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Genetic identification of pestiviruses from beef cattle in Southern Brazil

  • Veterinary Microbiology - Research Paper
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Abstract

Bovine pestiviruses, e.g., bovine viral diarrhea virus types 1 (BVDV-1 or Pestivirus A), BVDV-2 (Pestivirus B), and HoBi-like pestiviruses (HoBiPeV or Pestivirus H), have been shown to circulate in Brazilian cattle in varied proportions. In this study, we identified genetically pestiviruses circulating in beef cattle in Rio Grande do Sul, the southern most Brazilian state. Screening of serum of 15.584 beef calves destined to be export by an antigen capture ELISA and, subsequently, by reverse-transcription polymerase chain reaction (RT-PCR), revealed 135 containing pestivirus RNA. Genetic typing of these viruses based on nucleotide sequencing and phylogenetic analysis of the 5′ untranslated region (5′ UTR) of the viral genome allowed for the identification of 90 different viruses, being 38 BVDV-1 (42.2%), 31 BVDV-2 (34.4%), and 21 HoBiPeV (23.4%). Among BVDV-1, only subtypes BVDV-1a (n = 28, 31.1%) and BVDV-1b (n = 10, 11.1%) were identified. All 31 BVDV-2 isolates belonged to BVDV-2b subtype and the 21 HoBiPeV viruses clustered to subgroup 3a. Thus, this study provides an approximate genetic profile of pestiviruses circulating in beef cattle in a traditional Brazilian beef cattle-raising state.

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Funding

FLM, RW, and EFF are recipients of CNPq fellowships (Brazilian Council for Research). JFC is recipient of PNPD/CAPES fellowship (Programa Nacional de Pós-Doutorado/Coordenação de Aperfeiçoamento de Pessoal de Nível Superior).

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Authors and Affiliations

  1. Departamento de Medicina Veterinária Preventiva (DMVP), Setor de Virologia (SV), Universidade Federal de Santa Maria (UFSM), Avenida Roraima, 1000, prédio 63A, Centro de Eventos, Santa Maria, RS, 97105-900, Brazil

    Francielle Liz Monteiro, Bruno Martins, Juliana F. Cargnelutti, Jessica G. Noll, Rudi Weiblen & Eduardo F. Flores

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  1. Francielle Liz Monteiro
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  2. Bruno Martins
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  3. Juliana F. Cargnelutti
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  6. Eduardo F. Flores
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Correspondence to Eduardo F. Flores.

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Monteiro, F.L., Martins, B., Cargnelutti, J.F. et al. Genetic identification of pestiviruses from beef cattle in Southern Brazil. Braz J Microbiol 50, 557–563 (2019). https://doi.org/10.1007/s42770-019-00058-6

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  • DOI: https://doi.org/10.1007/s42770-019-00058-6

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