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Evaluation of the cytotoxic effects of silver-zinc oxide nanoparticles synthesized by green method on sw480 cell line

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Abstract

In recent years, metal nanocomposites (NCs) have widely been studied due to their several applications in therapeutic activities. The current study synthesized silver and zinc oxide nanocomposite (Ag/Zno NCs) by the green method using Artemisia tschernieviana ethanol extract and investigated the physical and chemical properties of Ag/Zno NCs by several instruments. Artemisia tschernieviana extract acted as a reducing/capping agent. Microscopic results revealed that the NCs were agglomerated, mostly spherical in shape, and having an average diameter of 50 nm. The in vitro cytotoxicity of Ag/Zno NCs was evaluated by MTT assay toward human colon cancer cells (SW480) and normal cells (HEK-293–293), and showed dosage-dependent inhibition activity of Ag/Zno NCs. Besides, the MTT results showed that NCs had lower IC50 against SW480 cells compared to the HEK-293–293 cell line. After treatment, the expressions of P53, Caspase-3, Bax, and Bcl-2 in SW480 cells were investigated by quantitative real-time PCR method. Bax, Bcl-2, and caspase-3 genes were highly expressed compared to the control group. In addition, Bax to Bcl-2 proportion was significantly increased in SW480 treated cells and revealed that Ag/Zno NCs can induce apoptosis in this cell line. In conclusion, this study highlighted the anticancer properties of green synthesized Ag/ZnO NCs using Artemisia tschernieviana, suggesting that these NCs had anti-proliferative potential against human colon cancer cell lines.

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Data availability

The datasets used during the current study are included in this article and additional information are available from the corresponding author on reasonable request.

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Author notes

  1. Rayehe Helalat and Niloufar Rezaei contributed equally to this work.

Authors and Affiliations

  1. Department of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

    Rayehe Helalat & Fahimeh Baghbani-Arani

  2. Department of Biology, School of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

    Mahdavi-Ourtakand Masoumeh

  3. Department of Regenerative Medicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, 16635148, Iran

    Niloufar Rezaei

Authors
  1. Rayehe Helalat
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  2. Mahdavi-Ourtakand Masoumeh
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  3. Niloufar Rezaei
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Contributions

F. B. designed and supervised the study. R. H. performed the experiments and M. M. O. and N. R. participated in analysis, interpretation of data, and paper writing. All authors read and approved the final manuscript.

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Correspondence to Fahimeh Baghbani-Arani.

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Helalat, R., Masoumeh, MO., Rezaei, N. et al. Evaluation of the cytotoxic effects of silver-zinc oxide nanoparticles synthesized by green method on sw480 cell line. emergent mater. 6, 291–298 (2023). https://doi.org/10.1007/s42247-022-00413-8

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  • DOI: https://doi.org/10.1007/s42247-022-00413-8

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