Abstract
Elicitors are considered sustainable alternatives for the management of phytopathogenic viruses. The aim of the present study was to evaluate the effectiveness of nine elicitors on morphology, yield, and fruit quality of tomato plants inoculated with the Tomato brown rugose fruit virus (ToBRFV-Tobamovirus/Virgaviridae), Pepino mosaic virus (PepMV-Potexvirus/Alphaflexiviridae), and mixed infection (ToBRFV + PepMV). The experiment was set up under a completely randomized design with six replicates. Ten days after transplanting, virus inoculation was done mechanically in all the treatments, except the negative controls. Three morphological, four yield, and seven fruit quality variables were evaluated. Virablock® 3G50 increased yield by more than 44% compared with the positive control in plants inoculated with ToBRFV, PepMV, and the mixed infection, while Supermagro cell extract and enhanced Supermagro increased yield in plants inoculated with ToBRFV and the mixed infection by more than 82% and 34%, respectively. Virablock® 3G50 and an ethanolic extract of Arracacia bracteata showed the highest values in fruit quality variables.
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Ahmed SA, Baig MMV (2014) Biotic elicitor enhanced production of psoralen in suspension cultures of Psoralea corylifolia L. Saudi J Biol Sci 21(5):499–504. https://doi.org/10.1016/j.sjbs.2013.12.008
Benítez-Benítez R, Sarria-Villa RA, Gallo-Corredor JA, Pérez PNO, Álvarez SJH, Giraldo ACI (2019) Obtaining and yield of ethanolic extract of two medicinal plants. Revista Facultad De Ciencias Básicas 15(1):31–40. https://doi.org/10.18359/rfcb.3597
Elhelaly SH, El-shennawy MZ (2022) Different detection methods of Tomato mosaic virus (ToMV) and inducing resistance on bell pepper by some plant extracts. Menoufia J Plant Prot 7:41–51. https://doi.org/10.21608/MJAPAM.2022.228662
EPPO (2020) Tomato brown rugose fruit virus. OEPP/EPPO Bull 50:529–534. https://doi.org/10.1111/epp.12711
Gergerich RC, Dolja VV (2006) Introduction to Plant viruses, the invisible foe. Plant Health Instruct. https://doi.org/10.1094/PHI-I-2006-0414-01
GreenCorp (2022) Virablock® 3G50. https://greencorp.mx/producto/biocontrol/bioinductores-de-resistencia/virablock-3g50/#:~:text=Virablock%C2%AE%203G50%20is%C3%A1%20targeted,with%20virus%20but%20without%20s%20s%C3%ADntomas. Accessed 19 May 2023
Hanssen IM, Thomma BP (2010) Cucumber mosaic virus: A successful pathogen that rapidly evolved from emerging to endemic in tomato crops. Mol Plant Pathol 11(2):179–189. https://doi.org/10.1111/j.1364-3703.2009.00600.x
Hernández-Santiago R, Vargas-Hernández M, Zamora-Macorra EJ (2020) Evaluation of TMV resistance inducers in tomato. Remexca 11:377–390. https://doi.org/10.29312/remexca.v11i2.2072
Hou W, Li S, Massart S (2020) Is there a “Biological desert” with the discovery of new plant viruses? A retrospective analysis for new fruit tree viruses. Front Microbiol 11:592816. https://doi.org/10.3389/fmicb.2020.592816
Iobbi V, Lanteri AP, Minuto A, Santoro V, Ferrea G, Fossa P, Bisio A (2022) Autoxidation products of the methanolic extract of the leaves of Combretum micranthum exert antiviral activity against Tomato Brown Rugose Fruit Virus (ToBRFV). Molecules 27(3):760. https://doi.org/10.3390/molecules27030760
Iobbi V, Santoro V, Maggi N, Giacomini M, Lanteri AP, Minuto G, Minuto A, Fossa P, Tommasi N, Bisio A, Drava G (2023) Characterization of sulfur compounds and antiviral activity against Tomato brown rugose fruit virus (ToBRFV) of Italian “Vessalico” garlic compared to other cultivars and landrace. LWT Food Sci Technol 174:114411. https://doi.org/10.1016/j.lwt.2022.114411
Juarez-Rodriguez L, Perez-Grajales M, Castro-Brindis R, Segura-Miranda A, Magaña-Lira N, Magdaleno-Villar JJ (2021) Evaluation of doses, application periods and residuality of paclobutrazol in tomato. Bioagro 34:63–74. https://doi.org/10.51372/bioagro341.6
