Abstract
Nitrogen (N) is the most limiting nutrient for crops growth and development. The optimal application of N significantly enhances crop metabolic process. On the other hand, overuse of nitrogenous fertilizer leads to excess N accumulation in crop tissues that increases the risk of health-related problems. Therefore, it is mandatory to develop a quantitative approach for monitoring the crop N status during growth period. The main objective of this study was to establish the critical nitrogen dilution curve as an effective method to diagnose nitrogen status of sweet basil (Ocimum basilicum L.) grown under controlled greenhouse conditions. Two experiments were carried out with seven N application rates in the research greenhouse of University of Tehran, Iran. Values of crop shoot biomass (t ha−1) and N concentration (%) were measured in each sampling date to develop the critical nitrogen concentration (Nc) equation. The relationship between Nc and shoot dry matter (DM) was described by a power function (Nc = 4.80DM-0.38, R2 = 0.98). The validation results showed that the developed equation effectively distinguishes N-limiting from non-N-limiting treatments. The N nutrition index (NNI) ranged from 0.52 to 1.18 and 0.53 to 1.26 for the first and second experiment, respectively. It was concluded that the developed N dilution curve could provide useful insight into determination of basil N nutrition status and proper fertilizer management.
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References
Amiri M, Eslamian S (2010) Investigation of climate change in Iran. J Environ Sci Technol 3(4):208–216. https://doi.org/10.3923/jest.2010.208.216
Ata-Ul-Karim ST, Yao X, Liu X, Cao W, Zhu Y (2013) Development of critical nitrogen dilution curve of Japonica rice in Yangtze River Reaches. Field Crops Res 149:149–158
Ata-Ul-Karim S, Cao Q, Zhu Y, Tang L, Rehmani M, Cao W (2016) Non-destructive assessment of plant nitrogen parameters using leaf chlorophyll measurements in rice. Front Plant Sci 7. https://doi.org/10.3389/fpls.2016.01829
Ata-Ul-Karim ST, Zhu Y, Lu XJ, Cao Q, Tian YC, Cao W (2017a) Estimation of nitrogen fertilizer requirement for rice crop using critical nitrogen dilution curve. Field Crops Res 201:32–40
Ata-Ul-Karim S, Zhu Y, Cao Q, Rehmani M, Cao W, Tang L (2017b) In-season assessment of grain protein and amylose content in rice using critical nitrogen dilution curve. Eur J Agron 90:139–151. https://doi.org/10.1016/j.eja.2017.08.001
Barker A, Pilbeam D (2015) Handbook of plant nutrition. CRC Press, Boca Raton. https://doi.org/10.1201/b18458
Bélanger G, Walsh JR, Richards JE, Milburn PH, Ziadi N (2001) Critical nitrogen curve and nitrogen nutrition index for potato in eastern Canada. Am J Potato Res 78:355–364. https://doi.org/10.1007/BF02884344
Bufalo J, Cantrell C, Astatkie T, Zheljazkov D, Gawde A, Sílvia Fernandes Boaro C (2015) Organic versus conventional fertilization effects on sweet basil (Ocimum basilicum L.) growth in a greenhouse system. Ind Crop Prod 74:249–254. https://doi.org/10.1016/j.indcrop.2015.04.032
Debaeke P, Rouet P, Justes E (2006) Relationship between the normalized SPAD index and the nitrogen nutrition index: application to durum wheat. J Plant Nutr 29(1):75–92. https://doi.org/10.1080/01904160500416471
Dumas JBA (1831) Procedes de l'analyse organic. Ann Chim Phys 247:198–213
Esfahani M, Abbasi HA, Rabiei B, Kavousi M (2008) Improvement of nitrogen management in rice paddy fields using chlorophyll meter (SPAD). Paddy Water Environ 6:181–188. https://doi.org/10.1007/s10333-0070094-6
