Abstract
The wheat spike mutant 'three-pistil' (TP) exhibits normal external morphology but produces two additional pistils per floret. Much attention has been paid to this natural mutation because elucidation of its genetic foundation potentially paves a way to increase the number of grains per spike for breeding. Our previous studies roughly mapped the causal locus of TP on chromosome 2D. In this study, we performed genetic mapping of the three-pistil gene Pis1 in an F2 population derived from a cross of TP and synthetic hexaploid wheat of which chromosome 2D was from Aegilops tauschii. Segregation analysis in the F2 population confirmed the dominance of the Pis1 locus. Bulked segregant analysis with three DNA marker systems revealed a total of 18 markers genetically linked with Pis1. Successive genetic linkage analysis of the F2 individuals using the 18 markers identified two sequence-related amplified polymorphisms markers, Me5-eM33 and Me26-eM33, flanked the Pis1 locus at distances of 0.6 and 1.7 cM, respectively. Integration of the genetic linkage and previously discovered DNA markers associated with Pis1 revealed a potential location of the Pis1 locus between Me5-eM33 and a KASP marker M75. Thus, the constructed partial genetic linkage map is useful for the isolation of Pis1 and marker-assisted breeding in wheat.
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References
Blake VC, Woodhouse MR, Lazo GR, Odell SG, Wight CP, Tinker NA, Wang Y, Gu YQ, Birkett CL, Jannink JL, Matthews DE, Hane DL, Michel SL, Yao E, Sen TZ (2019) GrainGenes: centralized small grain resources and digital platform for geneticists and breeders. Database (Oxford). pii: baz065
Camacho C, Coulouris G, Avagyan V, Avagyan V, Ma N, Papadopoulos B, Madden TL (2009) BLAST + : architecture and applications. BMC Bioinf 10:421
FAO (2017) Food and agriculture organization of united nations. Retrieved from http://www.fao.org/faostat/zh/#data/QC. Accessed 3 July 2020
Kosambi D (1994) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103:455–461
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Peng ZS (2003) A new mutation in wheat producing three pistils in a floret. J Agron Crop Sci 189:270–272
Peng ZS, Martinek P, Kosuge K, Kuboyama T, Watanabe N (2008) Genetic mapping of a mutant gene producing three pistils per floret in common wheat. J Appl Genet 49:135–139
Shitsukawa N, Kinjo H, Takumi S, Murai K (2009) Heterochronic development of the floret meristem determines grain number per spikelet in diploid, tetraploid and hexaploid wheats. Ann Bot 104:243–251
Van Ooijen JW (2006) JoinMap® 4, software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, Wageningen, Netherlands
Wang XB, Ma CX, He KQ, Si HQ, Zhang YL (2008) Development and application of a STS marker for grain PPO gene located on chromosome 2D in common wheat. Scientia Agricultura Sinica 41:1583–1590
Wang Z, Xu D, Ji J, Wang J, Wang M, Ling H, Sun G, Li J (2009) Genetic analysis and molecular markers associated with multi-gynoecia (Mg) gene in Trigrain wheat. Can J Plant Sci 89:845–850
Yang ZJ, Peng ZS, Yang H, Yang J, Wei SH, Cai P (2011) Suppression subtractive hybridization identified differentially expressed genes in pistil mutations in wheat. Plant Mol Biol Rep 29:431–439
Yang ZJ, Chen ZY, Peng ZS, Yu Y, Liao ML, Wei SH (2017) Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers. BMC Genom 18:567
Zhao LB, Zhang L, Qu JP, Yu Y, Lu L, Peng ZS, Yang WY, Wei SH, Yang J, Yang ZJ (2017) Novel fluorescent sequence-related amplified polymorphism (FSRAP) markers for the construction of a genetic linkage map of wheat (Triticum aestivum L.). Genetika-Belgrade 49:1081–1093
Zhou Q, Yuan ZW, Zhang LQ, Ning SZ, Ren Y, Tao J, Li SR, Liu DC (2015) Genetic analysis on dwarfing trait in landrace ailanmai of Triticum turgidum L. ssp. Turgidum Acta Agronomic Sinica 41:1899–1905
Zhu XX, Ni YJ, He RS, Jiang YM, Li QY, Niu JS (2019) Genetic mapping and expressivity of a wheat multi-pistil gene in mutant 12TP. J Integr Agric 18:532–538
Acknowledgements
This research was supported by the National Natural Science Foundation of China (Grant number 31760425), the National General Cultivation Project of China West Normal University (Grant number 17C043) the project of Youth Science and Technology Innovation Team of Sichuan Province, China (Grant No. 2017TD0008). The Syn-SAU-1 seeds were provided by research fellow Dengcai Liu, Sichuan Agricultural University.
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Communicated by E. Khlestkina.
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Yu, Z.Y., Luo, Q., Peng, Z.S. et al. Genetic mapping of the three-pistil gene Pis1 in an F2 population derived from a synthetic hexaploid wheat using multiple molecular marker systems. CEREAL RESEARCH COMMUNICATIONS 49, 31–36 (2021). https://doi.org/10.1007/s42976-020-00078-1
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DOI: https://doi.org/10.1007/s42976-020-00078-1