Abstract
The advent of SHG microscopy for biological tissue characterization has opened up avenues in probing ultrastructural features of the sample. The nonlinear polarization properties of collagen manifest in the tissue SHG polarimetry response. As compared to the anisotropic properties of collagen probed using linear polarimetry, SHG probes into the alterations to tropocollagen structures and thus aids in representing early alterations during pathological tissue transformation. This review summarizes SHG polarimetric studies with stromal collagen and correlations with histopathology, primarily for cancerous tissues. Various configurations, such as basic SHG polarization microscopy, polarization in polarization out, and Stokes–Mueller, are discussed with relevant clinical applications.
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Shaji, C., Sharma, M. & Unni, S.N. Optical polarization response of collagen—role in clinical cancer diagnostics: part 2. ISSS J Micro Smart Syst 11, 43–59 (2022). https://doi.org/10.1007/s41683-022-00102-y
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DOI: https://doi.org/10.1007/s41683-022-00102-y