Ghaly AE, Ramakrishnan VV (2013) Nitrification of urea and assimilation of nitrate in saturated soils under aerobic conditions. Am J Agric Biol Sci 8(4):330–342. https://doi.org/10.3844/ajabssp.2013.330.342
Hansen PM, Schjoerring JK (2003) Reflectance measurement of canopy biomass and nitrogen status in wheat crops using normalized difference vegetation indices and partial leaves in response to N limitation. Remote Sens Environ 86:542–553. https://doi.org/10.1016/S0034-4257(03)00131-7
Herrmann A, Taube F (2004) The range of the critical nitrogen dilution curve for maize (Zea mays L.) can be extended until silage maturity. Agron J 96:1131–1138
Hoel BO, Solhaug KA (1998) Effect of irradiance on chlorophyll estimation with the Minolta SPAD-502 leaf chlorophyll meter. Ann Bot 82:389–392
Houlés V, Guerif M, Mary BV (2007) Elaboration of a nitrogen nutrition indicator for winter wheat based on leaf area index and chlorophyll content for making nitrogen recommendations. Eur J Agron 27:1–11
Huang S, Miao Y, Cao Q, Yao Y, Zhao G, Yu W, Shen J, Yu K, Bareth G (2018) A new critical nitrogen dilution curve for ricenitrogen status diagnosis in Northeast China. Pedosphere. 28:814–822
Jadon P, Selladurai R, Yadav S, Coumar M, Dotaniya M, Singh A, Bhadouriya J, Kundu S (2018) Volatilization and leaching losses of nitrogen from different coated urea fertilizers. J Soil Sci Plant Nutr 18(4):1036–1047
Jongschaap REE, Booil R (2004) Spectral measurements at different spatial scales in potato: relating leaf, plant and canopy nitrogen status. Int J Appl Earth Obs Geoinf 5:205–218
Justes E, Mary B, Meynard JM, Machet JM, Thelier-Huch’e L (1994) Determination of a critical nitrogen dilution curve for winter wheat crops. Ann Bot 74:397–407
Kjeldahl J (1883) Neue Methode zur Bestimmung des Stickstoffs in organischen Körpern. Fresenius J Anal Chem 22:366–382
Le Bail M, Jeuffroy MH, Bouchard C, Barbotin A (2005) Is it possible to forecast the grain quality and yield of different varieties of winter wheat from Minolta SPAD meter measurements? Eur J Agron 23:379–391
Leghari SJ, Wahocho NA, Laghari GM, Laghari AH, Bhabhan GM, Talpur KH, Bhutto TA, Wahocho SA, Lashari AA (2016) Role of nitrogen for plant growth and development: a review. Adv Environ Biol 10(9):209–218
Lemaire G, Gastal F (1997) N uptake and distribution in plant canopies. In: Lemaire G (ed) Diagnosis of the nitrogen status in crops. Springer-Verlag, Heidelberg, pp 3–43
Lemaire G, Gastal F, Salette J (1989) Analysis of the effect of N nutrition on dry matter yield of a sward by reference to potential yield and optimum N content. XVI International Grassland Congress, Nice, France, p 179–180
Lemaire G, Jeuffroy MH, Gastal F (2008) Diagnosis tool for plant and crop N status invegetative stage: theory and practices for crop N management. Eur J Agron 28(4):614–624
Liu H, Zhu H, Li Z, Yang G (2019) Quantitative analysis and hyperspectral remote sensing of the nitrogen nutrition index in winter wheat. Int J Remote Sens. https://doi.org/10.1080/01431161.2019.1650984
Marotti M, Piccaglia R, Giovanelli E (1996) Differences in essential oil composition of basil (Ocimum basilicum L.): Italian cultivars related to morphological characteristics. J Agric Food Chem 44:3926–3929
Mascarello AC, Silva TRB, Gouveia BT, Bernardi D, Secco D, Santos RF, Alves CZ (2016) Chlorophyll meter reading and total nitrogen content applied as topdressing in parts of the crambe plant. Afr J Biotechnol 15:45–49. https://doi.org/10.5897/AJB2015.14480
