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Jurinea humilis DC. Polar Extract: HPLC Analysis, Photoprotective, Antioxidant Activities and Bioactive Content

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Abstract

The use of botanical antioxidants to protect human skin from the harmful effects of ultraviolet radiation (UVR) opens the door to new and exciting avenues of research in photoprotection. Besides, for the sake of long-term sustainability, this strategy is both environmentally and human-friendly. To that end, the current study focuses on the polar extract (n-butanol) of Jurinea humilis DC. Several spectrophotometric methods were used to determine the total bioactive content of this extract. HPLC analysis was also used to determine the chemical profile of Jurinea humilis n-butanol extract. In addition, the antioxidant capacity of the sample was determined using the phosphomolybdenum method, as well as DPPH and H2O2 scavenging activities. The sun protection factor (SPF) was calculated using Mansur's dilution method, to assess photoprotective activity. The results showed that polar extract contained a high level of polyphenols, specifically phenolic content (171.18 ± 1.06 mg GAE/g extract), flavonol content (128.62 ± 4.42 mg RE/g extract), and flavonoid content (109.46 ± 0.25 mg QE/g extract), as well as potential antioxidant activity using phosphomolybdenum method (199.72 ± 3.71 mg AAE/g extract), DPPH inhibition (> 90%) and H2O2 scavenging activity (> 70%). Furthermore, at a concentration of 2 mg/mL, it has higher photoprotective properties, with a maximum SPF of 36.22 ± 0.07. Several polyphenols were discovered for the first time in the active extract using HPLC analysis. Therefore, Jurinea humilis polar extract appears to be a promising cosmetic ingredient.

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Acknowledgements

This study was supported by Ministry of Higher Education and Scientific Research of Algeria (Grant no. PRFU N° B00L01UN180120220002).

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Authors and Affiliations

  1. Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit, Department of Chemistry, University Mentouri Constantine 1, 25000, Constantine, Algeria

    Radia Ayad, Mostefa Lefahal, Nabila Zaabat, El Hani Makhloufi, Warda Trifa, Salah Akkal & Kamel Medjroubi

  2. Laboratory of Phytochemistry and Pharmacology, Department of Chemistry, Science Exact Faculty, University of Jijel, BP. 98, Ouled Aissa, 18000, Jijel, Algeria

    Radia Ayad

  3. Department of Biology, Faculty of Science, Selcuk University, Konya, Turkey

    Hatice Banu Keskinkaya

  4. Department of Nutrition and Dietetics, Faculty of Health Sciences, Igdir University, Igdir, Turkey

    Mehmet Nuri Atalar & Ibrahim Demirtas

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Contributions

RA: conceptualization, methodology, investigation, writing—original drafts. HBK: resources, data curation, formal analysis, validation. MNA: resources, data curation, formal analysis, validation. ML: methodology, validation. NZ: methodology, validation. EHM and WT: software, validation. ID: resources, data curation, formal analysis, validation. SA and KM: resources, validation, supervision.

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Correspondence to Radia Ayad.

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Ayad, R., Keskinkaya, H.B., Atalar, M.N. et al. Jurinea humilis DC. Polar Extract: HPLC Analysis, Photoprotective, Antioxidant Activities and Bioactive Content. Chemistry Africa 6, 827–836 (2023). https://doi.org/10.1007/s42250-022-00525-y

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