Abstract
Alfalfa (Medicago sativa L.) is one of the major forage legumes grown in Oman, but its productivity is significantly affected by salt stress. In this study, 20 local Omani alfalfa landraces were characterized for morphological and biochemical traits, tissue minerals, and genetic diversity. The experiment was conducted in a greenhouse with three salinity levels: control (2 dS m−1), moderate salinity stress (6 dS m−1), and severe salinity stress (10 dS m−1). Different morphological and biochemical traits and tissue minerals showed significant variation in response to varying salinity levels. With an increase in salinity stress levels from 2 to 10 dS m−1, there was a decrease in different morphological traits across the landraces, except for forage fresh weight which increased at both levels of salt stress (6 and 10 dS m−1) compared to control owing to high leaf free proline, total soluble phenolics, catalase activity, and leaf potassium concentration. However, different biochemical traits and sodium and chloride increased with the increase in salt stress levels (6 to 10 dS m−1) and the higher values were recorded at higher salt stress level (10 dS m−1). The principal component analysis (PCA) had 47.8 and 49.2% variability for morphological traits under salt stress levels of 6 and 10 dS m−1, respectively. Under both levels of salt stress, there was 81% variability by the two PCs for different biochemical traits. The total variability contributed by the two PCs for mineral nutrients was 69.2 and 66.2% for salt stress levels of 6 and 10 dS m−1, respectively. At both levels of salt stress, the landraces OMA 5, OMA 21, OMA 161, OMA 6, OMA 95, OMA 220, and OMA 285 were grouped on high morpho-biochemical traits and tissue mineral contents. There was a low to moderate level of genetic diversity (H = 0.3252) in the Omani alfalfa populations. However, the level of genetic diversity for the populations from the four regions was moderate (Dhahira) to high (Batinah, Dakiliya, and Sharqiyah). The substantial variation among the landraces on morpho-biochemical traits and tissue minerals can be used in the breeding programs to develop salt-tolerant alfalfa genotypes. The landraces OMA 6, OMA 161, and OMA 285 were excellent in performance based on morphological and biochemical traits and tissue mineral contents under both levels of salt stress.
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Al-Farsi SM, Nadaf SK, Al-Sadi AM, Ullah A, Farooq M (2020b) Evaluation of indigenous Omani alfalfa landraces for morphology and forage yield under different levels of salt stress. Physiol Mol Biol Plants 26:1763–1772. https://doi.org/10.1007/s12298-020-00856-5
Al-Farsi SM, Nawaz A, Nadaf SK, Al-Sadi AM, Siddique KHM, Farooq M (2020a) Effects, tolerance mechanisms and management of salt stress in lucerne (Medicago sativa). Crop Pasture Sci 71:411–428. https://doi.org/10.1071/CP20033
Al-Khatib M, McNeilly T, Collins JC (1992) The potential of selection and breeding for improved salt tolerance in lucerne (Medicago sativa L.). Euphytica 65:43–51. https://doi.org/10.1007/BF00022198
Al-Khatib M, McNeilly T, Collins JC (1994) Between and within cultivar variability in salt tolerance in lucerne (Medicago sativa L.). Genetic Res Crop Evol 41:159–164. https://doi.org/10.1007/BF00051632
Al-Maamari IT, Al-Sadi AM, Al-Saady NA (2014) Assessment of genetic diversity in fenugreek (Trigonella foenumgraecum) in Oman. Intl J Agric Biol 16:813–818
Al-Sadi AM, Al-Ghaithi AG, Al-Balushi ZM, Al-Jabri AH (2012) Analysis of diversity in Pythium aphanidermatum populations from a single greenhouse reveals phenotypic and genotypic changes over 2006 to 2011. Plant Dis 96:852–858. https://doi.org/10.1094/PDIS-07-11-0624
Altinok S, Yurtseven E, Avci S, Öztürk HS, Selenay MF (2015) The effects of different irrigation water salinities and leaching ratios on green and dry forage yields of alfalfa (Medicago sativa L.). Poljoprivreda i Sumarstvo 61:85
Ashraf M (2009) Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnol Adv 27:84–93. https://doi.org/10.1016/j.biotechadv.2008.09.003
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
Chance B, Maehly AC (1955) Assay of catalases and peroxidases. Methods Enzymol 2:764–775
Cornacchione MV, Suarez DL (2017) Evaluation of alfalfa (Medicago sativa L.) populations’ response to salinity stress. Crop Sci 57:137–150
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
