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The level of arylphorin in the haemolymph of Spodoptera mauritia Boisd. (Lepidoptera: Noctuidae) larvae is increased by the insecticide, pyriproxyfen

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Abstract

Rice swarming caterpillar, Spodoptera mauritia, is a major pest of paddy. Insect growth regulators are a class of substances that interfere with insects’ natural growth, development, and reproduction by disrupting physiological processes. Insect growth regulators are appropriate for pest management as they are more target-specific, non-persistent, biodegradable, and ecologically benign compounds with reduced toxicity to non-target organisms. In this study, we showed that treatment of 5th instar day 0 larvae of S. mauritia with the JH-mimic, pyriproxyfen, increases the level of an 83 kDa haemolymph protein band in SDS-PAGE compared to control. The pyriproxyfen–responsive protein was identified by mass spectrometry as arylphorin, a hexameric storage protein. It is revealed that in native state it is a high molecular weight multimeric protein. Also we showed that the increase in level of arylphorin on exposure to pyriproxyfen is the result of induction of protein synthesis. As storage proteins are crucial for insect development and metamorphosis and the level is altered by insecticides like pyriproxyfen, the role of arylphorin in the physiology of the larvae exposed to insecticides needs to be explored.

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Acknowledgements

University Grants Commission, New Delhi for financial support, UGC Special Assistance Programme (SAP), Department of Zoology and Centre for Advances in Molecular Biology (CAMB), University of Calicut for the facilities.

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This work was supported by the University Grants Commission, New Delhi.

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  1. Department of Zoology, University of Calicut, Thenjipalam (PO), Malappuram, Kerala, India

    Resmitha C & Kannan Vadakkadath Meethal

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Correspondence to Kannan Vadakkadath Meethal.

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C, R., Meethal, K.V. The level of arylphorin in the haemolymph of Spodoptera mauritia Boisd. (Lepidoptera: Noctuidae) larvae is increased by the insecticide, pyriproxyfen. Int J Trop Insect Sci 43, 655–664 (2023). https://doi.org/10.1007/s42690-023-00975-y

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