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Comparative study of antifungal potential of various extracts of leaves of Carissa carandas Linn., Nerium oleander Linn. and Allamanda cathartica Linn. against human fungal pathogen Candida albicans

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Abstract

Candida albicans is the most significant species of opportunistic fungus that causes infections in immunocompromised people with invasive fungal illnesses. Scientists are now paying more attention to herbs used in traditional medicine as a result of Candida species’ resistance to antifungal medications. This study was conducted to compare the antifungal impacts of herbal leaf extracts of Carissa carandas Linn., Nerium oleander Linn., and Allamanda cathartica Linn. due to the limitations in the treatment of fungal diseases, like high prices, adverse side effects, drug resistance, or reduced susceptibility to fungal drugs. Leaves were collected, dried, and extracted using standard techniques for alkaloids, flavonoids, steroids, and crude extracts in petroleum ether, methanol, and water, then tested for their antifungal efficacy. A disc diffusion assay was used for screening, and the inhibition zone and activity index were calculated. The broth micro dilution method was used to calculate the minimum inhibitory concentration and the minimum fungicidal concentration. Total activity, mean, and standard deviation were also calculated. Alkaloid extracts of A. cathartica Linn. showed the best activity (IZ = 22 mm, AI = 0.650.01, MIC = 0.078, MFC = 0.156 mg/mL, TA = 557.69 mL). Results showed that among all the examined leaf extracts from the three selected plants, the alkaloid extract of A. cathartica Linn. leaves was the most efficient against Candida albicans. As a result, this plant may be investigated for the development of novel and improved herbal antifungal medications.

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References

  • Andrews JM (2001) BSAC standardized disc susceptibility testing method. J Antimicrob Chemother 4:43–57

    Google Scholar 

  • Bameta A, Kumari A, Upadhyaya A (2017) Phytochemical analysis and antimicrobial activity of Nerium oleander Linn. Phytochem Anal 2:2455–6548

    Google Scholar 

  • Basri DF, Fan SH (2005) The potential of aqueous and acetone extracts of gall of Quercusinfectoria as antibacterial agents. Indian J Pharmacol 37:26–29

    Article  Google Scholar 

  • Bongomin F, Gago S, Oladele RO, Denning DW (2017) Global and multi-national prevalence of fungal diseases-estimate precision. J Fungus 3(4):57

    Article  Google Scholar 

  • Brain KR, Turner TD (1975) The practical evaluation of phytopharmaceuticals. Wright-Scientica, Bristol, pp 57–58

    Google Scholar 

  • Chaudhary K, Prasad DN (2018) Antimicrobial studies on Nerium oleander Linn. leaves (White Kaner leaves). Int Res J Pharm 9(2):45–47

    Article  Google Scholar 

  • Dardona AWY, Shahabuddin D (2022) Evaluation of antimicrobial activity of methanolic and ethanolic extracts of three varieties of Nerium oleander. J Pharm Negat 13(7):1821–1829

    Google Scholar 

  • Demain AL, Sanchez S (2009) Microbial drug discovery: 80 years of progress. J Antibiot 62:5–16

    Article  CAS  Google Scholar 

  • Devanaboyina N, RamaLakshmi N, Satyanarayana B, Sudeepthi P, Hemachakradhar K, Pavankumar RN (2013) Preliminary phytochemical screening, quantitative estimation and evaluation of antimicrobial activity of Alstoniamacrophylla stem bark. Int J Sci Invent Today 2(1):31–39

    Google Scholar 

  • Dhatwalia J, Kumari A, Verma R et al (2021) Phytochemistry, pharmacology, and nutraceutical profile of carissa species: an updated review. Molecules. https://doi.org/10.3390/molecules26227010

    Article  PubMed  PubMed Central  Google Scholar 

  • Eloff JN (2004) Quantifying the bioactivity of the plant extracts during screening and bioassay-guided fractionation. Phytomedicine 11(4):370–371

    Article  CAS  PubMed  Google Scholar 

  • Evans WC (1996) ‘Trease & Evans’ pharmacognosy, 14th edn. WB Saunders Co., Philadelphia, p 573

    Google Scholar 

  • Fartyal M (2016a) Allamanda cathartica Linn.: extraction and pharmaceutical evaluation of various extracts of leaves and flowers. Int J Curr Pharm Res 8(4):28–32. https://doi.org/10.22159/ijcpr.2016v8i4.15272

    Article  CAS  Google Scholar 

  • Fartyal M (2016b) Antifungal sscreening of alkaloids, flavonoids & steroids of different parts of Nerium oleander Linn. Int J Acad Res 3,7(4):132–141

    Google Scholar 

  • Fartyal M, Kumar P (2014a) Bioactivity of crude extracts of Carissa carandas Linn. Linn. Linn. extracted in polar and non-polar solvents. Int J Eng SciInnov Technol 3(5):186

    Google Scholar 

  • Fartyal M, Kumar P (2014b) Bioactivity of crude extracts of Nerium oleander Linn. extracted in polar and non-polar solvents. J Sci Innov Res 3(4):426–432

    Article  Google Scholar 

  • Fartyal M, Kumar P (2016a) Antifungal efficacy of alkaloids, flavonoids and steroids of different parts of Carissa carandas Linn. Linn. Linn. Asian J Sci Technol 7(8):3353–3357

    CAS  Google Scholar 

  • Fartyal M, Kumar P (2016b) Pharmacological evaluation of crude extracts of Allamanda cathartica linn. extracted in polar and non-polar solvents. Int J Curr Res 8(8):35730–35735