El-Nakhlawy FS, Shaheen MA, Al-Shareef AR (2012) Response of forage yield, protein and proline contents of alfalfa genotypes to irrigation water salinity and phosphorus fertilizer. J Food Agric Environ 10:551–557. https://doi.org/10.1234/4.2012.2718
Falakboland Z, Zhou M, Zeng F, Kiani-Pouya A, Shabala L, Shabala S (2017) Plant ionic relation and whole-plant physiological responses to waterlogging, salinity and their combination in barley. Func Plant Biol 44:941–953. https://doi.org/10.1071/FP16385
Farissi M, Ghoulam C, Bouizgaren A (2013) Changes in water deficit saturation and photosynthetic pigments of alfalfa populations under salinity and assessment of proline role in salt tolerance. Agric Sci Res J 3:29–35
Farooq M, Gogoi N, Hussain M, Barthakur S, Paul S, Bharadwaj N, Migdadi HM, Alghamdi SS, Siddique KHM (2017) Effects, tolerance mechanisms and management of salt stress in grain legumes. Plant Physiol Biochem 118:199–217. https://doi.org/10.1016/j.plaphy.2017.06.020
Farooq M, Hussain M, Wakeel A, Siddique KHM (2015) Salt stress in maize: effects, resistance mechanisms, and management. A review. Agron Sustain Dev 35:461–481. https://doi.org/10.1007/s13593-015-0287-0
Farooq M, Rehman A, Al-Alawi AKM, Al-Busaidi WM, Lee D-J (2020) Integrated use of seed priming and biochar improves salt tolerance in cowpea. Sci Horti 272:109507. https://doi.org/10.1016/j.scienta.2020.109507
Farooq M, Ullah A, Lee DJ, Alghamdi SS (2018b) Terminal drought-priming improves the drought tolerance in desi and kabuli chickpea. Intl J Agric Biol 30:1129–36. doi: https://doi.org/10.17957/IJAB/15.0623
Farooq M, Ullah A, Lee DJ, Alghamdi SS, Siddique KHM (2018a) Desi chickpea genotypes tolerate drought stress better than kabuli types by modulating germination metabolism, trehalose accumulation, and carbon assimilation. Plant Physiol Biochem 126:47–54. https://doi.org/10.1016/j.plaphy.2018.02.020
Ferreira J, Cornacchione M, Liu X, Suarez D (2015b) Nutrient composition, forage parameters, and antioxidant capacity of alfalfa (Medicago sativa L.) in response to saline irrigation water. Agriculture 5:577–597. https://doi.org/10.3390/agriculture5030577
Ferreira LJ, Azevedo V, Maroco J, Oliveira MM, Santos AP (2015a) Salt tolerant and sensitive rice varieties display differential methylome flexibility under salt stress. PlosOne 10:e0124060. https://doi.org/10.1371/journal.pone.0124060
Flajoulot S, Ronfort J, Baudouin P, Barre P, Huguet T, Huyghe C, Julier B (2005) Genetic diversity among alfalfa (Medicago sativa) cultivars coming from a breeding program, using SSR markers. Theor Appl Genet 111:1420–1429. https://doi.org/10.1007/s00122-005-0074-4
Flowers TJ (2004) Improving crop salt tolerance. J Exp Bot 55:307–319
Ge ZM, Zhang LQ, Yuan L, Zhang C (2014) Effects of salinity on temperature-dependent photosynthetic parameters of a native C-3 and a nonnative C-4 marsh grass in the Yangtze Estuary, China. Photosynthetica 52:484–492. https://doi.org/10.1007/s11099-014-0055-4
Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198. https://doi.org/10.1016/0003-9861(68)90654-1
Hu Y, Barker AV (1999) A single plant tissue digestion for macronutrient analysis. Commun Soil Sci Plant Analys 30:677–687
Julkenen-Titto R (1985) Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics. J Agric Food Chem 33:213–217. https://doi.org/10.1021/jf00062a013
Khoshbakht D, Ramin AA, Baninasab B (2015) Effects of sodium chloride stress on gas exchange, chlorophyll content and nutrient concentrations of nine citrus rootstocks. Photosynthetica 53:241–249. https://doi.org/10.1007/s11099-015-0098-1
Khajeh-Hosseini M, Powell AA, Bingham IJ (2003) The interaction between salinity stress and seed vigor during germination of soybean seeds. Seed Sci Technol 31:715–725. Doi:https://doi.org/10.15258/sst.2003.31.3.20
Khorshidi MB, Yarnia M, Hassanpanah D (2009) Salinity effect on nutrients accumulation in alfalfa shoots in hydroponic condition. J Food Agric Environ 7:787–790
Li R, Shi F, Fukuda K, Yang Y (2010) Effects of salt and alkali stresses on germination, growth, photosynthesis and ion accumulation in alfalfa (Medicago sativa L.). Soil Sci Plant Nutri 56:725–733. https://doi.org/10.1111/j.1747-0765.2010.00506.x
Lobell DB, Ortiz-Monsterio JI, Gurrola FC, Valenzuuela L (2007) Identification of saline soils with multiyear remote sensing of crop yields. Soil Sci Soc Amer J 71:777–783
Larcher W (2003) Physiological plant ecology, 4th edition, Springer-Verlag: Heidelberg/Berlin, Germany; New York, NY, USA
MAF (2014) Agriculture Statistics. Department of Agriculture Statistics, Directorate General of Planning and development, Ministry of Agriculture & Fisheries, Sultanate of Oman
