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Soybean tolerance to drought depends on the associated Bradyrhizobium strain

  • Environmental Microbiology - Research Paper
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Abstract

We evaluated the effect of three different Bradyrhizobium strains inoculated in two soybean genotypes (R01-581F, drought-tolerant, and NA5858RR, drought-sensitive) submitted to drought in two trials conducted simultaneously under greenhouse. The strains (SEMIA 587, SEMIA 5019 (both B. elkanii), and SEMIA 5080 (B. diazoefficiens)) were inoculated individually in each genotype and then submitted to water restriction (or kept well-watered, control) between 45 and 62 days after emergence. No deep changes in plant physiological variables were observed under the moderate water restriction imposed during the first 10 days. Nevertheless, photosynthesis and transpiration decreased after the severe water restriction imposed for further 7 days. Water restriction reduced growth (− 30%) and the number of nodules (− 47% and − 58% for R01-581F and NA5858RR, respectively) of both genotypes, with a negative effect on N-metabolism. The genotype R01-581F inoculated with SEMIA 5019 strain had higher photosynthetic rates compared with NA5858RR, regardless of the Bradyrhizobium strain. On average, R01-581F showed better performance under drought than NA5858RR, with higher number of nodules (51 vs. 38 nodules per plant, respectively) and less accumulation of ureides in petioles (15 μmol g−1 vs. 34 μmol g−1, respectively). Moreover, plants inoculated with SEMIA 5080 had higher glutamine synthetase activity under severe water restriction, especially in the drought-tolerant R01-518F, suggesting maintenance of N metabolism under drought. The Bradyrhizobium strain affects the host plant responses to drought in which the strain SEMIA 5080 improves the drought tolerance of R01-518F genotype.

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Acknowledgments

P. Cerezini thanks a PhD fellowship from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior); M.A. Nogueira and M. Hungria are CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) research fellows. Financed by Embrapa (02.13.08.003.00.00). The group belongs to the INCT-Plant-Growth Promoting Microorganisms for Agricultural Sustainability and Environmental Responsibility (465133/2014-2)—Fundação Araucária.

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Authors and Affiliations

  1. Department of Agronomy, Universidade Estadual de Londrina, C. Postal 10.011, Londrina, PR, 86057-970, Brazil

    Paula Cerezini & Biana Harumi Kuwano

  2. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brasília, DF, Brazil

    Paula Cerezini & Anna Karolina Grunvald

  3. Embrapa Soja, C. Postal 231, Londrina, PR, 86001-970, Brazil

    Mariangela Hungria & Marco Antonio Nogueira

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  1. Paula Cerezini
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  2. Biana Harumi Kuwano
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Correspondence to Marco Antonio Nogueira.

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Cerezini, P., Kuwano, B.H., Grunvald, A.K. et al. Soybean tolerance to drought depends on the associated Bradyrhizobium strain. Braz J Microbiol 51, 1977–1986 (2020). https://doi.org/10.1007/s42770-020-00375-1

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