    CAS  Google Scholar 

  • Gong Y, Liu W, Huang X, Hao L, Li Y, Sun S (2019) Antifungal activity and potential mechanism of N-butylphthalide alone and in combination with fluconazole against Candida albicans. Front Microbiol 10:1461. https://doi.org/10.3389/fmicb.2019.01461

    Article  PubMed  PubMed Central  Google Scholar 

  • Harborne JB (1973) Phytochemical methods. Chapman & Hall, Ltd., London, pp 49–188

    Google Scholar 

  • Hema K, Krishnaveni R (2014) Antibacterial and antifungal activities of Allamanda cathartica Linn. Int J Pharma Bio Sci 5:588–593

    Google Scholar 

  • Jia Z, Tang M, Wu J (1999) The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals. Food Chem 64(4):555–599

    Article  Google Scholar 

  • Massiha A, Muradov PZ (2015) Comparison of antifungal activity of extracts of ten plant species and griseofulvin against human pathogenic dermatophytes. Zahedan J Res Med Sci 17(10):e2096

    Article  Google Scholar 

  • Mehta R, Dhruv S, Kaushik V, Sen KK, Khan NS, Abhishek A, Dixit AK, Tripathi VN (2020) A comparative study of antibacterial and antifungal activities of extracts from four indigenous plants. Bioinformation 16(3):267–273. https://doi.org/10.6026/97320630016267

    Article  PubMed  PubMed Central  Google Scholar 

  • Nath MC, Chakravorty MK, Chowdhury SR (1946) Liebermann-Burchard reaction for steroids. Natur 157(3978):103–104

    Article  CAS  Google Scholar 

  • Petricevich VL, Abarca-Vargas R (2019) Allamanda cathartica: a review of the phytochemistry, pharmacology, toxicology, and biotechnology. Molecules 24:1238. https://doi.org/10.3390/molecules24071238

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Pilasombut K, Laosinwattana C, Tuyen Nguyen TK, Ngamyeeesoon N, Teerarak M (2019) Antimicrobial properties of extracts from Carissa carandas Linn. Linn. L. fruits and its application in chilled and frozen ground pork. Int J AgricTechnol 15(1):91–102

    CAS  Google Scholar 

  • Ramawat KG, Merillon JM (2000) Biotechnology: secondary metabolites. Science Pub Inc.

    Google Scholar 

  • Scio E, Mendes RF, Motta EVS, Bellozi PMQ (2012) Antimicrobial and antioxidant activities of some plant extract. Phytochemicals as Nutraceuticals-Global Approaches to their Role in Nutrition and Health, INTECH Europe, pp 21–42

  • Singh S, Bajpai M, Mishra P (2020) Carissa carandas Linn. Linn. L.—Phyto-pharmacological review. J Pharm Pharmacol 72:1694–1714

    Article  CAS  PubMed  Google Scholar 

  • Sofowara A (1993) Medicinal plants & traditional medicine in Africa. Spectrum Books Ltd, Ibadan, p 289

    Google Scholar 

  • Subramanian SS, Nagarjan S (1969) Flavonoids of the seeds of Crotolariaretusa and Crotolariastriata. Curr Sci 38:65

    CAS  Google Scholar 

  • Suleyman G, Alangaden GJ (2016) Nosocomial fungal infections: epidemiology, infection control, and prevention. Infect Dis Clin North Am 30:1023–1052. https://doi.org/10.1016/j.idc.2010.11.003

    Article  PubMed  Google Scholar 

  • Supaphon P, Preedanon S (2019) Antimicrobial and antioxidant activities of endophytic fungi extracts isolated from Carissa carandas Linn. Linn. Afr J Microbiol Res 13(27):464–473

    Article  CAS  Google Scholar 

  • Talapko J, Juzbašić M, Matijević T, PustijanacE BS, Kotris I, Škrlec I (2021) Candida albicans—the virulence factors and clinical manifestations of infection. J Fungus 7(2):79

    Article  CAS  Google Scholar 

  • Tomita Y, Uomori A, Minato H (1970) Steroidal sapogenins and sterols in tissue cultures of Dioscoreatokora. Phytochemistry 9:111–114

    Article  CAS  Google Scholar 

  • Verma S, Chaudhary HS (2011) Effect of Carissa carandas against clinically pathogenic bacterial strains. J Pharm Res 4:3769–3771

    CAS  Google Scholar 

  • Wankhede SB, Routh MM, Rajput SB, Karuppayil SM (2013) Antifungal properties of selected plants of Apocynaceae family against the human fungal pathogen Candida albicans. Int Curr Pharm J 2(7):122–125

    Article  Google Scholar 

Download references

Acknowledgements

The author is thankful to the Head of the Department of Botany, University of Rajasthan, for providing all necessary facilities and to Prof. Padma Kumar for providing language help, writing assistance, and proofreading the article.

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  1. Department of Biotechnology, Kanoria PG Mahila Mahavidhyalaya, Jaipur, Rajasthan, India

    Meenakshi Fartyal

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Correspondence to Meenakshi Fartyal.

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Fartyal, M. Comparative study of antifungal potential of various extracts of leaves of Carissa carandas Linn., Nerium oleander Linn. and Allamanda cathartica Linn. against human fungal pathogen Candida albicans. Vegetos (2023). https://doi.org/10.1007/s42535-023-00756-5

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