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Transcriptome analysis of wolf spider Pardosa laura exposed to beta-cypermethrin

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Abstract

To understand the molecular mechanism of Pardosa laura in response to beta-cypermethrin (beta-CP) stress, next generation RNA-seq technique was employed to analyse the expression patterns of resistance- and stress-response genes in P. laura subjected to beta-CP. De novo sequence assembly yielded 49,003 unigenes, 20,912 of which were annotated in the public databases. A total of 370 up or down-regulated genes altered by beta-CP were detected, among them, 318 unigenes were upregulated, including one GST, one GPx, five CYP450s, two HSPs (one HSP70 and one HSP20) and two ABC transporters. Functional enrichment analysis revealed that carbon metabolism, glutathione metabolism, drug metabolism-CYP450, oxidative phosphorylation, as well as lysosome, phagosome and mTOR signaling pathway were predominately enriched in response to beta-CP stress. Certain critical genes involved in detoxification were detected to be differentially expressed between the beta-CP treated and control spiders. These results provide a fundamental resource for further research in the molecular characterisation of P. laura responding to pesticide.

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Data accessibility

Transcriptome sequencing raw data are available in the NCBI Short Archive (SRA) database with accession number PRJNA735032, including the transcriptome TC1 (SRR14728264), TC2 (SRR14728263), TC3 (SRR14728262), T-CBP1 (SRR14728261), T-CBP2 (SRR14728260) and T-CBP3 (SRR14728259).

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Funding

This study was supported by Science and Technology Innovation Program of Hubei Province (2018ABA098), Central-guided Local Project of Sci & Tech Development (2019ZYYD045), Natural Science Fund of Hubei Province (2017CFB609).

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Author notes

  1. Qing Ning and Jing Li these authors contributed equally to this work.

Authors and Affiliations

  1. Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Xiaogan, 432000, China

    Qing Ning, Jing Li, Li-hua Wang & Chang-chun Li

  2. Hubei Province Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Xiaogan, 432000, China

    Yu-jun Dai & Chang-chun Li

  3. College of Life Science and Technology, Hubei Engineering University, Xiaogan, 432000, China

    Qing Ning, Jing Li, Li-hua Wang, Yu-jun Dai & Chang-chun Li

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  1. Qing Ning
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  2. Jing Li
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  3. Li-hua Wang
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  4. Yu-jun Dai
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  5. Chang-chun Li
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Contributions

Chang-chun Li conceived and designed the experiments; Qing Ning and Jing Li performed the experiments; Chang-chun Li and Yu-Jun Dai analysed the output data; Chang-chun Li and Li-Hua Wang prepared the manuscript.

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Correspondence to Chang-chun Li.

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Materials used in this study were unregulated common arthropod spider and D. melanogaster.

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The authors declare no conflict of interest.

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Ning, Q., Li, J., Wang, Lh. et al. Transcriptome analysis of wolf spider Pardosa laura exposed to beta-cypermethrin. Int J Trop Insect Sci 42, 865–873 (2022). https://doi.org/10.1007/s42690-021-00611-7

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  • DOI: https://doi.org/10.1007/s42690-021-00611-7

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