Klap C, Luria N, Smith E, Hadad L, Bakelman E, Sela N, Belausov E, Lachman O, Leibman D, Dombrovsky A (2020) Tomato Brown Rugose Fruit Virus contributes to enhanced Pepino Mosaic Virus titers in tomato plants. Viruses 12(8):879. https://doi.org/10.3390/v12080879
Ling KS, Wintermantel WM, Bledsoe M (2008) Genetic composition of Pepino mosaic virus population in North American greenhouse tomatoes. Plant Dis 92(12):1683–1688. https://doi.org/10.1094/PDIS-92-12-1683
Madhusudhan KN, Vinayarani G, Deepak SA, Niranjana SR, Prakash HS, Singh GP, Sinha AK, Prasad BC (2011) Antiviral activity of plant extracts and other inducers against tobamoviruses infection in bell pepper and tomato plants. Int J Plant Pathol 2:35–42. https://doi.org/10.3923/ijpp.2011.35.42
Menzel W, Knierim D, Winter S, Hamacher J, Heupel M (2019) First report of Tomato brown rugose fruit virus infecting tomato in Germany. New Dis Rep 39:1. https://doi.org/10.5197/j.2044-0588.2019.039.001
Ortiz-Martínez LE, Ochoa-Martínez DL (2022) Elicitors and biostimulants in the production of tomato infected with Tomato brown rugose fruit virus. J Plant Dis Prot 130:351–360. https://doi.org/10.1007/s41348-022-00693-6
Ramirez-Estrada K, Vidal-Limon H, Hidalgo D, Moyano E, Golenioswki M, Cusidó RM, Palazon J (2016) Elicitation, an effective strategy for the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecules 21(2):182. https://doi.org/10.3390/molecules21020182
Restrepo RJ (2007) Practical manual: the A, B, C of organic agriculture and rock flour. Managua, Nicaragua
Rivarez MPS, Vučurović A, Mehle N, Ravnikar M, Kutnjak D (2021) Global advances in tomato virome research: current status and the impact of high-throughput sequencing. Front Microbiol 12(1):1–22. https://doi.org/10.3389/fmicb.2021.671925
Roa JM (2015) Supermagro: the organic fertilizer of the future. Rev Innov Agrícola 1(1):24–27
Rodríguez-Mendoza J, García-Ávila CJ, López-Buenfil JA, Araujo-Ruiz K, Quezada-Salinas A, Cambrón-Crisantos JM, Ochoa-Martínez DL (2019) Identification of Tomato brown rugose fruit virus by RT-PCR from a coding region of replicase (RdRP). Rev Mex Fitopatol 37:345–356. https://doi.org/10.18781/R.MEX.FIT.1902-6
Rubio L, Galipienso L, Ferriol I (2020) Detection of plant viruses and disease management: relevance of genetic diversity and evolution. Front Plant Sci 11:1092. https://doi.org/10.3389/fpls.2020.01092
SAS Institute Inc (2002) The SAS system for windows. Version 9.0. SAS Institute Inc., Cary
Shami AMM, Philip K, Muniandy S (2013) Synergy of antibacterial and antioxidant activities from crude extracts and peptides of selected plant mixture. BMC Complement Altern Med 13:360. https://doi.org/10.1186/1472-6882-13-360
Zellner W, Frantz J, Leisner S (2011) Silicon delays Tobacco ringspot virus systemic symptoms in Nicotiana tabacum. J Plant Physiol 168(15):1866–1869. https://doi.org/10.1016/j.jplph.2011.04.002
Zhang S, Griffiths JS, Marchand G, Bernards MA, Wang A (2022) Tomato brown rugose fruit virus: An emerging and rapidly spreading plant RNA virus that threatens tomato production worldwide. Mol Plant Pathol 23:1262–1277. https://doi.org/10.1111/mpp.13229
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The first author would like to thank the Consejo Mexiquense de Ciencia y Tecnología (COMECyT) for their support of this research.
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Ortiz-Martínez, L.E., Ochoa-Martínez, D.L. & Gutiérrez, J. Elicitors in the production of tomato crop infected with Tomato brown rugose fruit virus and Pepino mosaic virus. J Plant Dis Prot 131, 977–986 (2024). https://doi.org/10.1007/s41348-024-00863-8
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DOI: https://doi.org/10.1007/s41348-024-00863-8