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Effect of ginger essential oil and 6-gingerol on a multispecies biofilm of Listeria monocytogenes, Salmonella Typhimurium, and Pseudomonas aeruginosa

  • Food Microbiology - Research Paper
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Abstract

The objective of this study was to evaluate the potential antimicrobial and antibiofilm effect of ginger essential oil (GEO) and 6-gingerol on a multispecies biofilm formed by Listeria monocytogenes, Salmonella Typhimurium, and Pseudomonas aeruginosa on a polypropylene surface. The minimum inhibitory concentration concentrations obtained for GEO were 100 and 50 mg/mL and for 6-gingerol 1.25 mg/mL. Sessile cell counts ranged within 5.35–7.35 log CFU/cm2 in the control biofilm, with the highest sessile growth at 72 h. GEO treatments acted on the total population regardless of concentration at 1 and 48 h. L. monocytogenes behaved similarly to the total population, showing GEO action at 1 h and keeping the same pattern at 48, 72, and 96 h. Better action on S. Typhimurium was obtained at times of 1, 72, and 96 h. P. aeruginosa showed logarithmic reduction only when treated with GEO 50 mg at 24 h. As for 6-gingerol, in general, there was no significant action (p > 0.05) on the evaluated sessile cells. GEO showed antimicrobial activity against L. monocytogenes, S. Typhimurium, and P. aeruginosa, acting as an inhibitor of biofilm formation. As for 6-gingerol, it was considered a possible antimicrobial agent but without efficacy during biofilm formation.

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Acknowledgements

The authors thank the National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq, Brasília, DF, Brazil), the Coordination for the Improvement of Higher Education Personnel (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES, Brasília, DF, Brazil—Code 001 and CAPES PRINT Project, 88881310254/2018-01), and the Federal University of Paraná (Universidade Federal do Paraná—UFPR; nº 02/2020—RESEARCH/PRPPG/UFPR—Araucária Foundation).

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Authors and Affiliations

  1. School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Distrito de Rubião Jr, BotucatuSão Paulo, SN, 18618-970, Brazil

    Emanoelli Aparecida Rodrigues dos Santos, Leonardo Ereno Tadielo, Fábio Sossai Possebon & Juliano Gonçalves Pereira

  2. Department of Veterinary Sciences, Federal University of Paraná, Palotina Campus, Rua Pioneiro, Jardim Dallas, Palotina, PR, 215385950-000, Brazil

    Jhennifer Arruda Schmiedt & Luciano dos Santos Bersot

  3. Biological Engineering Center, University of Minho, Gualtar Campus, 4710–057, Braga, Portugal

    Maria Olivia Pereira

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  1. Emanoelli Aparecida Rodrigues dos Santos
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Contributions

EARS: conceptualization, methodology, data curation, writing—original draft, writing—preparation of the original draft. LET: methodology, visualization, investigation. JAS: methodology, visualization, investigation. FSB: formal analysis, writing—review and editing, writing—revision and editing. MOP: formal analysis, writing—review and editing, writing—revision and editing. JGP: formal analysis, writing—review and editing, writing—revision and editing. LSB: supervision, project administration, writing—review and editing, writing, revision and editing.

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Correspondence to Luciano dos Santos Bersot.

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dos Santos, E.A.R., Tadielo, L.E., Schmiedt, J.A. et al. Effect of ginger essential oil and 6-gingerol on a multispecies biofilm of Listeria monocytogenes, Salmonella Typhimurium, and Pseudomonas aeruginosa. Braz J Microbiol 54, 3041–3049 (2023). https://doi.org/10.1007/s42770-023-01075-2

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