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A bifunctional bone scaffold combines osteogenesis and antibacterial activity via in situ grown hydroxyapatite and silver nanoparticles

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Abstract

Hydroxyapatite (HA) nanoparticles and silver (Ag) nanoparticles are expected to enable desirable bioactivity and antibacterial properties on biopolymer scaffolds. Nevertheless, interfacial adhesion between HA/Ag and the biopolymer is poor due to the large physicochemical differences between these components. In this study, poly L-lactic acid (PLLA) powder was first surface-modified with bioactive polydopamine (PDA) in an alkaline environment. Next, HA and Ag nanoparticles were grown in situ on the PDA-coated PLLA powder, which was then adhered to the porous bone scaffold using a selective laser-sintering process. Results showed that HA and Ag nanoparticles were homogenously distributed in the matrix, with enhanced mechanical properties. Simulated body fluid bioactivity tests showed that the in situ grown HA-endowed scaffold shows excellent bioactivity. In vitro tests confirmed that the scaffold exhibits favorable biocompatibility with human umbilical cord mesenchymal stem cells, as well as strong antibacterial activity against Gram-negative Escherichia coli. Furthermore, in vivo assays indicated that the scaffold promoted bone generation, with a new bone area fraction of 71.8% after 8 weeks’ implantation, without inflammation.

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Acknowledgements

This study was supported by the following funds: (1) National Natural Science Foundation of China (Nos. 51935014, 82072084, and 81871498); (2) Jiangxi Provincial Natural Science Foundation of China (Nos. 20192ACB20005 and 2020ACB214004); (3) The Provincial Key R & D Projects of Jiangxi (No. 20201BBE51012); (4) Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2018); (5) Shenzhen Science and Technology Plan Project (No. JCYJ20170817112445033); (6) Innovation Team Project on University of Guangdong Province (No. 2018GKCXTD001); (7) Technology Innovation Platform Project of Shenzhen Institute of Information Technology 2020 (No. PT2020E002); (8) Open Research Fund of Jiangsu Key Laboratory of Precision and Micro-Manufacturing Technology; (9) China Postdoctoral Science Foundation (No. 2020M682114).

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Authors and Affiliations

  1. Institute of Bioadditive Manufacturing, Jiangxi University of Science and Technology, Nanchang, 330013, China

    Youwen Yang, Yun Cheng, Fang Deng & Cijun Shuai

  2. Jiangsu Key Laboratory of Precision and Micro-Manufacturing Technology, Nanjing University of Aeronautics and Astronautics, Nanjing, 210016, China

    Youwen Yang & Lida Shen

  3. Shenzhen Institute of Information Technology, Shenzhen, 518172, China

    Zhenyu Zhao & Cijun Shuai

  4. NHC Key Laboratory of Carcinogenesis, School of Basic Medical Science, Central South University, Changsha, 410013, China

    Shuping Peng

  5. School of Energy and Machinery Engineering, Jiangxi University of Science and Technology, Nanchang, 330013, China

    Shuping Peng

  6. State Key Laboratory of High Performance Complex Manufacturing, Central South University, Changsha, 410083, China

    Cijun Shuai

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  1. Youwen Yang
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  2. Yun Cheng
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  3. Fang Deng
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  4. Lida Shen
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  5. Zhenyu Zhao
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Contributions

YWY and YC were involved in conceptualization, investigation, writing—original draft; FD and LDS were involved in visualization and resources; ZYZ and SPP helped in writing—review & editing; and CJS contributed to supervision.

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Correspondence to Shuping Peng or Cijun Shuai.

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The authors declare that they have no conflict of interest.

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The animal experiments in this work have obtained the permission from Xiangya Hospital Animal Experimental Ethics Committee.

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Yang, Y., Cheng, Y., Deng, F. et al. A bifunctional bone scaffold combines osteogenesis and antibacterial activity via in situ grown hydroxyapatite and silver nanoparticles. Bio-des. Manuf. 4, 452–468 (2021). https://doi.org/10.1007/s42242-021-00130-x

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