Abstract
Nitrosomonas europaea is the most extensively studied ammonia-oxidizing bacterium (AOB), being a ubiquitous player in the conversion of ammonia in the soil environment. The precipitation of constituents of the standard culture medium, American Type Culture Collection (ATCC) #2265, further hinders both the study of N. europaea vital role in the nitrogen cycle and the development of biotechnological applications, as the presence of inorganic debris severely compromises scale-up procedures and downstream processing. The standard formulation was analyzed; the precipitate was identified as being struvite. Struvite is spontaneously formed when magnesium, phosphate, and ammonium are available in a high pH solution. This combination is common and transversal to a series of AOB media formulations. Therefore, a non-precipitating medium was developed. The modified medium does not require filter sterilization, which reduces 99% of its production cost. The kinetic performance of N. europaea was evaluated in the standard and modified medium. A precise and direct quantification of the bacterium cell growth profile was achieved using fluorescent in situ hybridization (FISH) and flow cytometry, which was correlated with other metabolic parameters. The performance of N. europaea was not impaired in the modified medium.
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References
Alfaro M, Salazar F, Hube S, Ramírez L, Mora MS (2018) Ammonia and nitrous oxide emissions as affected by nitrification and urease inhibitors. J Soil Sci Plant Nutr 18:479–486
Amoo AE, Babalola OO (2017) Ammonia-oxidizing microorganisms: key players in the promotion of plant growth. J Soil Sci Plant Nutr 17:935–947
Aoyagi R, Sato R, Terada A, Tokuyama H (2018) Novel composite gel beads for the immobilization of ammonia-oxidizing bacteria: fabrication, characterization, and biokinetic analysis. Chem Eng J 342:260–265
Ariyanto E, Sen TK, Ang HM (2014) The influence of various physico-chemical process parameters on kinetics and growth mechanism of struvite crystallisation. Adv Powder Technol 25:682–694
Austin D, Vazquez-Burney R, Dyke G, King T (2019) Nitrification and total nitrogen removal in a super-oxygenated wetland. Sci Total Environ 652:307–313
Barbosa SG, Peixoto L, Meulman B, Alves MM, Pereira MA (2016) A design of experiments to assess phosphorous removal and crystal properties in struvite precipitation of source separated urine using different Mg sources. Chem Eng J 298:146–153
Camargo JA, Alonso Á (2006) Ecological and toxicological effects of inorganic nitrogen pollution in aquatic ecosystems: a global assessment. Environ Int 32:831–849
Coskuner G, Ballinger SJ, Davenport RJ, Pickering RL, Solera R, Head IM, Curtis TP (2005) Agreement between theory and measurement in quantification of ammonia-oxidizing bacteria. Appl Environ Microbiol 71:6325–6334
Cruvellier N, Poughon L, Creuly C, Dussap C-G, Lasseur C (2016) Growth modelling of Nitrosomonas europaea ATCC® 19718 and Nitrobacter winogradskyi ATCC® 25391: a new online indicator of the partial nitrification. Bioresour Technol 220:369–377
Drozd JW (2018) Respiration in the ammonia-oxidizing chemoautotrophic bacteria. In: Kowles CJ (ed) Diversity of bacterial respiratory systems, Volume 1. CRC Press, Boca Raton, pp 87–112
Gao X, Wen Y, Qu D, An L, Luan S, Jiang W, Zong X, Liu X, Sun Z (2018) Interference effect of alcohol on Nessler’s reagent in photocatalytic nitrogen fixation. ACS Sustain Chem Eng 6:5342–5348
Hommes NG, Sayavedra-Soto LA, Arp DJ (2003) Chemolithoorganotrophic growth of Nitrosomonas europaea on fructose. J Bacteriol 185:6809–6814
Kim S, Patel DS, Park S, Slusky J, Klauda JB, Widmalm G, Im W (2016) Bilayer properties of lipid a from various gram-negative bacteria (2016). Biophys J 111:1750–1760
Kitjaruwankul S, Wapeesittipan P, Boonamnaj P, Sompornpisut P (2016) Inner and outer coordination shells of Mg2+ in CorA selectivity filter from molecular dynamics simulations. J Phys Chem B 120:406–417
Manser R, Muche K, Gujer W, Siegrist H (2005) A rapid method to quantify nitrifiers in activated sludge. Water Res 39:1585–1593
Mobarry BK, Wagner M, Urbain V, Rittmann BE, Stahl DA (1996) Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria. Appl Environ Microbiol 62:2156–2162
Oliveira JV, Alves MM, Costa JC (2015) Optimization of biogas production from Sargassum sp. using a design of experiments to assess the co-digestion with glycerol and waste frying oil. Bioresour Technol 175:480–485
Pan K-L, Gao J-F, Li H-Y, Fan X-Y, Li D-C, Jiang H (2018) Ammonia-oxidizing bacteria dominate ammonia oxidation in a full-scale wastewater treatment plant revealed by DNA-based stable isotope probing. Bioresour Technol 256:152–159
Papp B, Török T, Sándor E, Fekete E, Flipphi M, Karaffa L (2016) High cell density cultivation of the chemolithoautotrophic bacterium Nitrosomonas europaea. Folia Microbiol 61:191–198
Stein LY (2019) Insights into the physiology of ammonia-oxidizing microorganisms. Curr Opin Chem Biol 49:9–15
Funding
This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Susana Cortez would like to acknowledge the research grant funded by FCT (SFRH / BPD / 73720 / 2010). Gonzalo Tortella would like to acknowledge the FONDECYT project 1161713.
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Padrão, J., Tortella, G., Cortez, S. et al. Nitrifying Soil Bacterium Nitrosomonas europaea: Operational Improvement of Standard Culture Medium. J Soil Sci Plant Nutr 19, 270–276 (2019). https://doi.org/10.1007/s42729-019-00023-0
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DOI: https://doi.org/10.1007/s42729-019-00023-0