MAF (2017) Agriculture Statistics. Department of Agriculture Statistics, Directorate General of Planning and development, Ministry of Agriculture & Fisheries, Sultanate of Oman
Mohammadi H, Poustini K, Ahmadi A (2008) Root nitrogen remobilization and ion status of two alfalfa (Medicago sativa L.) cultivars in response to salinity stress. J Agron Crop Sci 194:126–134. https://doi.org/10.1111/j.1439-037X.2008.00294.x
Monirifar H, Barghi M (2009) Identification and selection for salt tolerance in alfalfa (Medicago sativa L.) ecotypes via physiological traits. Notulae Scientia Biol 1:63–66
Nawaz A, Farooq M, Ul-Allah S, Gogoi N, Lal R, Siddique KHM (2020) Sustainable soil management for food security in South Asia. J Soil Sci Plant Nutri doi 21:258–275. https://doi.org/10.1007/s42729-020-00358-z
Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci U S A 70:3321–3323. https://doi.org/10.1073/pnas.70.12.3321
Qiang H, Chen Z, Zhang Z, Wang X, Gao H, Wang Z (2015) Molecular diversity and population structure of a worldwide collection of cultivated tetraploid alfalfa (Medicago sativa subsp. sativa L.) germplasm as revealed by microsatellite markers. PLoS One 10:e0124592. https://doi.org/10.1371/journal.pone.0124592
Robinson PH, Grattan SR, Getachew G, Grieve CM, Poss JA, Suarez DL, Benes SE (2004) Biomass accumulation and potential nutritive value of some forages irrigated with saline-sodic drainage water. Anim Feed Sci Technol 111:175–189. https://doi.org/10.1016/S0377-8401(03)00213-X
Sellami S, Le Hir R, Thorpe MR, Aubry E, Wolff N, Vilaine F, Brini F, Dinant S (2019) Arabidopsis natural accessions display adaptations in inflorescence growth and vascular anatomy to withstand high salinity during reproductive growth. Plants 8:1–17. https://doi.org/10.3390/plants8030061
Smith SE (1993) Salinity and the production of alfalfa (Medicago sativa L.). In: Pessrarkli M (ed) Handbook of crop stress. Marcel Dekker Press, New York, pp 431–448
Torabi M, Halim RA, Sinniah UR, Choukan R (2011) Influence of salinity on the germination of Iranian alfalfa ecotypes. Afri J Agric Res 6:4624–4630
Trinchant JC, Boscari A, Spennato G, Van de Sype G, Le Rudulier D (2004) Proline betaine accumulation and metabolism in alfalfa plants under sodium chloride stress. Exploring its compartmentalization in nodules. Plant Physiol 135:1583–1594. https://doi.org/10.1104/pp.103.037556
Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant Sci 151:59–66. https://doi.org/10.1016/S0168-9452(99)00197-1
Vose P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Friters A, Pot J, Paleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414. https://doi.org/10.1093/nar/23.21.4407
Wang WB, Kim YH, Lee HS, Kim KY, Deng XP, Kwak SS (2009a) Analysis of antixidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiol Biochem 47:570–577. https://doi.org/10.1016/j.plaphy.2009.02.009
Wang X, Yang X, Chen L, Feng G, Zhang J, Jin L (2011) Genetic diversity among alfalfa (Medicago sativa L.) cultivars in Northwest China. Acta Agric Scand Sec B- Soil Plant Sci 61:60–67. https://doi.org/10.1080/09064710903496519
Wang Y, Peng X, Salvato F, Wang Y, Yan X, Zhou Z, Lin J (2019) Salt-adaptive strategies in oil seed crop Ricinus communis early seedlings (cotyledon vs. true leaf) revealed from proteomics analysis. Ecotoxicol Environ Safety 171:12–25. https://doi.org/10.1016/j.ecoenv.2018.12.046
Wang YX, Zhang B, Wang T (2009b) Effect of salt stress on the contents of chlorophyll and betaine and its membrane permeability of Medicago sativa [J]. Pratacultural Sci. 3:S541. 9. http://en.cnki.com.cn/Article_en/CJFDTotal-CYKX200903012.htm
Yeh RC, Boyle TJB (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belg J Bot 129:157
Zhanwu G, Hui Z, Jicai G, Chunwu Y, Chunsheng M, Deli W (2011) Germination responses of alfalfa (Medicago sativa L.) seeds to various salt alkaline mixed stress. Afric J Agric Res 6:3793–3803
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The financial support received from Sultan Qaboos University, Oman, through an internal grant (IG/AGR/CROP/19/01) is acknowledged.
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Al-Farsi, S.M., Al-Sadi, A.M., Ullah, A. et al. Salt Tolerance in Alfalfa Landraces of Omani Origin: Morpho-Biochemical, Mineral, and Genetic Diversity Assessment. J Soil Sci Plant Nutr 21, 1484–1499 (2021). https://doi.org/10.1007/s42729-021-00455-7
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DOI: https://doi.org/10.1007/s42729-021-00455-7