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Isolation and identification of grapevine endophytic bacteria with antagonistic potential against Fomitiporia mediterranea, a pathogen involved in grapevine trunk disease

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Abstract

Background

Grapevine trunk diseases (GTDs) are an economically serious threat, affecting grapevine long-term sustainability and productivity. The use of antagonistic endophytic bacteria can be a promissing approach for GTDs’ management.

Results

253 bacterial endophytes were isolated from the stems and roots of grapevines from 68 geographic locations in eight provinces of Iran. Based on biochemical properties, 22 strains were selected and identified using the 16S rRNA gene. The representative strains belonged to 13 genera of Agrobacterium, Arthrobacter, Bacillus, Chryseobacterium, Klebsiella, Kocuria, Pantoea, Pseudomonas, Rahnella, Rothia, Serratia, Staphylococcus and Variovorax. Using the dual culture method, antagonistic activity of 27 strains from 50 selected ones was shown against Fomitiporia mediterranea, a pathogen of GTDs; in which its hyphal growth was inhibited from 10 to 55%. Among the tested bacteria, Serratia plymuthica GI154, Bacillus sp. GI298 and Pseudomonas fluorescens GI310 showed the highest fungal growth inhibition zones, respectively. Co-treatment of GI154, GI298, and GI310 with F. mediterranea, in planta, could reduce the length of its internal canker by 54.41, 60.16 and 49.42% in compare with contol, respectively.

Conclusions

To the best of our knowledge, this is the first report of isolation of Arthrobacter agilis, Chryseobacterium daecheongense, Rahnella aquatilis, Serratia plymuthica, Staphylococcus epidermidis, Staphylococcus warneri and Variovorax paradoxus from grapevine in Iran. Moreover, this is the first report of Rothia amarae as a bacterial endophyte of the grapevine for the world. Grapevine bacterial endophytes exhibit auspicious biocontrol activities that can be utilized in sustainable and organic agriculture.

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Availability of data and materials

Almost all data generated or analyzed related to this topic, are included in this published article. The datasets which was not used directly in this article are available from the corresponding author on reasonable request.

Abbreviations

GTDs:

Grapevine trunk diseases

BCAs:

Biological control agents

NA:

Nutrient agar

HR:

Hypersensitive response

NB:

Nutrient broth

CTAB:

Cetyl trimethyl ammonium bromide

RH:

Relative humidity

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Acknowledgements

We would like to thank Masoud Amir Maafi (AREEO, Iran) and Shahram Shahrokhi (AREEO, Iran) for providing the phytotron facility.

Funding

This research project was sponsored by Iranian Research institute of Plant Protection and Islamic Azad University (North Tehran Branch).

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran

    Seyedeh Elham Vaghari Souran & Fatemeh Ashrafi

  2. Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

    Azam Shekariesfahlan, Shahram Naeimi & Abolghasem Ghasemi

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  1. Seyedeh Elham Vaghari Souran
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  2. Azam Shekariesfahlan
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  3. Fatemeh Ashrafi
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Contributions

Conceptualization, AS, SEVS, SN and AG; methodology, SEVS, AS and AG; software, AS, SEVS, and AG; validation, AS, SEVS, SN, FA and AG; data analysis, SEVS, AS and AG; investigation, SEVS and AS; resources, AS, SEVS, and AG; data curation, AS; writing-original draft preparation, SEVS; writing-review and editing, AS and SN; supervision, AS and FA; funding acquisition, AS and SEVS. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Azam Shekariesfahlan.

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Vaghari Souran, S.E., Shekariesfahlan, A., Ashrafi, F. et al. Isolation and identification of grapevine endophytic bacteria with antagonistic potential against Fomitiporia mediterranea, a pathogen involved in grapevine trunk disease. J Plant Dis Prot 130, 1371–1384 (2023). https://doi.org/10.1007/s41348-023-00788-8

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