Miao Y, Mulla DJ, Randall GW, Vetsch JA, Vintila R (2009) Combining chlorophyll meter readings and high spatial resolution remote sensing images for in-season site-specific nitrogen management of corn. Precis Agric 10:45–62
Ministry of Agriculture Jihad | Iran Data Portal (2019) from http://irandataportal.syr.edu/ministry-of-agriculture
Mistele B, Schmidhalter U (2008) Estimating the nitrogen nutrition index using spectral canopy reflectance measurements. Eur J Agron 29(4):184–190. https://doi.org/10.1016/j.eja.2008.05.007
Muñoz-Huerta RF, Guevara-Gonzalez RG, Contreras-Medina LM, Torres-Pacheco I, Prado-Olivarez J, Ocampo-Velazquez RV (2013) A review of methods for sensing the nitrogen status in plants: advantages, disadvantages and recent advances. Sensors. 13(8):10823–10843. https://doi.org/10.3390/s130810823
Plénet D, Cruz P (1997) The nitrogen requirement of major agricultural crops: maize and sorghum. In: Lemaire G (ed) Diagnosis of the nitrogen status in crops. Springer-Verlag, Heidelberg, pp 93–106
Plénet D, Lemaire G (2000) Relationships between dynamics of nitrogen uptake and dry matter accumulation in maize crops. Determination of critical N concentration. Plant Soil 216:65–82
Qiu W, Wang Z, Huang C, Chen B, Yang R (2014) Nitrate accumulation in leafy vegetables and its relationship with water. J Soil Sci Plant Nutr 14:761–768
Ruan L, Wei K, Wang L, Cheng H, Zhang F, Wu L, Bai P, Zhang C (2016) Characteristics of NH4+ and NO3− fluxes in tea (Camellia sinensis) roots measured by scanning ion-selective electrode technique. Sci Rep 6:38370. https://doi.org/10.1038/srep38370
Sage RF, Pearcy RW (1987) The nitrogen use efficiency of C3 and C4 plants: II. Leaf nitrogen effects on the gas exchange characteristics of Chenopodium album (L.) and Amaranthus retroflexus (L.). Plant Physiol 84(3):959–963. https://doi.org/10.1104/pp.84.3.959
Saha S, Monroe A, Day MR (2016) Growth, yield, plant quality and nutrition of basil (Ocimum basilicum L.) under soilless agricultural systems. Ann Agric Sci 61:181–186. https://doi.org/10.1016/j.aoas.2016.10.001
Shahrokhnia MH, Sepaskhah AR (2018) Water and nitrate dynamics in safflower field lysimeters under different irrigation strategies, planting methods, and nitrogen fertilization and application of HYDRUS-1D model. Environ Sci Pollut Res 25(9):8563–8580. https://doi.org/10.1007/s11356-017-1184-7
Sheehy JE, Dionora MJA, Mitchell PL, Peng S, Cassman KG, Lemaire G, Williams RL (1998) Critical nitrogen concentrations: implications for high-yielding rice (Oryza sativa L.) cultivars in the tropics. Field Crops Res 59:31–41
Singh K, Chand S, Yaseen M (2014) Integrated nutrient management in Indian basil (Ocimum basilicum). Ind Crop Prod 55:225–229. https://doi.org/10.1016/j.indcrop.2014.02.009
Taiz L, Zeiger E (2010) Plant physiology, 5th edn. Sinauer Associates Inc., Sunderland, pp 67–86
Tei F, Benincasa P, Guiducci M (2002) Critical nitrogen concentration in processing tomato. Eur J Agron 18:45–55
Tei F, Benincasa P, Guiducci M (2003) Critical nitrogen concentration in lettuce. Acta Hortic 627:187–194. https://doi.org/10.17660/ActaHortic.2003.627.24
Ulrich A (1952) Physiological bases for assessing the nutritional requirements of plants. Annu Rev Plant Physiol 3:207–228
Umar S, Anjana Anjum NA, Khan NA (2013) Nitrate management approaches in leafy vegetables. In: Umar S, Anjum NA, Khan NA (eds) Nitrate in leafy vegetables: toxicity and safety measures. IK International Publishing House Pvt. Ltd, New Delhi, pp 166–181
Wang Y, Wang D, Shi P, Omasa K (2014) Estimating rice chlorophyll content and leaf nitrogen concentration with a digital still color camera under natural light. Plant Methods 10:1. https://doi.org/10.1186/1746-4811-10-36
