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Bioprospecting and selection of tolerant strains and productive analyses of microalgae grown in vinasse

  • Environmental Microbiology - Research Paper
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Abstract

In order to contribute to the biotechnology of microalgae cultivated in vinasse, we carried out the bioprospection of tolerant species and synthesized biomolecules of the total biomass (microalgae and bacteria), recovered from cultures. To use vinasse as a culture medium for the microalgae, waste was centrifuged and used in concentrations from 5 to 50%. Daily cell densities, growth rates, and EC50 values were obtained. After defining the best pair of vinasse concentration/microalgae strain, dry biomass, and composition (proteins and carbohydrates) were determined in 96 h cultures, considering the associated community (bacteria and yeast). The microalgae tested were Chlamydomonas sp., Chlorella sorokiniana, Chlorella vulgaris, Desmodesmus spinosus, Haematococcus pluvialis, Monoraphidium sp., Scenedesmus quadricauda, and Tetraselmis gracilis. The results showed that although the microalgal growth rates in vinasse were similar to controls in BG11, the cells in vinasse had higher biovolumes, dry biomass, and total proteins. The species H. pluvialis, S. quadricauda, and T. gracilis showed the best productivity parameters in vinasse, despite lower growth rates than the other species. Using low concentrations of centrifuged vinasse as a culture medium, only 22% of biological contaminants were present, thus most of the processed biomass was mainly composed of microalgae. Thus, Chlamydomonas sp., D. spinosus, S. quadricauda, and H. pluvialis microalgae have attributes such as resistance and biomolecules that make them candidates for further optimization in production systems, combining the environmental benefits of using waste with the production of biomolecules and/or biomass of commercial interest.

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Acknowledgements

This study received support from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES–Brasil, Finance Code 001), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq–Brasil Proc. No. 304280/2019-4) and the São Paulo Research Foundation (FAPESP Process 2018/07988-5). We would like to thank Prof. Dr. Clóvis Wesley de Souza (DMP UFSCar) for his contribution in isolating the algal strains used in the initial experiments.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ecologia e Recursos Naturais (PPGERN), Universidade Federal de São Carlos (UFSCar), Rod. Washington Luís km 235, São Paulo, São Carlos, CEP 13565-905, Brazil

    Camila Candido

  2. Departamento de Botânica (DB), Universidade Federal de São Carlos (UFSCar), Rod. Washington Luís km 235, São Paulo, São Carlos, CEP 13565-905, Brazil

    Lucas Guimarães Cardoso & Ana Teresa Lombardi

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  1. Camila Candido
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  2. Lucas Guimarães Cardoso
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  3. Ana Teresa Lombardi
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Contributions

C Candido: conceptualization, investigation, formal analysis, writing—original draft, review, editing, and graphics. L G Cardoso: conceptualization, writing—original draft, review, editing, and graphics. A T Lombardi: conceptualization, funding acquisition, and supervision. All authors read and approved the final manuscript.

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Correspondence to Lucas Guimarães Cardoso.

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Candido, C., Cardoso, L.G. & Lombardi, A.T. Bioprospecting and selection of tolerant strains and productive analyses of microalgae grown in vinasse. Braz J Microbiol 53, 845–855 (2022). https://doi.org/10.1007/s42770-022-00692-7

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