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Evaluation of phenotypic detection of carbapenemase-producing Pseudomonas spp. from clinical isolates

  • Clinical Microbiology - Research Paper
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Abstract

Carbapenems are considered last-resort antibiotics for the treatment of infections caused by multidrug-resistant Gram-negative bacteria. Although the main mechanism of carbapenem-resistance in Pseudomonas aeruginosa is the loss of OprD porin, carbapenemases continue to be a problem worldwide. The aim of this study was to evaluate the performance of phenotypic tests (Carba NP, Blue Carba, and mCIM/eCIM) for detection of carbapenemase-producing Pseudomonas spp. in Brazil. One hundred twenty-seven Pseudomonas spp. clinical isolates from different Brazilian states were submitted to phenotypic and molecular carbapenemase detection. A total of 90 carbapenemase-producing P. aeruginosa and 5 Pseudomonas putida (35, blaVIM-2; 17, blaSPM-1; 2, blaIMP-10; 1, blaVIM-24; 1, blaNDM-1; 39, blaKPC-2). The phenotypic Carba NP, Blue Carba, and mCIM/eCIM showed sensitivity of 94.7%, 93.6%, and 93.6%, and specificity of 90.6%, 100%, and 96.8%, respectively. However, only the Carba NP presented the highest sensitivity and showed the ability in differentiating the carbapenemases between class A and class B using EDTA. Blue Carba failed to detect most of the class B carbapenemases, having the worst performance using EDTA. Our results show changes in the epidemiology of the spread of carbapenemases and the importance of their detection by phenotypic and genotypic tests. Such, it is essential to use analytical tools that faithfully detect bacterial resistance in vitro in a simple, sensitive, rapid, and cost-effective way. Much effort must be done to improve the current tests and for the development of new ones.

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Acknowledgements

We thank the PDTIS-IOC DNA Sequencing Platform for DNA sequencing.

Funding

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Carlos Chagas de Amparo à Pesquisa (FAPERJ), and Instituto Oswaldo Cruz (IOC)—Fiocruz.

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  1. Laboratório de Pesquisa Em Infecção Hospitalar (LAPIH), Instituto Oswaldo Cruz - FIOCRUZ, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ, 21045900, Brazil

    Ivson Cassiano de Oliveira Santos, Orlando Carlos da Conceiçāo Neto, Bianca Santos da Costa, Camila Bastos Tavares Teixeira, Leilane da Silva Pontes, Melise Chaves Silveira, Cláudio Marcos Rocha-de-Souza & Ana Paula D’Alincourt Carvalho-Assef

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  1. Ivson Cassiano de Oliveira Santos
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de Oliveira Santos, I.C., da Conceiçāo Neto, O.C., da Costa, B.S. et al. Evaluation of phenotypic detection of carbapenemase-producing Pseudomonas spp. from clinical isolates. Braz J Microbiol 54, 135–141 (2023). https://doi.org/10.1007/s42770-022-00857-4

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