly providing seeds. This manuscript is provided by the National Natural Science Foundation of China (No. 32070373), the Science and Technology Project of Sichuan, China (No. 2020YJ0128) and the “13th Five-Year” Crops, Livestock and Poultry Breeding Program of Sichuan Province (No. 2016NY0030).

Funding

This manuscript is provided by the National Natural Science Foundation of China (No. 32070373), the Science and Technology Project of Sichuan, China (No. 2020YJ0128) and the “13th Five-Year” Crops, Livestock and Poultry Breeding Program of Sichuan Province (No. 2016NY0030).

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Author notes

  1. Zhenglei Hu and Jiangtao Luo have contributed equally to this work.

Authors and Affiliations

  1. College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China

    Zhenglei Hu, Linrong Wan, Jie Luo, Shulan Fu & Zongxiang Tang

  2. Provincial Key Laboratory for Plant Genetics and Breeding, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China

    Zhenglei Hu, Linrong Wan, Jie Luo, Shulan Fu & Zongxiang Tang

  3. Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China

    Jiangtao Luo

  4. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China

    Yazhou Li, Dengcai Liu & Ming Hao

Authors
  1. Zhenglei Hu
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  2. Jiangtao Luo
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  3. Linrong Wan
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  4. Jie Luo
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  5. Yazhou Li
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  8. Ming Hao
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  9. Zongxiang Tang
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Contributions

SF, ZT, MH, and DL conceived and designed the study. ZT, JL and MH collected materials. ZH, JL, LW and JL performed the experiments. ZH, JL, LW and MH analyzed data. ZT wrote original draft preparation. SF, MH, and DL reviewed and edited this manuscript.

Corresponding authors

Correspondence to Ming Hao or Zongxiang Tang.

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Additional information

Communicated by M. Molnár-Láng.

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Hu, Z., Luo, J., Wan, L. et al. Chromosomes polymorphisms of Sichuan wheat cultivars displayed by ND-FISH landmarks. CEREAL RESEARCH COMMUNICATIONS 50, 253–262 (2022). https://doi.org/10.1007/s42976-021-00173-x

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  • DOI: https://doi.org/10.1007/s42976-021-00173-x

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