Abstract
A study was conducted to evaluate the in vitro antifungal activity of selected medicinal plant extracts against clinical isolates of Candida species, determine the phytochemicals present in various medicinal plant extracts with antifungal activity, and analyze the type of interaction exhibited by combinations of plant extracts and antifungal compound for the treatment of candidiasis. Thirty plant samples were extracted and used in the preparation of dichloromethane (DCM), methanol, decoction, and crude extracts. These extracts with and without combination of antibiotic were tested against three species of Candida (C. albicans, C. parapsilosis, C. krusei) using disk diffusion assay. The methods of Cannell (Methods in biochemistry: natural products isolation, Humana Press Inc., Totowa, 1998) and Roopashree et al. (Int J Appl Res Nat Prod 1(3):20–28, 2008) were adopted to identify the general phytochemical groups present in the plant extracts. Results showed that potential activities depend on the method of extraction and the solvents used. Five out of 30 DCM extracts inhibited C. albicans mildly, 13 showed antifungal activity against C. parapsilosis, and 25 extracts demonstrated growth inhibition of C. krusei. More plant samples using DCM as solvent inhibited C. krusei than C. parapsilosis and C. albicans. Qualitative phytochemical analyses revealed the presence of various compounds in the extract. Combinations of plant extracts and antibiotic resulted to different types of interaction: antagonism, additive, or synergism.
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Ilagan, Y.A., Ilagan, F.M.A., Ramos, A.P. (2015). Antifungal Activity of Some Medicinal Plants and Their Interactive Effects with Antibiotic on Some Species of Candida Causing Candidiasis. In: Gnanamalar Sarojini Daniel, E. (eds) Biology Education and Research in a Changing Planet. Springer, Singapore. https://doi.org/10.1007/978-981-287-524-2_18
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DOI: https://doi.org/10.1007/978-981-287-524-2_18
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