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Destructive mycolytic suppression of Fusarium oxysporum causing wilt in chickpea by fungicide tolerant actinobacteria

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Abstract

The present study is aimed to evaluate the integrated effects of the chemical fungicide Bavistin (carbendazim 50% WP) on adaptive variants of rhizospheric actinobacteria in providing protection to the chickpea from wilt caused by Fusarium oxsysporum. Actinobacterial isolates recovered from the rhizospheric region were screened for their antibiosis against the fungal pathogen F. oxysporum. Among these actinobacteria two potent antagonistic isolates, Nocardiopsis sp. KWC01 and Streptomyces sp. KBR01, showed a significant profile by producing extracellular lytic enzymes, hydrogen cyanide, siderophore, indole acetic acid (IAA) and solubilizing phosphate. Both of them caused hyphal deformation in F. oxysporum as observed through scanning electron microscopy (SEM). Before applying a blended form of biological agents and the chemical in the field, bavistin adaptive variants of both the actinobacterial isolates were obtained. Thereafter EC50 of bavistin to kill F. oxysporum was determined and applied with actinobacterial isolates during field trials. The effect of EC50 of Bavistin on the specific growth rate of isolates was also examined, which showed enhanced growth of isolates at concentrations close to EC50 of bavistin. During field trials, unsurpassed results were obtained using blends of the actinobacterial consortium with a low dose of chemical fungicide. This combination led to an increase in wilt protection by 2.66% and grain yield by 8.69% over full dose of chemical fungicide. These results advocate the efficiency of integrated formulation containing Nocardiopsis sp. KWC01, Streptomyces sp. KBR01 and low dose of bavistin in wilt management and productivity enhancement of chickpea plants.

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Acknowledgements

The authors are grateful to the Department of Botany and Microbiology, Gurukula Kangri University, Haridwar for providing essential services and conveniences to carry out this research.

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Authors and Affiliations

  1. Department of Microbiology, Kanya Gurukula Campus, Gurukula Kangri Vishwavidyalaya, Haridwar, Uttarakhand, India

    Trepti Singh & Verinder Wahla

  2. Department of Botany and Microbiology, Gurukula Kangari Vishwavidyalaya, Haridwar, Uttarakhand, India

    R. C. Dubey & D. K. Maheshwari

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  1. Trepti Singh
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  2. Verinder Wahla
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  3. R. C. Dubey
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  4. D. K. Maheshwari
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Correspondence to Trepti Singh.

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Singh, T., Wahla, V., Dubey, R.C. et al. Destructive mycolytic suppression of Fusarium oxysporum causing wilt in chickpea by fungicide tolerant actinobacteria. Environmental Sustainability 5, 243–253 (2022). https://doi.org/10.1007/s42398-022-00229-6

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