Wang Y, Shi P, Ji R, Min J, Shi W, Wang D (2019) Development of a model using the nitrogen nutrition index to estimate in-season rice nitrogen requirement. Field Crop Res 245:107664
Wood CW, Reeves DW, Himelrick DG (1993) Relationships between chlorophyll meter readings and leaf chlorophyll concentration, N status, and crop yield: a review. Proc Agron Soc 23:1–9
Xu HL, Wang R, Xu RY, Mridha MAU, Goyal S (2005) Yield and quality of leafy vegetables grown with organic fertilizations. Acta Hortic 627:25–33
Yan X, Gong W (2010) The role of chemical and organic fertilizers on yield, yield variability and carbon sequestration— results of a 19-year experiment. Plant Soil 331(1–2):471–480. https://doi.org/10.1007/s11104-009-0268-7
Yang H, Yang J, Lv Y, He J (2014) SPAD values and nitrogen nutrition index for the evaluation of rice nitrogen status. Plant Prod Sci 17(1):81–92. https://doi.org/10.1626/pps.17.81
Yuan MW, Couture JJ, Townsend PA, Ruark MD, Bland WL (2016) Spectroscopic determination of leaf nitrogen concentration and mass per area in sweet corn and snap bean. Agron J 108:2519–2526
Yue SC, Meng QF, Li F, Cui ZL, Zhang F, Chen XP, Zhao RF (2012) Critical nitrogen dilution curve for optimizing nitrogen management of winter wheat production in the North China plain. Agron J 104:523–529
Yue SC, Sun FL, Meng QF, Zhao RF, Li F, Chen XP, Zhang FS, Cui ZL (2014) Validation of a critical nitrogen curve for summer maize in the North China Plain. Pedosphere 24(1):76–83
Zha B, Duan A, Ata-Ul-Karim S, Liu Z, Chen Z, Gong Z et al (2018) Exploring new spectral bands and vegetation indices for estimating nitrogen nutrition index of summer maize. Eur J Agron 93:113–125. https://doi.org/10.1016/j.eja.2017.12.006
Zhao B (2014) Determining of a critical dilution curve for plant nitrogen concentration in winter barley. Field Crop Res 160:64–72. https://doi.org/10.1016/j.fcr.2014.02.016
Zhao B, Ata-Ul-Karim ST, Liu Z, Xiao J, Liu Z, Qin A, Ning D, Nan J, Duan A (2017) Development of a critical nitrogen dilution curve based on leaf dry matter for summer maize. Field Crops Res 208:60–68
Zhao B, Ata-Ul-Karim ST, Duan A, Liu Z, Wang X, Xiao J, Qin A, Ning D, Zhang W, Lian Y (2018) Determination of critical nitrogen concentration and dilution curve based on leaf area index for summer maize. Field Crop Res 228:195–203. https://doi.org/10.1016/j.fcr.2018.09.005
Ziadi N, Brassard M, Bélanger G, Cambouris AN, Tremblay N, Nolin MC et al (2008a) Critical nitrogen curve and nitrogen nutrition index for corn in eastern Canada. Agron J 100:271–276
Ziadi N, Brassard M, Bélanger G, Claessens A, Tremblay N, Cambouris AN et al (2008b) Chlorophyll measurements and nitrogen nutrition index for the evaluation of corn nitrogen status. Agron J 100:1264–1272. https://doi.org/10.2134/agrojnl2007.0059
Ziadi N, Bélanger G, Claessens A, Lefebvre L, Cambouris AN, Tremblay N, Nolin MC, Parent LÉ (2010) Determination of a critical nitrogen dilution curve for spring wheat. Agron J 102:241–250
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This study was funded by Iran National Science Foundation (INSF) (grant number 96013073).
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Rahimikhoob, H., Sohrabi, T. & Delshad, M. Development of a Critical Nitrogen Dilution Curve for Basil (Ocimum basilicum L.) Under Greenhouse Conditions. J Soil Sci Plant Nutr 20, 881–891 (2020). https://doi.org/10.1007/s42729-020-00174-5
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DOI: https://doi.org/10.1007/s42729-020-00174-5