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Copper Nitroprusside-Based Electrochemical Sensor for Detection of Tryptophan

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Abstract

This work presents a novel electrochemical approach for detecting tryptophan through its interaction with copper nitroprusside, which is synthesized using a simple chemical co-precipitation method. The utilization of the reduction reaction inherent to copper nitroprusside effectively exhibits high selectivity against common interferences present in urine, such as melatonin, lactate, cytosine, cytidine, urea, ascorbic acid, creatine, creatinine, tyrosine, glycine, alanine, arginine, and lysine. The method demonstrates two linear ranges: 0.0–0.15 mmol/L and 0.15–2.0 mmol/L with the sensitivities of 119.7 ± 0.2 µA/(mmol/L) and 9.9 ± 0.4 µA/(mmol/L), respectively. The limit of detection (3SB/m) was determined to be 5.5 µmol/L. Application of the sensor in synthetic urine yielded the recovery of 103% ± 5%.

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Data availability

The authors declare that the data supporting the findings of this study are available within the paper. Should any raw data files be needed in another format they are available from the corresponding author upon reasonable request.

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Acknowledgements

This research has received funding support from (i) Suranaree University of Technology (SUT) and (ii) the NSRF via the Program Management Unit for Human Resources & Institutional Development, Research and Innovation (PMU-B) (B13F660067). Ngamchuea K. acknowledges funding from (i) Suranaree University of Technology (SUT), (ii) Thailand Science Research and Innovation (TSRI), and (iii) National Science, Research and Innovation Fund (NSRF, NRIIS number 189603).

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Authors and Affiliations

  1. School of Chemistry, Institute of Science, Suranaree University of Technology, 111 University Avenue, Suranaree, Muang, Nakhon Ratchasima, 30000, Thailand

    Pachanuporn Sunon, Tidapa Rattanaumpa, Kornkanok Phakhunthod, Keerakit Kaewket, Pantipa Sawatmuenwai, Theeranun Siritanon & Kamonwad Ngamchuea

  2. Institute of Research and Development, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand

    Pachanuporn Sunon

  3. Synchrotron Light Research Institute, Nakhon Ratchasima, 30000, Thailand

    Suttipong Wannapaiboon

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  1. Pachanuporn Sunon
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Correspondence to Kamonwad Ngamchuea.

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Sunon, P., Rattanaumpa, T., Phakhunthod, K. et al. Copper Nitroprusside-Based Electrochemical Sensor for Detection of Tryptophan. J. Anal. Test. 7, 405–415 (2023). https://doi.org/10.1007/s41664-023-00280-0

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