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Molecular characterization of Haplothrips cerealis Priesner 1939 as a synonym of Haplothrips tritici (Kurdjumov, 1912)

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Abstract

The present study was conducted utilizing collected thrips individuals from 39 localities in cereal production areas of Lakes of Region, Turkey during 2016–2017. Thrips individuals were sampled from plant foliage and ears using the destructive sample method (randomly in each field) and strike method (in each field, 100 beats). Specimens were transferred with a fine brush to 1.5 ml eppendorf tubes containing 95% ethanol and stored at -20 °C until analysis. Specimens were retrieved after the first step of DNA extraction and mounted onto slides using Canada balsam. Mitochondrial DNA Cytochrome Oxidase I gene location is used in the diagnosis of thrips. Polymerase Chain Reaction was performed using universal primers. 629–641 bp was obtained in the Cytochrome Oxidase I gene region of 59 individuals, including Haplothrips cerealis and Haplothrips tritici after morphological identification. Molecular characterization revealed that all the sequenced specimens were Haplothrips tritici.

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Acknowledgements

We thank the Scientific Research Projects Coordination Unit of Süleyman Demirel University, Turkey (project number 4642-D2-16) for the present research.Thanks to Hilal Şule TOSUN (Ph.D.) (University of Akdeniz, Faculty of Agriculture, Department of Plant Protection, Türkiye) for contributing to the article.

Funding

The study funded by the Scientific Research Projects Coordination Unit of Süleyman Demirel University, Turkey (project number 4642-D2-16). Not applicable.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, Isparta University of Applied Sciences, Isparta, 32260, Türkiye

    Asiye Uzun Yiğit & Ozan Demirözer

  2. Department of Agricultural Biotechnology, Faculty of Agriculture, Eskişehir Osmangazi University, Eskişehir, 26160, Türkiye

    Coşkun Güçlü

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  1. Asiye Uzun Yiğit
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  2. Ozan Demirözer
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  3. Coşkun Güçlü
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OD and AUY conceived the research, AUY performed the fieldwork and labwork, and CG performed data analysis. All authors wrote, read, and approved the manuscript.

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Correspondence to Asiye Uzun Yiğit.

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Uzun Yiğit, A., Demirözer, O. & Güçlü, C. Molecular characterization of Haplothrips cerealis Priesner 1939 as a synonym of Haplothrips tritici (Kurdjumov, 1912). Int J Trop Insect Sci (2024). https://doi.org/10.1007/s42690-024-01239-z

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